dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000878
Uniprot Accession O15164; TIF1A_HUMAN; A4D1R7; A4D1R8; O95854;
Genbank Protein ID NP_003843.3; NP_056989.2;
Genbank Nucleotide ID NM_003852.3; NM_015905.2;
Protein Name Transcription intermediary factor 1-alpha
Protein Synonyms/Alias
Gene Name TRIM24
Gene Synonyms/Alias RNF82;TIF1;TIF1A;
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)		 Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes (By similarity).
Phosphorylation Sites
 dbPAF PTMs: 53 (View all)
PositionPeptidesSourceReferences ( PMIDs )
41ESRQGPDSERGGEAAcurated22817900
101PMLGSAETPPPVPAPdbPTM 3.0;HPRD 9;PHOSIDA;PhosphoSitePlus;UniProt;curated20068231; 16381945; 23193290; 18988627; 21081558; 22135298;
110PPVPAPGSPVSGSSPdbPTM 3.0;HPRD 9;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;UniProt;curated20068231; 16381945; 23193290; 18988627; 21081558; 19060867;
148DNFFVKDTTEVPSSTPhosphoSitePlus22135298
153KDTTEVPSSTVEKSNPhosphoSitePlus22135298
154DTTEVPSSTVEKSNQPhosphoSitePlus22135298
209VRQKEEVSPEAVGVTPhosphoSitePlus;curated22135298; 25627689; 23898821; 24732914
234KKEQLKLYCETCDKLPhosphoSitePlus22135298
452SQPPSGLSSNQLSKFPhosphoSitePlus22135298
590STSSTPSSPTITSAAPhosphoSitePlus;curated22135298; 23917254
606YDGKAFGSPMIDLSSPHOSIDA;PhosphoSitePlus;curated21081558; 22135298; 25627689
612GSPMIDLSSPVGGSYcurated25627689
643DNIVRKDTNIDHGQPcurated22817900
654HGQPRPPSNRTVQSPdbPTM 3.0;PhosphoSitePlus;curated21406692; 16381945; 23193290; 22135298; 25627689; 23917254;
657PRPPSNRTVQSPNSSPhosphoPep 2.019060867
660PSNRTVQSPNSSVPSdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;curated18669648; 16381945; 23193290; 19651622; 18988627; 19060867;
663RTVQSPNSSVPSPGLPhosphoSitePlus;curated22135298; 23186163
664TVQSPNSSVPSPGLAPhosphoSitePlus;curated22135298; 23186163; 25849741
667SPNSSVPSPGLAGPVdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoSitePlus;SysPTM 2.0;UniProt;curated18669648; 16381945; 23193290; 18988627; 22135298; 19366988;
685SVHPPIRSPSASSVGdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoSitePlus;curated18669648; 16381945; 23193290; 18988627; 22135298; 23898821;
Sequence
(Fasta)		MEVAVEKAVA AAAAASAAAS GGPSAAPSGE NEAESRQGPD SERGGEAARL NLLDTCAVCH 60
QNIQSRAPKL LPCLHSFCQR CLPAPQRYLM LPAPMLGSAE TPPPVPAPGS PVSGSSPFAT 120
QVGVIRCPVC SQECAERHII DNFFVKDTTE VPSSTVEKSN QVCTSCEDNA EANGFCVECV 180
EWLCKTCIRA HQRVKFTKDH TVRQKEEVSP EAVGVTSQRP VFCPFHKKEQ LKLYCETCDK 240
LTCRDCQLLE HKEHRYQFIE EAFQNQKVII DTLITKLMEK TKYIKFTGNQ IQNRIIEVNQ 300
NQKQVEQDIK VAIFTLMVEI NKKGKALLHQ LESLAKDHRM KLMQQQQEVA GLSKQLEHVM 360
HFSKWAVSSG SSTALLYSKR LITYRLRHLL RARCDASPVT NNTIQFHCDP SFWAQNIINL 420
GSLVIEDKES QPQMPKQNPV VEQNSQPPSG LSSNQLSKFP TQISLAQLRL QHMQQQVMAQ 480
RQQVQRRPAP VGLPNPRMQG PIQQPSISHQ QPPPRLINFQ NHSPKPNGPV LPPHPQQLRY 540
PPNQNIPRQA IKPNPLQMAF LAQQAIKQWQ ISSGQGTPST TNSTSSTPSS PTITSAAGYD 600
GKAFGSPMID LSSPVGGSYN LPSLPDIDCS STIMLDNIVR KDTNIDHGQP RPPSNRTVQS 660
PNSSVPSPGL AGPVTMTSVH PPIRSPSASS VGSRGSSGSS SKPAGADSTH KVPVVMLEPI 720
RIKQENSGPP ENYDFPVVIV KQESDEESRP QNANYPRSIL TSLLLNSSQS STSEETVLRS 780
DAPDSTGDQP GLHQDNSSNG KSEWLDPSQK SPLHVGETRK EDDPNEDWCA VCQNGGELLC 840
CEKCPKVFHL SCHVPTLTNF PSGEWICTFC RDLSKPEVEY DCDAPSHNSE KKKTEGLVKL 900
TPIDKRKCER LLLFLYCHEM SLAFQDPVPL TVPDYYKIIK NPMDLSTIKK RLQEDYSMYS 960
KPEDFVADFR LIFQNCAEFN EPDSEVANAG IKLENYFEEL LKNLYPEKRF PKPEFRNESE 1020
DNKFSDDSDD DFVQPRKKRL KSIEERQLLK 1051
Keyword

