dbPAF Protein Information


Tag Content
dbPAF ID dbPAF-0001377
Uniprot Accession O42709; MCM10_SCHPO;
Genbank Protein ID NP_596702.1;
Genbank Nucleotide ID NM_001022626.2;
Protein Name DNA replication licensing factor mcm10
Protein Synonyms/Alias Cdc23 protein;Minichromosome maintenance protein 10;
Gene Name mcm10
Gene Synonyms/Alias cdc23;SPBC1347.10;
Organism Schizosaccharomyces pombe (strain 972 / ATCC 24843)(Fission yeast)
NCBI Taxa ID 284812
Functional Description
(View all)
Required for DNA synthesis. Required for entry into or completion of S phase. Involved in DNA replication and seems to participate in the activation of the pre-replication complex (pre-RC) and in transcription elongation. May play a role as key coordinator in assembling the replication fork. Proposed to function at replication origins following the binding of the mcm2-7 complex prior to the recruitment of sna41. Probably is required to stimulate phosphorylation of the mcm2-7 complex by the dfp1-hsk1 kinase complex. May recruit the DNA polymerase alpha:primase complex to replication origins and is required to maintain it on chromatin independently of sna41. May have primase activity. Binds to single-stranded DNA.
Phosphorylation Sites
dbPAF PTMs: 1
PositionPeptidesSourceReferences ( PMIDs )
64VRVLTVSSPSKLKSPcurated22817900
Sequence
(Fasta)
MHDPFIAEEN DLDLEEKRLQ RQLNEIQEKK RLRSAQKEAS SENAEVIQVP RSPPQQVRVL 60
TVSSPSKLKS PKRLILGIDK GKTGKDVSLG KGPRGPLPKP FHERLAEARN QERKRSDKLK 120
TMKKNRKQSF QRKRNILEDG KSEEEKFPMK CDEIDPYSRQ AIVIRYISDE VAKENIGGNQ 180
VYLIHQLLKL VRAPKFEAPE VDNYVVMGIV ASNSGTRETV NGNKYCMLTL TDLKWQLECF 240
LFGKAFERYW KIQSGTVIAL LNPEVLKPKN PDIGRFSLKL DSEYDVLLEI GRSKHLGYCS 300
SRRKSGELCK HWLDKRAGDV CEYHVDLAVQ RSMSTRTEFA SSMATMHEPR ARREKRFRGQ 360
GFQGYFAGEK YSAIPNAVAG LYDAEDAVQT ERERKERYKK QRAQAERERE ILVRLSKRCC 420
ASSSSSSNSN NLSTGMSMRT LGHQYLNLQG SGVKNLHDKG NPTALSKDSE IDSSTKKPSV 480
LASFNASIMN PKSSLPSFSN SAILGTNDAA SGTPVPQDTT STKVSPAVVF TSSPRIFSPQ 540
SLRKIGFDPT HSADASTTHS TATGLSRSGS LKNIKFRYEF TESDDEDDLE IVP 594
Keyword

KW-0131--Cell cycle
KW-0132--Cell division
KW-0181--Complete proteome
KW-0235--DNA replication
KW-0238--DNA-binding
KW-0479--Metal-binding
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR015408--Znf_Mcm10/DnaG

PROSITE
Pfam

PF09329--zf-primase

Gene Ontology

GO:0005737--C:cytoplasm
GO:0072686--C:mitotic spindle
GO:0000790--C:nuclear chromatin
GO:0043596--C:nuclear replication fork
GO:0031298--C:replication fork protection complex
GO:0035861--C:site of double-strand break
GO:0003896--F:DNA primase activity
GO:0003688--F:DNA replication origin binding
GO:0003690--F:double-stranded DNA binding
GO:0046872--F:metal ion binding
GO:0003697--F:single-stranded DNA binding
GO:0007049--P:cell cycle
GO:0051301--P:cell division
GO:0006270--P:DNA replication initiation
GO:0006269--P:DNA replication, synthesis of RNA primer
GO:1903934--P:positive regulation of DNA primase activity
GO:1900264--P:positive regulation of DNA-directed DNA polymerase activity