dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0001371
Uniprot Accession O42643; PRP22_SCHPO;
Genbank Protein ID NP_593253.1;
Genbank Nucleotide ID NM_001018650.2;
Protein Name Pre-mRNA-splicing factor ATP-dependent RNA helicase prp22
Protein Synonyms/Alias
Gene Name prp22
Gene Synonyms/Alias SPAC10F6.02c;
Organism Schizosaccharomyces pombe (strain 972 / ATCC 24843)(Fission yeast)
NCBI Taxa ID 284812
Functional Description
(View all)		 Acts late in the splicing of pre-mRNA. Required for the splicing of introns with a branch nucleotide to 3'-splice site distance greater or equal to 15. Mediates the release of the spliced mRNA from spliceosomes.
Phosphorylation Sites
 dbPAF PTMs: 2
PositionPeptidesSourceReferences ( PMIDs )
175ERRERSISPSFSHHScurated22817900
402TKVSLKLSPIKVVKAdbPTM 3.0;SysPTM 2.0;curated18257517; 16381945; 23193290; 19366988; 22817900
Sequence
(Fasta)		MDDLKELEYL SLVSKVASEI RNHTGIDDNT LAEFIINLHD QSKNYDEFKN NVLSCGGEFT 60
DSFLQNISRL IKEIKPKDDI PTDNVNNGSN SVNGASHDLD SKDVDKQHQR KMFPGLSIPN 120
STNNLRDRPA LMDNAMDELE ELSTLAKTRR NDRDSRRDER HYLNGIRERR ERSISPSFSH 180
HSRTSISGQS HSSRSSRGPL LNAPTLYGIY SGVVSGIKDF GAFVTLDGFR KRTDGLVHIS 240
NIQLNGRLDH PSEAVSYGQP VFVKVIRIDE SAKRISLSMK EVNQVTGEDL NPDQVSRSTK 300
KGSGANAIPL SAQNSEIGHV NPLETFTSNG RKRLTSPEIW ELQQLAASGA ISATDIPELN 360
DGFNTNNAAE INPEDDEDVE IELREEEPGF LAGQTKVSLK LSPIKVVKAP DGSLSRAAMQ 420
GQILANDRRE IRQKEAKLKS EQEMEKQDLS LSWQDTMSNP QDRKFAQDVR DSAARQLTSE 480
TPSWRQATRN ANISYGKRTT LSMKEQREGL PVFKLRKQFL EAVSKNQILV LLGETGSGKT 540
TQITQYLAEE GYTSDSKMIG CTQPRRVAAM SVAKRVAEEV GCRVGEEVGY TIRFEDKTSR 600
MTQIKYMTDG MLQRECLVDP LLSKYSVIIL DEAHERTVAT DVLFGLLKGT VLKRPDLKLI 660
VTSATLDAER FSSYFYKCPI FTIPGRSYPV EIMYTKQPEA DYLDAALMTV MQIHLSEGPG 720
DILVFLTGQE EIDTSCEILY ERSKMLGDSI PELVILPVYS ALPSEIQSRI FEPAPPGGRK 780
VVIATNIAET SLTIDGIYYV VDPGFVKQSC FDPKLGMDSL IVTPISQAQA RQRSGRAGRT 840
GPGKCYRLYT ESAYRNEMLP SPIPEIQRQN LSHTILMLKA MGINDLLNFD FMDPPPAQTM 900
IAALQNLYAL SALDDEGLLT PLGRKMADFP MEPQLSKVLI TSVELGCSEE MLSIIAMLSV 960
PNIWSRPREK QQEADRQRAQ FANPESDHLT LLNVYTTWKM NRCSDNWCYE HYIQARGMRR 1020
AEDVRKQLIR LMDRYRHPVV SCGRKRELIL RALCSGYFTN VAKRDSHEGC YKTIVENAPV 1080
YMHPSGVLFG KAAEWVIYHE LIQTSKEYMH TVSTVNPKWL VEVAPTFFKF ANANQVSKTK 1140
KNLKVLPLYN RFEKPDEWRI SKQRKGGR 1169
Keyword

KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0347--Helicase
KW-0378--Hydrolase
KW-0507--mRNA processing
KW-0508--mRNA splicing
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-1185--Reference proteome
Interpro

IPR011545--DEAD/DEAH_box_helicase_dom
IPR002464--DNA/RNA_helicase_DEAH_CS
IPR011709--DUF1605
IPR007502--Helicase-assoc_dom
IPR014001--Helicase_ATP-bd
IPR001650--Helicase_C
IPR012340--NA-bd_OB-fold
IPR027417--P-loop_NTPase
IPR022967--S1_dom
IPR003029--S1_domain
PROSITE

PS00690--DEAH_ATP_HELICASE
PS51192--HELICASE_ATP_BIND_1
PS51194--HELICASE_CTER
PS50126--S1
Pfam

PF00270--DEAD
PF04408--HA2
PF00271--Helicase_C
PF07717--OB_NTP_bind
PF00575--S1
Gene Ontology

GO:0005737--C:cytoplasm
GO:0005634--C:nucleus
GO:0005681--C:spliceosomal complex
GO:0005684--C:U2-type spliceosomal complex
GO:0005524--F:ATP binding
GO:0004004--F:ATP-dependent RNA helicase activity
GO:0044822--F:poly(A) RNA binding
GO:0006396--P:RNA processing
GO:0000390--P:spliceosomal complex disassembly