dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000880
Uniprot Accession O15169; AXIN1_HUMAN; Q4TT26; Q4TT27; Q86YA7; Q8WVW6; Q96S28;
Genbank Protein ID NP_003493.1; NP_851393.1;
Genbank Nucleotide ID NM_003502.3; NM_181050.2;
Protein Name Axin-1
Protein Synonyms/Alias Axis inhibition protein 1;hAxin;
Gene Name AXIN1
Gene Synonyms/Alias AXIN;
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)		 Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development.
Phosphorylation Sites
 dbPAF PTMs: 31 (View all)
PositionPeptidesSourceReferences ( PMIDs )
60KGETSTATPRRSDLDPhosphoSitePlus22135298
75LGYEPEGSASPTPPYdbPTM 3.0;PhosphoSitePlus;curated17318175; 16381945; 23193290; 21406692; 22135298; 25627689;
77YEPEGSASPTPPYLKdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoSitePlus;curated17318175; 16381945; 23193290; 18669648; 21406692; 18988627;
79PEGSASPTPPYLKWAdbPTM 3.0;PhosphoSitePlus;curated21406692; 16381945; 23193290; 22135298; 25627689; 23917254;
82SASPTPPYLKWAESLPhosphoSitePlus22135298
148ARAIYRKYILDNNGIdbPTM 3.0;HPRD 9;PhosphoSitePlus;curated18491316; 16381945; 23193290; 18988627; 22135298; 22817900
160NGIVSRQTKPATKSFdbPTM 3.0;HPRD 9;PhosphoSitePlus;curated18491316; 16381945; 23193290; 18988627; 22135298; 22817900
213IYLEYTRTGSESPKVdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;curated18669648; 16381945; 23193290; 18988627; 22817900
215LEYTRTGSESPKVCScurated25849741
217YTRTGSESPKVCSDQdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoSitePlus;curated17318175; 16381945; 23193290; 18669648; 18988627; 22135298;
281ETAAPRVSSSRRYSEcurated20068231
469AHEENPESILDEHVQdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;curated17318175; 16381945; 23193290; 19060867; 22135298; 23186163;
481HVQRVLRTPGRQSPGdbPTM 3.0;PhosphoSitePlus;curated20363803; 21940452; 16381945; 23193290; 22135298; 23898821;
486LRTPGRQSPGPGHRSPhosphoSitePlus;curated22135298; 23898821; 23186163; 20873877
493SPGPGHRSPDSGHVAdbPTM 3.0;HPRD 9;PhosphoSitePlus;curated20363803; 16381945; 23193290; 19651622; 18988627; 22135298;
511VALGGAASGHGKHVPPhosphoSitePlus;curated22135298; 25627689; 23186163
542VHHHVHHSTARPKEQcurated22798277
560EATRRAQSSFAWGLEcurated20873877
561ATRRAQSSFAWGLEPcurated20873877
570AWGLEPHSHGARSRGcurated20873877
Sequence
(Fasta)		MNIQEQGFPL DLGASFTEDA PRPPVPGEEG ELVSTDPRPA SYSFCSGKGV GIKGETSTAT 60
PRRSDLDLGY EPEGSASPTP PYLKWAESLH SLLDDQDGIS LFRTFLKQEG CADLLDFWFA 120
CTGFRKLEPC DSNEEKRLKL ARAIYRKYIL DNNGIVSRQT KPATKSFIKG CIMKQLIDPA 180
MFDQAQTEIQ ATMEENTYPS FLKSDIYLEY TRTGSESPKV CSDQSSGSGT GKGISGYLPT 240
LNEDEEWKCD QDMDEDDGRD AAPPGRLPQK LLLETAAPRV SSSRRYSEGR EFRYGSWREP 300
VNPYYVNAGY ALAPATSAND SEQQSLSSDA DTLSLTDSSV DGIPPYRIRK QHRREMQESV 360
QVNGRVPLPH IPRTYRVPKE VRVEPQKFAE ELIHRLEAVQ RTREAEEKLE ERLKRVRMEE 420
EGEDGDPSSG PPGPCHKLPP APAWHHFPPR CVDMGCAGLR DAHEENPESI LDEHVQRVLR 480
TPGRQSPGPG HRSPDSGHVA KMPVALGGAA SGHGKHVPKS GAKLDAAGLH HHRHVHHHVH 540
HSTARPKEQV EAEATRRAQS SFAWGLEPHS HGARSRGYSE SVGAAPNASD GLAHSGKVGV 600
ACKRNAKKAE SGKSASTEVP GASEDAEKNQ KIMQWIIEGE KEISRHRRTG HGSSGTRKPQ 660
PHENSRPLSL EHPWAGPQLR TSVQPSHLFI QDPTMPPHPA PNPLTQLEEA RRRLEEEEKR 720
ASRAPSKQRY VQEVMRRGRA CVRPACAPVL HVVPAVSDME LSETETRSQR KVGGGSAQPC 780
DSIVVAYYFC GEPIPYRTLV RGRAVTLGQF KELLTKKGSY RYYFKKVSDE FDCGVVFEEV 840
REDEAVLPVF EEKIIGKVEK VD 863
Keyword