KW-0002--3D-structure
KW-0025--Alternative splicing
KW-0103--Bromodomain
KW-0160--Chromosomal rearrangement
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0903--Direct protein sequencing
KW-0238--DNA-binding
KW-1017--Isopeptide bond
KW-0436--Ligase
KW-0479--Metal-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-0621--Polymorphism
KW-1185--Reference proteome
KW-0677--Repeat
KW-0678--Repressor
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0043--Tumor suppressor
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger
Interpro

IPR003649--Bbox_C
IPR001487--Bromodomain
IPR018359--Bromodomain_CS
IPR019786--Zinc_finger_PHD-type_CS
IPR000315--Znf_B-box
IPR011011--Znf_FYVE_PHD
IPR001965--Znf_PHD
IPR019787--Znf_PHD-finger
IPR001841--Znf_RING
IPR013083--Znf_RING/FYVE/PHD
IPR017907--Znf_RING_CS
PROSITE

PS00633--BROMODOMAIN_1
PS50014--BROMODOMAIN_2
PS50119--ZF_BBOX
PS01359--ZF_PHD_1
PS50016--ZF_PHD_2
PS00518--ZF_RING_1
PS50089--ZF_RING_2
Pfam

PF00439--Bromodomain
PF00628--PHD
PF00643--zf-B_box
Gene Ontology

GO:0005829--C:cytosol
GO:0005719--C:nuclear euchromatin
GO:0005634--C:nucleus
GO:0005726--C:perichromatin fibrils
GO:0003682--F:chromatin binding
GO:0034056--F:estrogen response element binding
GO:0016874--F:ligase activity
GO:0070577--F:lysine-acetylated histone binding
GO:0002039--F:p53 binding
GO:0004672--F:protein kinase activity
GO:0005102--F:receptor binding
GO:0043565--F:sequence-specific DNA binding
GO:0003713--F:transcription coactivator activity
GO:0061630--F:ubiquitin protein ligase activity
GO:0004842--F:ubiquitin-protein transferase activity
GO:0008270--F:zinc ion binding
GO:0055074--P:calcium ion homeostasis
GO:0071391--P:cellular response to estrogen stimulus
GO:0008285--P:negative regulation of cell proliferation
GO:0045892--P:negative regulation of transcription, DNA-templated
GO:0045893--P:positive regulation of transcription, DNA-templated
GO:0046777--P:protein autophosphorylation
GO:0030163--P:protein catabolic process
GO:0016567--P:protein ubiquitination
GO:0042981--P:regulation of apoptotic process
GO:0031647--P:regulation of protein stability
GO:1901796--P:regulation of signal transduction by p53 class mediator
GO:0070562--P:regulation of vitamin D receptor signaling pathway
GO:0006366--P:transcription from RNA polymerase II promoter