dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0001338
Uniprot Accession O35889; AFAD_RAT; O35890;
Genbank Protein ID NP_037349.1;
Genbank Nucleotide ID NM_013217.2;
Protein Name Afadin
Protein Synonyms/Alias Protein Af-6;
Gene Name Mllt4
Gene Synonyms/Alias Af6;
Organism Rattus norvegicus(Rat)
NCBI Taxa ID 10116
Functional Description
(View all)		 Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton. May play a key role in the organization of epithelial structures of the embryonic ectoderm.
Phosphorylation Sites
 dbPAF PTMs: 35 (View all)
PositionPeptidesSourceReferences ( PMIDs )
203SRLAAEVYKDMPETScurated22276854
216TSFTRTISNPEVVMKPhosphoSitePlus;curated22135298; 22276854
246SSDGRPDSGGTLRIYcurated23984901
391LPYLVELSPGRRNHFPhosphoSitePlus;UniProt22135298; 22673903
424KLYRLQLSVTEVGTEcurated23984901
512DHVLSKRSVDGGLMVPhosphoSitePlus;UniProt22135298; 22673903
557RSTTRLDSDRVSSASdbPTM 3.0;PhosphoSitePlus;curated16641100; 16381945; 23193290; 22135298; 22276854; 23984901
562LDSDRVSSASSTAERdbPTM 3.0;curated18779572; 16381945; 23193290
589QDYRRRESRTQDAAGPhosphoSitePlus22135298
1090ELMTRTSSVVTLEVAdbPTM 3.0;Phospho.ELM 9.0;PhosphoSitePlus;UniProt;curated16452087; 16381945; 23193290; 22135298; 22673903; 23712012;
1114ATLLNQPSPMMQRISPhosphoSitePlus;curated22135298; 22276854
1147NNSAQNGSPESPQMPPhosphoSitePlus;UniProt22135298; 22673903
1150AQNGSPESPQMPWTEPhosphoSitePlus;UniProt;curated22135298; 22673903; 21630457
1179KNRADHRSSPNVANQdbPTM 3.0;PhosphoSitePlus;curated16641100; 16381945; 23193290; 22135298; 21373199; 21630457
1180NRADHRSSPNVANQPdbPTM 3.0;PhosphoSitePlus;curated16641100; 16381945; 23193290; 22135298; 23712012; 21630457
1189NVANQPPSPGGKSPYdbPTM 3.0;PhosphoSitePlus;UniProt;curated16641100; 16381945; 23193290; 17683130; 18779572; 22135298;
1200KSPYTSGTAAKITSVcurated23712012; 21630457
1208AAKITSVSTGNLCTEcurated23984901
1218NLCTEEQTPPPRPEAPhosphoSitePlus;curated22135298; 23712012; 21630457
1237TQTYTREYFTFPASKcurated22276854
Sequence
(Fasta)		MSAGGRDEER RKLADIIHHW NANRLDLFEI SQPTEDLEFH GVMRFYFQDK AAGNFATKCI 60
RVSSTATTQD VIETLAEKFR PDMRMLSSPK YSLYEVHVSG ERRLDIDEKP LVVQLNWNKD 120
DREGRFVLKN ENDAIPAKKA QSNGPEKQEK EGVIQNFKRT LSKKEKKEKK KREKEALRQA 180
SDKEERPSQG DDSENSRLAA EVYKDMPETS FTRTISNPEV VMKRRRQQKL EKRMQEFRSS 240
DGRPDSGGTL RIYADSLKPN IPYKTILLST TDPADFAVAE SLEKYGLEKE NPKDYCIARV 300
MLPPGAQHSD ERGAKEIILD DDECPLQIFR EWPSDKGILV FQLKRRPPDY IPKKMKKHVE 360
