dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0001315
Uniprot Accession O35789; B3GA1_RAT;
Genbank Protein ID NP_446455.1; XP_006242795.1; XP_006242796.1;
Genbank Nucleotide ID NM_054003.1; XM_006242733.2; XM_006242734.2;
Protein Name Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
Protein Synonyms/Alias Beta-1,3-glucuronyltransferase 1;Glucuronosyltransferase P;GlcAT-P;UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase;GlcUAT-P;
Gene Name B3gat1
Gene Synonyms/Alias
Organism Rattus norvegicus(Rat)
NCBI Taxa ID 10116
Functional Description
(View all)		 Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.
Phosphorylation Sites
 dbPAF PTMs: 2
PositionPeptidesSourceReferences ( PMIDs )
103PVQKAELTRMANTLLdbPTM 3.0;PhosphoSitePlus;SysPTM 2.0;UniProt16641100; 16381945; 23193290; 22135298; 19366988
108ELTRMANTLLHVPNLdbPTM 3.0;PhosphoSitePlus;SysPTM 2.0;UniProt16641100; 16381945; 23193290; 22135298; 19366988
Sequence
(Fasta)		MPKRRDILAI VLIVLPWTLL ITVWHQSSLA PLLAVHKDEG SDPRHEAPPG ADPREYCMSD 60
RDIVEVVRTE YVYTRPPPWS DTLPTIHVVT PTYSRPVQKA ELTRMANTLL HVPNLHWLVV 120
EDAPRRTPLT ARLLRDTGLN YTHLHVETPR NYKLRGDARD PRIPRGTMQR NLALRWLRET 180
FPRNSTQPGV VYFADDDNTY SLELFEEMRS TRRVSVWPVA FVGGLRYEAP RVNGAGKVVG 240
WKTVFDPHRP FAIDMAGFAV NLRLILQRSQ AYFKLRGVKG GYQESSLLRE LVTLNDLEPK 300
AANCTKILVW HTRTEKPVLV NEGKKGFTDP SVEI 335
Keyword

KW-0181--Complete proteome
KW-0903--Direct protein sequencing
KW-0325--Glycoprotein
KW-0333--Golgi apparatus
KW-0464--Manganese
KW-0472--Membrane
KW-0479--Metal-binding
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0735--Signal-anchor
KW-0808--Transferase
KW-0812--Transmembrane
KW-1133--Transmembrane helix
Interpro

IPR005027--Glyco_trans_43
IPR029044--Nucleotide-diphossugar_trans
PROSITE
Pfam

PF03360--Glyco_transf_43
Gene Ontology

GO:0005796--C:Golgi lumen
GO:0000139--C:Golgi membrane
GO:0016021--C:integral component of membrane
GO:0015018--F:galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity
GO:0046872--F:metal ion binding
GO:0008499--F:UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity
GO:0005975--P:carbohydrate metabolic process
GO:0030204--P:chondroitin sulfate metabolic process
GO:0050650--P:chondroitin sulfate proteoglycan biosynthetic process
GO:0006486--P:protein glycosylation