dbPAF Protein Information


Tag Content
dbPAF ID dbPAF-0000761
Uniprot Accession O14802; RPC1_HUMAN; Q8IW34; Q8TCW5;
Genbank Protein ID NP_008986.2;
Genbank Nucleotide ID NM_007055.3;
Protein Name DNA-directed RNA polymerase III subunit RPC1
Protein Synonyms/Alias
Gene Name POLR3A
Gene Synonyms/Alias
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
Phosphorylation Sites
dbPAF PTMs: 20
PositionPeptidesSourceReferences ( PMIDs )
24HICFGMKSPEEMRQQPhosphoSitePlus22135298
53NQHAPLLYGVLDHRMPhosphoSitePlus22135298
434QMKRFLKYGNREKMAdbPTM 3.0;HPRD 9;PhosphoSitePlus;curated20068231; 16381945; 23193290; 18988627; 22135298; 22817900
525EALVLMGTKANLVTPdbPTM 3.0;PhosphoSitePlus;curated21406692; 16381945; 23193290; 22135298; 22817900
593PTILKPVTLWTGKQIPhosphoSitePlus22135298
596LKPVTLWTGKQIFSVPhosphoSitePlus22135298
681NEAADAMSRLARLAPPhosphoSitePlus22135298
714QGLLKAKYELLNAGYPhosphoSitePlus22135298
936KAVFPCPSEPALSKNdbPTM 3.0;HPRD 918767875; 16381945; 23193290; 18988627
941CPSEPALSKNELILTdbPTM 3.0;HPRD 918767875; 16381945; 23193290; 18988627
948SKNELILTTESIMKKPhosphoSitePlus22135298
949KNELILTTESIMKKSPhosphoSitePlus22135298
951ELILTTESIMKKSEFcurated19690332
977KKFIKGVSEKIKKTRPhosphoSitePlus;curated22135298; 25404012
1046QSIGEPGTQMTLKTFdbPTM 3.0;HPRD 9;PhosphoSitePlus18077418; 16381945; 23193290; 18988627; 22135298
1049GEPGTQMTLKTFHFAdbPTM 3.0;HPRD 9;PhosphoSitePlus18077418; 16381945; 23193290; 18988627; 22135298
1181CVTPRENSKSSMYYVdbPTM 3.0;HPRD 9;PhosphoSitePlus;curated20068231; 16381945; 23193290; 18988627; 22135298; 22817900
1247GVKGTRTTSNNTYEVPhosphoSitePlus22135298
1251TRTTSNNTYEVEKTLPhosphoSitePlus22135298
1304KGEVLGITRFGLAKMdbPTM 3.0;HPRD 9;PHOSIDA;PhosphoSitePlus;curated20068231; 16381945; 23193290; 18988627; 21081558; 22135298
Sequence
(Fasta)
MVKEQFRETD VAKKISHICF GMKSPEEMRQ QAHIQVVSKN LYSQDNQHAP LLYGVLDHRM 60
GTSEKDRPCE TCGKNLADCL GHYGYIDLEL PCFHVGYFRA VIGILQMICK TCCHIMLSQE 120
EKKQFLDYLK RPGLTYLQKR GLKKKISDKC RKKNICHHCG AFNGTVKKCG LLKIIHEKYK 180
TNKKVVDPIV SNFLQSFETA IEHNKEVEPL LGRAQENLNP LVVLNLFKRI PAEDVPLLLM 240
NPEAGKPSDL ILTRLLVPPL CIRPSVVSDL KSGTNEDDLT MKLTEIIFLN DVIKKHRISG 300
AKTQMIMEDW DFLQLQCALY INSELSGIPL NMAPKKWTRG FVQRLKGKQG RFRGNLSGKR 360
VDFSGRTVIS PDPNLRIDEV AVPVHVAKIL TFPEKVNKAN INFLRKLVQN GPEVHPGANF 420
IQQRHTQMKR FLKYGNREKM AQELKYGDIV ERHLIDGDVV LFNRQPSLHK LSIMAHLARV 480
KPHRTFRFNE CVCTPYNADF DGDEMNLHLP QTEEAKAEAL VLMGTKANLV TPRNGEPLIA 540
AIQDFLTGAY LLTLKDTFFD RAKACQIIAS ILVGKDEKIK VRLPPPTILK PVTLWTGKQI 600
FSVILRPSDD NPVRANLRTK GKQYCGKGED LCANDSYVTI QNSELMSGSM DKGTLGSGSK 660
NNIFYILLRD WGQNEAADAM SRLARLAPVY LSNRGFSIGI GDVTPGQGLL KAKYELLNAG 720
YKKCDEYIEA LNTGKLQQQP GCTAEETLEA LILKELSVIR DHAGSACLRE LDKSNSPLTM 780
ALCGSKGSFI NISQMIACVG QQAISGSRVP DGFENRSLPH FEKHSKLPAA KGFVANSFYS 840
GLTPTEFFFH TMAGREGLVD TAVKTAETGY MQRRLVKSLE DLCSQYDLTV RSSTGDIIQF 900
IYGGDGLDPA AMEGKDEPLE FKRVLDNIKA VFPCPSEPAL SKNELILTTE SIMKKSEFLC 960
CQDSFLQEIK KFIKGVSEKI KKTRDKYGIN DNGTTEPRVL YQLDRITPTQ VEKFLETCRD 1020
KYMRAQMEPG SAVGALCAQS IGEPGTQMTL KTFHFAGVAS MNITLGVPRI KEIINASKAI 1080
STPIITAQLD KDDDADYARL VKGRIEKTLL GEISEYIEEV FLPDDCFILV KLSLERIRLL 1140
RLEVNAETVR YSICTSKLRV KPGDVAVHGE AVVCVTPREN SKSSMYYVLQ FLKEDLPKVV 1200
VQGIPEVSRA VIHIDEQSGK EKYKLLVEGD NLRAVMATHG VKGTRTTSNN TYEVEKTLGI 1260
EAARTTIINE IQYTMVNHGM SIDRRHVMLL SDLMTYKGEV LGITRFGLAK MKESVLMLAS 1320
FEKTADHLFD AAYFGQKDSV CGVSECIIMG IPMNIGTGLF KLLHKADRDP NPPKRPLIFD 1380
TNEFHIPLVT 1391
Keyword

