dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0001179
Uniprot Accession O35274; NEB2_RAT;
Genbank Protein ID NP_445926.1;
Genbank Nucleotide ID NM_053474.1;
Protein Name Neurabin-2
Protein Synonyms/Alias Neurabin-II;Neural tissue-specific F-actin-binding protein II;PP1bp134;Protein phosphatase 1 regulatory subunit 9B;Spinophilin;p130;
Gene Name Ppp1r9b
Gene Synonyms/Alias
Organism Rattus norvegicus(Rat)
NCBI Taxa ID 10116
Functional Description
(View all)		 Seems to act as a scaffold protein in multiple signaling pathways. Modulates excitatory synaptic transmission and dendritic spine morphology. Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along the sides of the F-actin. May play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. Believed to target protein phosphatase 1/PP1 to dendritic spines, which are rich in F-actin, and regulates its specificity toward ion channels and other substrates, such as AMPA-type and NMDA-type glutamate receptors. Plays a role in regulation of G-protein coupled receptor signaling, including dopamine D2 receptors and alpha-adrenergic receptors. May establish a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Binds to ADRA1B and RGS2 and mediates regulation of ADRA1B signaling. May confer to Rac signaling specificity by binding to both, RacGEFs and Rac effector proteins. Probably regulates p70 S6 kinase activity by forming a complex with TIAM1. Required for hepatocyte growth factor (HGF)-induced cell migration (By similarity).
Phosphorylation Sites
 dbPAF PTMs: 9
PositionPeptidesSourceReferences ( PMIDs )
15GPGGPLRSASPHRSAcurated23984901
94SDRGVRLSLPRASSLdbPTM 3.0;PhosphoSitePlus12417592; 16381945; 23193290; 18367453; 22135298
99RLSLPRASSLNENVDdbPTM 3.0;PhosphoSitePlus19896464; 16381945; 23193290; 22135298
100LSLPRASSLNENVDHdbPTM 3.0;Phospho.ELM 9.0;PhosphoSitePlus;curated12417592; 15228588; 16381945; 23193290; 19896464; 22135298;
116ALLKLGTSVSERVSRdbPTM 3.0;Phospho.ELM 9.015228588; 16381945; 23193290
177LLRQERASLQDRKLDdbPTM 3.0;PhosphoSitePlus12417592; 16381945; 23193290; 18367453; 22135298
192VVVRFNGSTEALDKLPhosphoSitePlus;curated22135298; 23712012; 21630457; 23984901
193VVRFNGSTEALDKLDcurated25532521; 23712012; 21630457
205KLDADAVSPTVSQLSPhosphoSitePlus;UniProt;curated22135298; 22673903; 23984901
Sequence
(Fasta)		MMKTEPRGPG GPLRSASPHR SAYEAGIQAL KPPDAPGPDE APKAAHHKKY GSNVHRIKSM 60
FLQMGTTTGP PGEAGGASGM AEAPRASDRG VRLSLPRASS LNENVDHSAL LKLGTSVSER 120
VSRFDSKPAP SAQPAPPPHP PSRLQETRKL FERSVPAASG GDKEAVARRL LRQERASLQD 180
RKLDVVVRFN GSTEALDKLD ADAVSPTVSQ LSAVFEKADS RTGLHRAPGP PRAAGAPQVN 240
SKLVTKRSRV FQPPPPPPAP SGDAATEKDR GPGGQQPPQH RVAPARPPPK PREVRKIKPV 300
EVEESGESEA ESAPGEVIQA EVTVHAALEN GSTTATTASP APEEPKAEAV PEEEASSSVA 360
TLERGVDNGR APDMAPEEVD ESKKEDFSEA DLVDVSAYSG LGEDSGGSAL EEDDEEDEED 420
GEPPYEPESG CVEIPGLSEE EDPAPSRKIH FSTAPIQVFS TYSNEDYDRR NEDVDPMAAS 480
AEYELEKRVE RLELFPVELE KDSEGLGISI IGMGAGADMG LEKLGIFVKT VTEGGAAHRD 540
GRIQVNDLLV EVDGTSLVGV TQSFAASVLR NTKGRVRFMI GRERPGEQSE VAQLIQQTLE 600
QERWQREMME QRYAQYGEDD EETGEYATDE DEELSPTFPG GEMAIEVFEL AENEDALSPV 660
EMEPEKLVHK FKELQIKHAV TEAEIQQLKR KLQSLEQEKG RWRVEKAQLE QSVEENKERM 720
EKLEGYWGEA QSLCQAVDEH LRETQAQYQA LERKYSKAKR LIKDYQQKEI EFLKKETAQR 780
RVLEESELAR KEEMDKLLDK ISELEGNLQT LRNSNST 818
Keyword