GKPLKGKDRA DGSGYGSALP PEKLPYLVEL SPGRRNHFAY YSYHTYEDGS DSRDKPKLYR 420
LQLSVTEVGT EKFDDNSIQL FGPGIQPHHC DLTNMDGVVT VTPRSMDAET YVDGQRISET 480
TMLQSGMRLQ FGTSHVFKFV DPIQDHVLSK RSVDGGLMVK GPRHKPGAVQ ETTFELGGDI 540
HSGTALPASR STTRLDSDRV SSASSTAERG MVKPMIRLDQ EQDYRRRESR TQDAAGPELM 600
LPASIEFRES SEDSFLSAII NYTNSSTVHF KLSPTYVLYM ACRYVLSSQH RPDISPTERT 660
HKAIAVVNKM VSMMEGVIQE VDQVDQKQKN IAGALAFWMA NASELLNFIK QDRDLSRITL 720
DAQDVLAHLV QMAFKYLVHC LQSELNNYMP AFLDDPEENS LQRPKIDDVL HTLTGAMSLL 780
RRCRVNAALT IQLFSQLFHF INMWLFNRLV TDPDSGLCSH YWGAIIRQQL GHIEAWAEKQ 840
GLELAADCHL SRIVQATTLL TMDKYVPDDI PNINSTCFKL NSLQLQALLQ NYHCAPDEPF 900
IPTDLIENVV AVAENTADEL ARSDGRDVQL EEDPDLQLPF LLPEDGYSCD VVRNIPNGLQ 960
EFLDPLCQRG FCRLVPHTRS PGTWTIYFEG ADYESHLMRE NTELTQPLRK EPEVITVTLK 1020
KQNGMGLSIV AAKGAGQDKL GIYVKSVVKG GAADVDGRLA AGDQLLSVDG RSLVGLSQER 1080
AAELMTRTSS VVTLEVAKQG AIYHGLATLL NQPSPMMQRI SDRRGSGKPR PKSEGFELYN 1140
NSAQNGSPES PQMPWTEYSE PKKLPGDDRL MKNRADHRSS PNVANQPPSP GGKSPYTSGT 1200
AAKITSVSTG NLCTEEQTPP PRPEAYPIPT QTYTREYFTF PASKSQDRMA PVQNQWPNYE 1260
EKPHMHTESD HASIAIQRVT RSQEELREEK VYQLERHRVE SGMDRKCDSD MWINQSSSVE 1320
SSTSSQEHLN HSSKSVTPAS TLTKSGPGRW KTPAAVLPTP VAVSQPIRTD LPPPPPPPPA 1380
HYTSDFDGIS MDLPLPPPPA NQAAPQSAQV AAAERKKREE HQRWYEKEKA RLEEERERKR 1440
REQERKLGQM RTQSLNPASF SPLATQAKPE KPSTLQRPQE TVIRELQPQQ QPRTIERRDL 1500
QYITISKEEL SSGDSLSPDP WKRDAREKLE KQQQMHIVDM LSKEIHELQN KGDRTAEESD 1560
RLRKLMLEWQ FQKRLQESKQ KDEDDDEEED DDVDTMLIMQ RLEAERRARL QDEERRRQQQ 1620
LEEMRKREVE DRVRQEEDGR HQEEERVKRD AEEKRRQEEG YYSRLEAERR RQHEEAARRL 1680
LEPEEPGLSR PPLPQDYEPP SQSSAPSAPP PPPQRNASYL KTQVLSPDSL FTAKFVAYDD 1740
DDEEENYVPA GPNSYSGSAG TTAGTYDAPR DTREKLSRSQ DADLPGSSGA PENLTFRERQ 1800
RLFSQGQDVS DKVKASRKLT ELENELNTK
Keyword

KW-0007--Acetylation
KW-0025--Alternative splicing
KW-0130--Cell adhesion
KW-0965--Cell junction
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0903--Direct protein sequencing
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
Interpro

IPR002710--Dilute_dom
IPR000253--FHA_dom
IPR001478--PDZ
IPR000159--RA_dom
IPR008984--SMAD_FHA_domain
IPR029071--Ubiquitin-rel_dom
PROSITE

PS51126--DILUTE
PS50106--PDZ
PS50200--RA
Pfam

PF01843--DIL
PF00498--FHA
PF00595--PDZ
PF00788--RA
Gene Ontology

GO:0005913--C:cell-cell adherens junction
GO:0005829--C:cytosol
GO:0030274--F:LIM domain binding
GO:0007155--P:cell adhesion
GO:0007165--P:signal transduction