dbPAF Protein Information


Tag Content
dbPAF ID dbPAF-0000738
Uniprot Accession O14746; TERT_HUMAN; O14783; Q2XS35; Q8N6C3; Q8NG38; Q8NG46;
Genbank Protein ID NP_001180305.1; NP_937983.2;
Genbank Nucleotide ID NM_001193376.1; NM_198253.2;
Protein Name Telomerase reverse transcriptase
Protein Synonyms/Alias HEST2;Telomerase catalytic subunit;Telomerase-associated protein 2;TP2;
Gene Name TERT
Gene Synonyms/Alias EST2;TCS1;TRT;
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)
Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.
Phosphorylation Sites
dbPAF PTMs: 13
PositionPeptidesSourceReferences ( PMIDs )
26REVLPLATFVRRLGPPhosphoSitePlus22135298
122FTTSVRSYLPNTVTDPhosphoSitePlus22135298
134VTDALRGSGAWGLLLPhosphoSitePlus22135298
227GARRRGGSASRSLPLdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoSitePlus;curated10224060; 16381945; 23193290; 22366458; 18988627; 22135298;
707QDPPPELYFVKVDVTdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoSitePlus;curated12808100; 18829466; 16381945; 23193290; 20211239; 18988627;
767KSHVSTLTDLQPYMRPhosphoSitePlus22135298
797VVIEQSSSLNEASSGdbPTM 3.0;Phospho.ELM 9.016565220; 16381945; 23193290
824AVRIRGKSYVQCQGIdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoSitePlus;curated10224060; 16381945; 23193290; 18988627; 22135298; 22817900
948EVQSDYSSYARTSIRPhosphoSitePlus22135298
949VQSDYSSYARTSIRAPhosphoSitePlus22135298
1101GSLRTAQTQLSRKLPdbPTM 3.016094384; 16381945; 23193290
1113KLPGTTLTALEAAANdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoSitePlus;curated16094384; 16381945; 23193290; 18988627; 22135298; 22817900
1125AANPALPSDFKTILDdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoSitePlus;curated16094384; 16381945; 23193290; 18988627; 22135298; 22817900
Sequence
(Fasta)
MPRAPRCRAV RSLLRSHYRE VLPLATFVRR LGPQGWRLVQ RGDPAAFRAL VAQCLVCVPW 60
DARPPPAAPS FRQVSCLKEL VARVLQRLCE RGAKNVLAFG FALLDGARGG PPEAFTTSVR 120
SYLPNTVTDA LRGSGAWGLL LRRVGDDVLV HLLARCALFV LVAPSCAYQV CGPPLYQLGA 180
ATQARPPPHA SGPRRRLGCE RAWNHSVREA GVPLGLPAPG ARRRGGSASR SLPLPKRPRR 240
GAAPEPERTP VGQGSWAHPG RTRGPSDRGF CVVSPARPAE EATSLEGALS GTRHSHPSVG 300
RQHHAGPPST SRPPRPWDTP CPPVYAETKH FLYSSGDKEQ LRPSFLLSSL RPSLTGARRL 360
VETIFLGSRP WMPGTPRRLP RLPQRYWQMR PLFLELLGNH AQCPYGVLLK THCPLRAAVT 420
PAAGVCAREK PQGSVAAPEE EDTDPRRLVQ LLRQHSSPWQ VYGFVRACLR RLVPPGLWGS 480
RHNERRFLRN TKKFISLGKH AKLSLQELTW KMSVRDCAWL RRSPGVGCVP AAEHRLREEI 540
LAKFLHWLMS VYVVELLRSF FYVTETTFQK NRLFFYRKSV WSKLQSIGIR QHLKRVQLRE 600
LSEAEVRQHR EARPALLTSR LRFIPKPDGL RPIVNMDYVV GARTFRREKR AERLTSRVKA 660
LFSVLNYERA RRPGLLGASV LGLDDIHRAW RTFVLRVRAQ DPPPELYFVK VDVTGAYDTI 720
PQDRLTEVIA SIIKPQNTYC VRRYAVVQKA AHGHVRKAFK SHVSTLTDLQ PYMRQFVAHL 780
QETSPLRDAV VIEQSSSLNE ASSGLFDVFL RFMCHHAVRI RGKSYVQCQG IPQGSILSTL 840
LCSLCYGDME NKLFAGIRRD GLLLRLVDDF LLVTPHLTHA KTFLRTLVRG VPEYGCVVNL 900
RKTVVNFPVE DEALGGTAFV QMPAHGLFPW CGLLLDTRTL EVQSDYSSYA RTSIRASLTF 960
NRGFKAGRNM RRKLFGVLRL KCHSLFLDLQ VNSLQTVCTN IYKILLLQAY RFHACVLQLP 1020
FHQQVWKNPT FFLRVISDTA SLCYSILKAK NAGMSLGAKG AAGPLPSEAV QWLCHQAFLL 1080
KLTRHRVTYV PLLGSLRTAQ TQLSRKLPGT TLTALEAAAN PALPSDFKTI LD 1133
Keyword

