dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000750
Uniprot Accession O14777; NDC80_HUMAN; Q6PJX2;
Genbank Protein ID NP_006092.1;
Genbank Nucleotide ID NM_006101.2;
Protein Name Kinetochore protein NDC80 homolog
Protein Synonyms/Alias Highly expressed in cancer protein;Kinetochore protein Hec1;HsHec1;Kinetochore-associated protein 2;Retinoblastoma-associated protein HEC;
Gene Name NDC80
Gene Synonyms/Alias HEC;HEC1;KNTC2;
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)		 Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:9315664, PubMed:12351790, PubMed:14654001, PubMed:14699129, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:16732327). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).
Phosphorylation Sites
 dbPAF PTMs: 27 (View all)
PositionPeptidesSourceReferences ( PMIDs )
4----MKRSSVSSGGAcurated25849741
5---MKRSSVSSGGAGdbPTM 3.0;PhosphoSitePlus17129782; 16381945; 23193290; 22135298
15SGGAGRLSMQELRSQdbPTM 3.0;PhosphoSitePlus;curated17129782; 16381945; 23193290; 22135298; 25627689; 22817900
30DVNKQGLYTPQTKEKPhosphoSitePlus;curated22135298; 25627689
31VNKQGLYTPQTKEKPdbPTM 3.0;HPRD 9;PhosphoSitePlus;curated19007248; 16381945; 23193290; 18988627; 22135298; 22199227;
34QGLYTPQTKEKPTFGdbPTM 3.0;HPRD 9;curated19007248; 16381945; 23193290; 18988627; 22199227
39PQTKEKPTFGKLSINcurated22817900
44KPTFGKLSINKPTSEdbPTM 3.0;HPRD 9;PhosphoSitePlus;curated19007248; 16381945; 23193290; 17129782; 18988627; 22135298;
49KLSINKPTSERKVSLdbPTM 3.0;PhosphoSitePlus;curated17129782; 16381945; 23193290; 22135298; 25627689; 23898821;
50LSINKPTSERKVSLFdbPTM 3.0;HPRD 9;PhosphoSitePlus;curated19691289; 16381945; 23193290; 18988627; 22135298; 25627689;
55PTSERKVSLFGKRTSdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoSitePlus;SysPTM 2.0;curated16565220; 19007248; 16381945; 23193290; 17129782; 19691289;
61VSLFGKRTSGHGSRNPhosphoSitePlus;curated22135298; 25627689; 23898821
62SLFGKRTSGHGSRNSdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoSitePlus;SysPTM 2.0;curated16565220; 16381945; 23193290; 20068231; 18988627; 22135298;
66KRTSGHGSRNSQLGIdbPTM 3.0;HPRD 9;PhosphoSitePlus;curated20068231; 16381945; 23193290; 18988627; 22135298; 25627689;
69SGHGSRNSQLGIFSSdbPTM 3.0;HPRD 9;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;UniProt;curated19007248; 16381945; 23193290; 17129782; 19664994; 19691289;
75NSQLGIFSSSEKIKDcurated25627689; 22199227; 20873877
76SQLGIFSSSEKIKDPdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;curated16565220; 16381945; 23193290; 18988627; 19060867; 22135298;
77QLGIFSSSEKIKDPRdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;curated16565220; 16381945; 23193290; 20068231; 22067460; 19691289;
165LGYPFALSKSSMYTVdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoSitePlus;curated12386167; 16381945; 23193290; 18297113; 21832156; 18988627;
201IHTAMKESSPLFDDGcurated25627689
Sequence
(Fasta)		MKRSSVSSGG AGRLSMQELR SQDVNKQGLY TPQTKEKPTF GKLSINKPTS ERKVSLFGKR 60
TSGHGSRNSQ LGIFSSSEKI KDPRPLNDKA FIQQCIRQLC EFLTENGYAH NVSMKSLQAP 120
SVKDFLKIFT FLYGFLCPSY ELPDTKFEEE VPRIFKDLGY PFALSKSSMY TVGAPHTWPH 180
IVAALVWLID CIKIHTAMKE SSPLFDDGQP WGEETEDGIM HNKLFLDYTI KCYESFMSGA 240
DSFDEMNAEL QSKLKDLFNV DAFKLESLEA KNRALNEQIA RLEQEREKEP NRLESLRKLK 300
ASLQGDVQKY QAYMSNLESH SAILDQKLNG LNEEIARVEL ECETIKQENT RLQNIIDNQK 360
YSVADIERIN HERNELQQTI NKLTKDLEAE QQKLWNEELK YARGKEAIET QLAEYHKLAR 420
KLKLIPKGAE NSKGYDFEIK FNPEAGANCL VKYRAQVYVP LKELLNETEE EINKALNKKM 480
GLEDTLEQLN AMITESKRSV RTLKEEVQKL DDLYQQKIKE AEEEDEKCAS ELESLEKHKH 540
LLESTVNQGL SEAMNELDAV QREYQLVVQT TTEERRKVGN NLQRLLEMVA THVGSVEKHL 600
EEQIAKVDRE YEECMSEDLS ENIKEIRDKY EKKATLIKSS EE 643
Keyword

KW-0002--3D-structure
KW-0131--Cell cycle
KW-0132--Cell division
KW-0137--Centromere
KW-0158--Chromosome
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0995--Kinetochore
KW-0498--Mitosis
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-0621--Polymorphism
KW-1185--Reference proteome
Interpro

IPR005550--Kinetochore_Ndc80
PROSITE
Pfam

PF03801--Ndc80_HEC
Gene Ontology

GO:0000775--C:chromosome, centromeric region
GO:0000777--C:condensed chromosome kinetochore
GO:0000778--C:condensed nuclear chromosome kinetochore
GO:0000942--C:condensed nuclear chromosome outer kinetochore
GO:0005829--C:cytosol
GO:0000776--C:kinetochore
GO:0016020--C:membrane
GO:0031262--C:Ndc80 complex
GO:0005634--C:nucleus
GO:0042802--F:identical protein binding
GO:0005200--F:structural constituent of cytoskeleton
GO:0051315--P:attachment of mitotic spindle microtubules to kinetochore
GO:0008608--P:attachment of spindle microtubules to kinetochore
GO:0051301--P:cell division
GO:0007059--P:chromosome segregation
GO:0000132--P:establishment of mitotic spindle orientation
GO:0000278--P:mitotic cell cycle
GO:0007067--P:mitotic nuclear division
GO:0000070--P:mitotic sister chromatid segregation
GO:0007052--P:mitotic spindle organization
GO:0007264--P:small GTPase mediated signal transduction