dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000931
Uniprot Accession O15344; TRI18_HUMAN; B2RCG2; O75361; Q9BZX5;
Genbank Protein ID NP_000372.1; NP_001092094.1; NP_001180206.1; NP_001180207.1; NP_001180208.1; NP_001180209.1; NP_001180210.1; NP_150632.1;
Genbank Nucleotide ID NM_000381.3; NM_001098624.2; NM_001193277.1; NM_001193278.1; NM_001193279.1; NM_001193280.1; NM_001193281.1; NM_033290.3;
Protein Name E3 ubiquitin-protein ligase Midline-1
Protein Synonyms/Alias Midin;Putative transcription factor XPRF;RING finger protein 59;RING finger protein Midline-1;Tripartite motif-containing protein 18;
Gene Name MID1
Gene Synonyms/Alias FXY;RNF59;TRIM18;XPRF;
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)		 Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.
Phosphorylation Sites
 dbPAF PTMs: 18
PositionPeptidesSourceReferences ( PMIDs )
64PTCRHVITLSQRGLDcurated20873877
66CRHVITLSQRGLDGLcurated20873877; 24961811
78DGLKRNVTLQNIIDRPhosphoSitePlus22135298
90IDRFQKASVSGPNSPdbPTM 3.0;HPRD 9;PhosphoSitePlus;curated19413330; 16381945; 23193290; 20068231; 18988627; 22135298;
92RFQKASVSGPNSPSEdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PHOSIDA;PhosphoSitePlus;SysPTM 2.0;UniProt;curated17081983; 19413330; 16381945; 23193290; 18669648; 20363803;
96ASVSGPNSPSETRREdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoPep 2.0;PhosphoSitePlus;curated19413330; 16381945; 23193290; 18669648; 19664995; 20363803;
98VSGPNSPSETRRERAdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PHOSIDA;PhosphoSitePlus;SysPTM 2.0;curated17081983; 19413330; 16381945; 23193290; 20068231; 19651622;
213DHQVAALSERYDKLKPhosphoSitePlus;curated22135298; 25627689
311QCIERSASLISQAEHPhosphoSitePlus;curated22135298; 25627689; 23898821
378KLLECLDYLTAPNPPPhosphoSitePlus22135298
500AHRKLKVSHDNLTVEPhosphoSitePlus;curated22135298; 20873877
511LTVERDESSSKKSHTPhosphoSitePlus;curated22135298; 25627689; 23898821; 23186163; 20873877; 24961811
512TVERDESSSKKSHTPcurated20873877
513VERDESSSKKSHTPEPhosphoSitePlus;curated22135298; 20873877
518SSSKKSHTPERFTSQPhosphoSitePlus22135298
528RFTSQGSYGVAGNVFPhosphoSitePlus22135298
572HEWIGKNSASWALCRdbPTM 3.0;PhosphoSitePlus;curated20363803; 16381945; 23193290; 22135298; 23663014
590NWVVRHNSKEIPIEPcurated20873877
Sequence
(Fasta)		METLESELTC PICLELFEDP LLLPCAHSLC FNCAHRILVS HCATNESVES ITAFQCPTCR 60
HVITLSQRGL DGLKRNVTLQ NIIDRFQKAS VSGPNSPSET RRERAFDANT MTSAEKVLCQ 120
FCDQDPAQDA VKTCVTCEVS YCDECLKATH PNKKPFTGHR LIEPIPDSHI RGLMCLEHED 180
EKVNMYCVTD DQLICALCKL VGRHRDHQVA ALSERYDKLK QNLESNLTNL IKRNTELETL 240
LAKLIQTCQH VEVNASRQEA KLTEECDLLI EIIQQRRQII GTKIKEGKVM RLRKLAQQIA 300
NCKQCIERSA SLISQAEHSL KENDHARFLQ TAKNITERVS MATASSQVLI PEINLNDTFD 360
TFALDFSREK KLLECLDYLT APNPPTIREE LCTASYDTIT VHWTSDDEFS VVSYELQYTI 420
FTGQANVVSL CNSADSWMIV PNIKQNHYTV HGLQSGTKYI FMVKAINQAG SRSSEPGKLK 480
TNSQPFKLDP KSAHRKLKVS HDNLTVERDE SSSKKSHTPE RFTSQGSYGV AGNVFIDSGR 540
HYWEVVISGS TWYAIGLAYK SAPKHEWIGK NSASWALCRC NNNWVVRHNS KEIPIEPAPH 600
LRRVGILLDY DNGSIAFYDA LNSIHLYTFD VAFAQPVCPT FTVWNKCLTI ITGLPIPDHL 660
DCTEQLP 668
Keyword

KW-0002--3D-structure
KW-0025--Alternative splicing
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0206--Cytoskeleton
KW-0225--Disease mutation
KW-0436--Ligase
KW-0479--Metal-binding
KW-0493--Microtubule
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger
Interpro

IPR001870--B30
2/SPRY--
IPR003649--Bbox_C
IPR003879--Butyrophylin
IPR013320--ConA-like_dom
IPR017903--COS_domain
IPR003961--FN3_dom
IPR013783--Ig-like_fold
IPR027727--MID1
IPR006574--PRY
IPR003877--SPRY_dom
IPR000315--Znf_B-box
IPR001841--Znf_RING
IPR013083--Znf_RING/FYVE/PHD
IPR017907--Znf_RING_CS
PROSITE

PS50188--B302_SPRY
PS51262--COS
PS50853--FN3
PS50119--ZF_BBOX
PS00518--ZF_RING_1
PS50089--ZF_RING_2
Pfam

PF00041--fn3
PF13765--PRY
PF00622--SPRY
PF00643--zf-B_box
Gene Ontology

GO:0005829--C:cytosol
GO:0005874--C:microtubule
GO:0005875--C:microtubule associated complex
GO:0005819--C:spindle
GO:0042802--F:identical protein binding
GO:0016874--F:ligase activity
GO:0008017--F:microtubule binding
GO:0051219--F:phosphoprotein binding
GO:0046982--F:protein heterodimerization activity
GO:0042803--F:protein homodimerization activity
GO:0031625--F:ubiquitin protein ligase binding
GO:0008270--F:zinc ion binding
GO:0019221--P:cytokine-mediated signaling pathway
GO:0060333--P:interferon-gamma-mediated signaling pathway
GO:0000226--P:microtubule cytoskeleton organization
GO:0007026--P:negative regulation of microtubule depolymerization
GO:0007389--P:pattern specification process
GO:0032874--P:positive regulation of stress-activated MAPK cascade
GO:0035372--P:protein localization to microtubule