KW-0002--3D-structure
KW-0013--ADP-ribosylation
KW-0025--Alternative splicing
KW-0053--Apoptosis
KW-1003--Cell membrane
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0217--Developmental protein
KW-0225--Disease mutation
KW-1017--Isopeptide bond
KW-0472--Membrane
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0043--Tumor suppressor
KW-0832--Ubl conjugation
KW-0879--Wnt signaling pathway
Interpro

IPR029797--AXIN1
IPR014936--Axin_b-cat-bd
IPR032101--Axin_TNKS_BD
IPR001158--DIX
IPR016137--RGS
IPR024066--RGS_subdom1
IPR029071--Ubiquitin-rel_dom
PROSITE

PS50841--DIX
PS50132--RGS
Pfam

PF16646--AXIN1_TNKS_BD
PF08833--Axin_b-cat_bind
PF00778--DIX
PF00615--RGS
Gene Ontology

GO:0030877--C:beta-catenin destruction complex
GO:0005938--C:cell cortex
GO:0071944--C:cell periphery
GO:0005737--C:cytoplasm
GO:0016023--C:cytoplasmic membrane-bounded vesicle
GO:0005881--C:cytoplasmic microtubule
GO:0031410--C:cytoplasmic vesicle
GO:0005829--C:cytosol
GO:0016328--C:lateral plasma membrane
GO:0005634--C:nucleus
GO:0048471--C:perinuclear region of cytoplasm
GO:0005886--C:plasma membrane
GO:0014069--C:postsynaptic density
GO:0070016--F:armadillo repeat domain binding
GO:0008013--F:beta-catenin binding
GO:0019899--F:enzyme binding
GO:0005096--F:GTPase activator activity
GO:0070411--F:I-SMAD binding
GO:0042802--F:identical protein binding
GO:0032947--F:protein complex scaffold
GO:0042803--F:protein homodimerization activity
GO:0019901--F:protein kinase binding
GO:0004871--F:signal transducer activity
GO:0046332--F:SMAD binding
GO:0031625--F:ubiquitin protein ligase binding
GO:0007257--P:activation of JUN kinase activity
GO:0032147--P:activation of protein kinase activity
GO:0006915--P:apoptotic process
GO:0048320--P:axial mesoderm formation
GO:0060070--P:canonical Wnt signaling pathway
GO:0060823--P:canonical Wnt signaling pathway involved in neural plate anterior/posterior pattern formation
GO:0008219--P:cell death
GO:0030154--P:cell differentiation
GO:0043623--P:cellular protein complex assembly
GO:0071407--P:cellular response to organic cyclic compound
GO:0031122--P:cytoplasmic microtubule organization
GO:0007368--P:determination of left/right symmetry
GO:0009950--P:dorsal/ventral axis specification
GO:0048048--P:embryonic eye morphogenesis
GO:0060272--P:embryonic skeletal joint morphogenesis
GO:0021797--P:forebrain anterior/posterior pattern specification
GO:0071514--P:genetic imprinting
GO:0001701--P:in utero embryonic development
GO:0055001--P:muscle cell development
GO:0090090--P:negative regulation of canonical Wnt signaling pathway
GO:0045599--P:negative regulation of fat cell differentiation
GO:0051248--P:negative regulation of protein metabolic process
GO:0034244--P:negative regulation of transcription elongation from RNA polymerase II promoter
GO:0030178--P:negative regulation of Wnt signaling pathway
GO:0006913--P:nucleocytoplasmic transport
GO:0030910--P:olfactory placode formation
GO:0001743--P:optic placode formation
GO:0090263--P:positive regulation of canonical Wnt signaling pathway
GO:0043547--P:positive regulation of GTPase activity
GO:0046330--P:positive regulation of JNK cascade
GO:0033138--P:positive regulation of peptidyl-serine phosphorylation
GO:0010800--P:positive regulation of peptidyl-threonine phosphorylation
GO:0032436--P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0045732--P:positive regulation of protein catabolic process
GO:0001934--P:positive regulation of protein phosphorylation
GO:0031398--P:positive regulation of protein ubiquitination
GO:2000060--P:positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0045893--P:positive regulation of transcription, DNA-templated
GO:0030511--P:positive regulation of transforming growth factor beta receptor signaling pathway
GO:0051443--P:positive regulation of ubiquitin-protein transferase activity
GO:0036342--P:post-anal tail morphogenesis
GO:0030163--P:protein catabolic process
GO:0051260--P:protein homooligomerization
GO:0000209--P:protein polyubiquitination
GO:0035412--P:regulation of catenin import into nucleus
GO:0007605--P:sensory perception of sound
GO:0090244--P:Wnt signaling pathway involved in somitogenesis
GO:0021881--P:Wnt-activated signaling pathway involved in forebrain neuron fate commitment