KW-0007--Acetylation
KW-0051--Antiviral defense
KW-0181--Complete proteome
KW-0225--Disease mutation
KW-0240--DNA-directed RNA polymerase
KW-0391--Immunity
KW-0399--Innate immunity
KW-1026--Leukodystrophy
KW-0460--Magnesium
KW-0479--Metal-binding
KW-0548--Nucleotidyltransferase
KW-0539--Nucleus
KW-0621--Polymorphism
KW-1185--Reference proteome
KW-0804--Transcription
KW-0808--Transferase
KW-0862--Zinc

Interpro

IPR000722--RNA_pol_asu
IPR006592--RNA_pol_N
IPR007080--RNA_pol_Rpb1_1
IPR007066--RNA_pol_Rpb1_3
IPR007083--RNA_pol_Rpb1_4
IPR007081--RNA_pol_Rpb1_5

PROSITE
Pfam

PF04997--RNA_pol_Rpb1_1
PF00623--RNA_pol_Rpb1_2
PF04983--RNA_pol_Rpb1_3
PF05000--RNA_pol_Rpb1_4
PF04998--RNA_pol_Rpb1_5

Gene Ontology

GO:0005829--C:cytosol
GO:0005666--C:DNA-directed RNA polymerase III complex
GO:0016020--C:membrane
GO:0005654--C:nucleoplasm
GO:0003682--F:chromatin binding
GO:0003677--F:DNA binding
GO:0003899--F:DNA-directed RNA polymerase activity
GO:0046872--F:metal ion binding
GO:0051607--P:defense response to virus
GO:0010467--P:gene expression
GO:0045087--P:innate immune response
GO:0032728--P:positive regulation of interferon-beta production
GO:1903955--P:positive regulation of protein targeting to mitochondrion
GO:0032481--P:positive regulation of type I interferon production
GO:1903146--P:regulation of mitophagy
GO:0006386--P:termination of RNA polymerase III transcription
GO:0006385--P:transcription elongation from RNA polymerase III promoter
GO:0006383--P:transcription from RNA polymerase III promoter
GO:0006351--P:transcription, DNA-templated