dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000803
Uniprot Accession O14964; HGS_HUMAN; Q9NR36;
Genbank Protein ID NP_004703.1;
Genbank Nucleotide ID NM_004712.4;
Protein Name Hepatocyte growth factor-regulated tyrosine kinase substrate
Protein Synonyms/Alias Hrs;Protein pp110;
Gene Name HGS
Gene Synonyms/Alias HRS;
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)		 Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.
Phosphorylation Sites
 dbPAF PTMs: 35 (View all)
PositionPeptidesSourceReferences ( PMIDs )
78VVKNCGQTVHDEVANPHOSIDA21081558
125AFRNEPKYKVVQDTYPhosphoSitePlus;curated22135298; 22817900
131KYKVVQDTYQIMKVEdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;curated21049007; 16381945; 23193290; 19060867; 22135298; 22817900
132YKVVQDTYQIMKVEGdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoPep 2.0;PhosphoSitePlus;curated18083107; 16381945; 23193290; 18180459; 19658100; 19901323;
198GKCSSKYSTIPKFGIdbPTM 3.0;HPRD 9;curated20068231; 16381945; 23193290; 18988627
216VRVCEPCYEQLNRKAdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoPep 2.0;PhosphoSitePlus;UniProt;curated15592455; 18083107; 16381945; 23193290; 16497976; 17360941;
228RKAEGKATSTTELPPPhosphoSitePlus22135298
230AEGKATSTTELPPEYcurated25627689
237TTELPPEYLTSPLSQdbPTM 3.0;PhosphoSitePlus;curated18180459; 20736484; 16381945; 23193290; 22135298; 25627689;
239ELPPEYLTSPLSQQSPhosphoSitePlus;curated22135298; 25627689
240LPPEYLTSPLSQQSQdbPTM 3.0;HPRD 9;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;curated19413330; 16381945; 23193290; 16964243; 18988627; 19060867;
243EYLTSPLSQQSQLPPdbPTM 3.0;PhosphoSitePlus;curated16964243; 16381945; 23193290; 22135298; 22817900
246TSPLSQQSQLPPKRDPhosphoSitePlus22135298
284KERLRQKSTYTSYPKPhosphoSitePlus22135298
286RLRQKSTYTSYPKAEdbPTM 3.0;PhosphoSitePlus;curated19534553; 16381945; 23193290; 20007894; 20736484; 22135298;
287LRQKSTYTSYPKAEPPhosphoSitePlus22135298
288RQKSTYTSYPKAEPMPhosphoSitePlus22135298
289QKSTYTSYPKAEPMPdbPTM 3.0;PHOSIDA;PhosphoSitePlus;curated19534553; 16381945; 23193290; 21081558; 22135298; 25627689;
305ASSAPPASSLYSSPVPhosphoSitePlus;curated22135298; 25627689
306SSAPPASSLYSSPVNPhosphoPep 2.0;PhosphoSitePlus;curated19060867; 22135298; 25627689
Sequence
(Fasta)		MGRGSGTFER LLDKATSQLL LETDWESILQ ICDLIRQGDT QAKYAVNSIK KKVNDKNPHV 60
ALYALEVMES VVKNCGQTVH DEVANKQTME ELKDLLKRQV EVNVRNKILY LIQAWAHAFR 120
NEPKYKVVQD TYQIMKVEGH VFPEFKESDA MFAAERAPDW VDAEECHRCR VQFGVMTRKH 180
HCRACGQIFC GKCSSKYSTI PKFGIEKEVR VCEPCYEQLN RKAEGKATST TELPPEYLTS 240
PLSQQSQLPP KRDETALQEE EELQLALALS QSEAEEKERL RQKSTYTSYP KAEPMPSASS 300
APPASSLYSS PVNSSAPLAE DIDPELARYL NRNYWEKKQE EARKSPTPSA PVPLTEPAAQ 360
PGEGHAAPTN VVENPLPETD SQPIPPSGGP FSEPQFHNGE SEESHEQFLK ALQNAVTTFV 420
NRMKSNHMRG RSITNDSAVL SLFQSINGMH PQLLELLNQL DERRLYYEGL QDKLAQIRDA 480
RGALSALREE HREKLRRAAE EAERQRQIQL AQKLEIMRQK KQEYLEVQRQ LAIQRLQEQE 540
KERQMRLEQQ KQTVQMRAQM PAFPLPYAQL QAMPAAGGVL YQPSGPASFP STFSPAGSVE 600
GSPMHGVYMS QPAPAAGPYP SMPSTAADPS MVSAYMYPAG ATGAQAAPQA QAGPTASPAY 660
SSYQPTPTAG YQNVASQAPQ SLPAISQPPQ SSTMGYMGSQ SVSMGYQPYN MQNLMTTLPS 720
QDASLPPQQP YIAGQQPMYQ QMAPSGGPPQ QQPPVAQQPQ AQGPPAQGSE AQLISFD 778
Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0025--Alternative splicing
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0967--Endosome
KW-0472--Membrane
KW-0479--Metal-binding
KW-0597--Phosphoprotein
KW-0621--Polymorphism
KW-0653--Protein transport
KW-1185--Reference proteome
KW-0813--Transport
KW-0832--Ubl conjugation
KW-0862--Zinc
KW-0863--Zinc-finger
Interpro

IPR008942--ENTH_VHS
IPR024641--HRS_helical
IPR017073--Ubi-bd_Hrs_VPS27
IPR003903--UIM_dom
IPR002014--VHS_dom
IPR018205--VHS_subgr
IPR000306--Znf_FYVE
IPR017455--Znf_FYVE-rel
IPR011011--Znf_FYVE_PHD
IPR013083--Znf_RING/FYVE/PHD
PROSITE

PS50330--UIM
PS50179--VHS
PS50178--ZF_FYVE
Pfam

PF01363--FYVE
PF12210--Hrs_helical
PF00790--VHS
Gene Ontology

GO:0005737--C:cytoplasm
GO:0005829--C:cytosol
GO:0005769--C:early endosome
GO:0031901--C:early endosome membrane
GO:0005768--C:endosome
GO:0070062--C:extracellular exosome
GO:0043231--C:intracellular membrane-bounded organelle
GO:0032585--C:multivesicular body membrane
GO:0030141--C:secretory granule
GO:0046872--F:metal ion binding
GO:0019904--F:protein domain specific binding
GO:0016197--P:endosomal transport
GO:0008333--P:endosome to lysosome transport
GO:0007173--P:epidermal growth factor receptor signaling pathway
GO:0010324--P:membrane invagination
GO:0061024--P:membrane organization
GO:0008285--P:negative regulation of cell proliferation
GO:0042059--P:negative regulation of epidermal growth factor receptor signaling pathway
GO:0046426--P:negative regulation of JAK-STAT cascade
GO:1903543--P:positive regulation of exosomal secretion
GO:0010628--P:positive regulation of gene expression
GO:0006892--P:post-Golgi vesicle-mediated transport
GO:0072657--P:protein localization to membrane
GO:0006622--P:protein targeting to lysosome
GO:0043405--P:regulation of MAP kinase activity
GO:0042176--P:regulation of protein catabolic process
GO:0007165--P:signal transduction