| Tag |
Content |
| dbPAF ID |
dbPAF-0001311 |
| Uniprot Accession |
O35780; BHE40_RAT;
|
| Genbank Protein ID |
NP_445780.1;
|
| Genbank Nucleotide ID |
NM_053328.1;
|
| Protein Name |
Class E basic helix-loop-helix protein 40 |
| Protein Synonyms/Alias |
|
| Gene Name |
Bhlhe40 |
| Gene Synonyms/Alias |
Bhlhb2;Sharp2; |
| Organism |
Rattus norvegicus(Rat) |
| NCBI Taxa ID |
10116 |
Functional Description
(View all) |
Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA and NR1H3/LXRA transactivation activity. May function as a transcriptional factor for neuronal differentiation (By similarity).Functional Description
Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA and NR1H3/LXRA transactivation activity. May function as a transcriptional factor for neuronal differentiation (By similarity). |
Phosphorylation Sites
|
dbPAF PTMs: 1 | Position | Peptides | Source | References ( PMIDs ) |
|---|
| 383 | LLPQRLPSPLAHSSL | PhosphoSitePlus;UniProt;curated | 22135298; 22673903; 23984901 |
|
Sequence
(Fasta) | MERIPSAQPP PTCLPKTPGL EHGDLSGMDF AHMYQVYKSR RGIKRSEDSK ETYKLPHRLI 60
EKKRRDRINE CIAQLKDLLP EHLKLTTLGH LEKAVVLELT LKHVKALTNL IDQQQQKIMA 120
LQSGLQAGDL SGKNIEAGQE MFCSGFQTCA REVLQYLAKH ENTRDLKSSQ LVTHLHRVVS 180
ELLQGSASRK PLDSAPKPVD FKEKPSFLAK GSEGPGKNCV PVIQRTFAPS GGEQSGSDTD 240
TDSGYGGELE KGDLRSEQPY FKSDHGRRFT VGERVSTIKQ ESEEPPTKKS RMQLSDEEGH 300
FVGSDLMGSP FLGPHPHQPP FCLPFYLIPP SATAYLPMLE KCWYPTSVPL LYPGLNTSAA 360
ALSSFMNPDK IPTPLLLPQR LPSPLAHSSL DSSALLQALK QIPPLNLETK D
412Fasta Sequence
>O35780|Bhlhe40|Rattus norvegicus(Rat)
MERIPSAQPPPTCLPKTPGLEHGDLSGMDFAHMYQVYKSRRGIKRSEDSKETYKLPHRLIEKKRRDRINECIAQLKDLLPEHLKLTTLGHLEKAVVLELTLKHVKALTNLIDQQQQKIMALQSGLQAGDLSGKNIEAGQEMFCSGFQTCAREVLQYLAKHENTRDLKSSQLVTHLHRVVSELLQGSASRKPLDSAPKPVDFKEKPSFLAKGSEGPGKNCVPVIQRTFAPSGGEQSGSDTDTDSGYGGELEKGDLRSEQPYFKSDHGRRFTVGERVSTIKQESEEPPTKKSRMQLSDEEGHFVGSDLMGSPFLGPHPHQPPFCLPFYLIPPSATAYLPMLEKCWYPTSVPLLYPGLNTSAAALSSFMNPDKIPTPLLLPQRLPSPLAHSSLDSSALLQALKQIPPLNLETKD
|
| Keyword |
KW-0090--Biological rhythms
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0238--DNA-binding
KW-1017--Isopeptide bond
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0678--Repressor
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0832--Ubl conjugation
|
| Interpro |
IPR011598--bHLH_dom
IPR003650--Orange_dom
|
| PROSITE |
PS50888--BHLH
PS51054--ORANGE
|
| Pfam |
PF07527--Hairy_orange
PF00010--HLH
|
| Gene Ontology |
GO:0005737--C:cytoplasm
GO:0005730--C:nucleolus
GO:0005634--C:nucleus
GO:0070888--F:E-box binding
GO:0003714--F:transcription corepressor activity
GO:0007623--P:circadian rhythm
GO:0009649--P:entrainment of circadian clock
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter
GO:0045892--P:negative regulation of transcription, DNA-templated
GO:0007399--P:nervous system development
GO:0048168--P:regulation of neuronal synaptic plasticity
GO:0009416--P:response to light stimulus
GO:0006351--P:transcription, DNA-templated
|