KW-0002--3D-structure
KW-0025--Alternative splicing
KW-0158--Chromosome
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0225--Disease mutation
KW-0238--DNA-binding
KW-1011--Dyskeratosis congenita
KW-0460--Magnesium
KW-0479--Metal-binding
KW-0548--Nucleotidyltransferase
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-0621--Polymorphism
KW-1185--Reference proteome
KW-0687--Ribonucleoprotein
KW-0695--RNA-directed DNA polymerase
KW-0779--Telomere
KW-0808--Transferase
KW-0832--Ubl conjugation

Interpro

IPR000477--RT_dom
IPR021891--Telomerase_RBD
IPR003545--Telomerase_RT

PROSITE

PS50878--RT_POL

Pfam

PF00078--RVT_1
PF12009--Telomerase_RBD

Gene Ontology

GO:0000781--C:chromosome, telomeric region
GO:0042645--C:mitochondrial nucleoid
GO:0000783--C:nuclear telomere cap complex
GO:0005730--C:nucleolus
GO:0005654--C:nucleoplasm
GO:0016605--C:PML body
GO:0031379--C:RNA-directed RNA polymerase complex
GO:0000333--C:telomerase catalytic core complex
GO:0005697--C:telomerase holoenzyme complex
GO:1990572--C:TERT-RMRP complex
GO:0003677--F:DNA binding
GO:0046872--F:metal ion binding
GO:0016779--F:nucleotidyltransferase activity
GO:0042803--F:protein homodimerization activity
GO:0003723--F:RNA binding
GO:0003964--F:RNA-directed DNA polymerase activity
GO:0003720--F:telomerase activity
GO:0042162--F:telomeric DNA binding
GO:0070034--F:telomeric RNA binding
GO:0003721--F:telomeric template RNA reverse transcriptase activity
GO:0001223--F:transcription coactivator binding
GO:0000049--F:tRNA binding
GO:0071456--P:cellular response to hypoxia
GO:0071897--P:DNA biosynthetic process
GO:0022616--P:DNA strand elongation
GO:0007005--P:mitochondrion organization
GO:2000773--P:negative regulation of cellular senescence
GO:2001240--P:negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
GO:0010629--P:negative regulation of gene expression
GO:1903704--P:negative regulation of production of siRNA involved in RNA interference
GO:0042635--P:positive regulation of hair cycle
GO:0051000--P:positive regulation of nitric-oxide synthase activity
GO:1902895--P:positive regulation of pri-miRNA transcription from RNA polymerase II promoter
GO:2000648--P:positive regulation of stem cell proliferation
GO:0030177--P:positive regulation of Wnt signaling pathway
GO:0030422--P:production of siRNA involved in RNA interference
GO:0090399--P:replicative senescence
GO:0032774--P:RNA biosynthetic process
GO:0006278--P:RNA-dependent DNA replication
GO:0000723--P:telomere maintenance
GO:0007004--P:telomere maintenance via telomerase
GO:0001172--P:transcription, RNA-templated