KW-0002--3D-structure
KW-0009--Actin-binding
KW-0965--Cell junction
KW-1003--Cell membrane
KW-0966--Cell projection
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0206--Cytoskeleton
KW-0217--Developmental protein
KW-0221--Differentiation
KW-0903--Direct protein sequencing
KW-0472--Membrane
KW-0524--Neurogenesis
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0770--Synapse
Interpro

IPR029921--NEB2
IPR001478--PDZ
PROSITE

PS50106--PDZ
Pfam

PF00595--PDZ
Gene Ontology

GO:0015629--C:actin cytoskeleton
GO:0005912--C:adherens junction
GO:0030864--C:cortical actin cytoskeleton
GO:0005737--C:cytoplasm
GO:1990780--C:cytoplasmic side of dendritic spine plasma membrane
GO:0043197--C:dendritic spine
GO:0044327--C:dendritic spine head
GO:0032591--C:dendritic spine membrane
GO:0044326--C:dendritic spine neck
GO:0030175--C:filopodium
GO:0030426--C:growth cone
GO:0030027--C:lamellipodium
GO:0043005--C:neuron projection
GO:0043025--C:neuronal cell body
GO:0005634--C:nucleus
GO:0005886--C:plasma membrane
GO:0014069--C:postsynaptic density
GO:0032587--C:ruffle membrane
GO:0003779--F:actin binding
GO:0051015--F:actin filament binding
GO:0031749--F:D2 dopamine receptor binding
GO:0044325--F:ion channel binding
GO:0019900--F:kinase binding
GO:0008022--F:protein C-terminus binding
GO:0032403--F:protein complex binding
GO:0004672--F:protein kinase activity
GO:0008157--F:protein phosphatase 1 binding
GO:0004864--F:protein phosphatase inhibitor activity
GO:0030042--P:actin filament depolymerization
GO:0007568--P:aging
GO:0019722--P:calcium-mediated signaling
GO:0016477--P:cell migration
GO:0035690--P:cellular response to drug
GO:0071364--P:cellular response to epidermal growth factor stimulus
GO:0071392--P:cellular response to estradiol stimulus
GO:0071482--P:cellular response to light stimulus
GO:0071315--P:cellular response to morphine
GO:0071407--P:cellular response to organic cyclic compound
GO:1901653--P:cellular response to peptide
GO:0021987--P:cerebral cortex development
GO:0016358--P:dendrite development
GO:0003006--P:developmental process involved in reproduction
GO:0046847--P:filopodium assembly
GO:0021766--P:hippocampus development
GO:0007612--P:learning
GO:0060179--P:male mating behavior
GO:0050804--P:modulation of synaptic transmission
GO:0032515--P:negative regulation of phosphoprotein phosphatase activity
GO:0031175--P:neuron projection development
GO:1903829--P:positive regulation of cellular protein localization
GO:0090004--P:positive regulation of establishment of protein localization to plasma membrane
GO:1904372--P:positive regulation of protein localization to actin cortical patch
GO:1903119--P:protein localization to actin cytoskeleton
GO:1990778--P:protein localization to cell periphery
GO:0006468--P:protein phosphorylation
GO:0042127--P:regulation of cell proliferation
GO:2000474--P:regulation of opioid receptor signaling pathway
GO:0001932--P:regulation of protein phosphorylation
GO:0061458--P:reproductive system development
GO:0043200--P:response to amino acid
GO:0001975--P:response to amphetamine
GO:0036276--P:response to antidepressant
GO:0097338--P:response to clozapine
GO:0042493--P:response to drug
GO:0032355--P:response to estradiol
GO:0035902--P:response to immobilization stress
GO:1904373--P:response to kainic acid
GO:1904386--P:response to L-phenylalanine derivative
GO:0035094--P:response to nicotine
GO:0014070--P:response to organic cyclic compound
GO:0010243--P:response to organonitrogen compound
GO:0034695--P:response to prostaglandin E
GO:0048545--P:response to steroid hormone