dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000737
Uniprot Accession O14745; NHRF1_HUMAN; B3KY21; O43552; Q86WQ5;
Genbank Protein ID NP_004243.1;
Genbank Nucleotide ID NM_004252.4;
Protein Name Na(+)/H(+) exchange regulatory cofactor NHE-RF1
Protein Synonyms/Alias
Gene Name SLC9A3R1
Gene Synonyms/Alias NHERF;NHERF1;
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)		 Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Involved in the regulation of phosphate reabsorption in the renal proximal tubules. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa.
Phosphorylation Sites
 dbPAF PTMs: 31 (View all)
PositionPeptidesSourceReferences ( PMIDs )
2------MSADAAAGAdbPTM 3.0;HPRD 9;PhosphoSitePlus;curated19664995; 21406692; 22115753; 16381945; 23193290; 19413330;
38EKGKLGQYIRLVEPGdbPTM 3.0;PhosphoSitePlus;curated20736484; 16381945; 23193290; 22135298; 22817900
46IRLVEPGSPAEKAGLdbPTM 3.0;HPRD 9;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;UniProt;curated18669648; 16381945; 23193290; 20068231; 21406692; 22115753;
77ETHQQVVSRIRAALNdbPTM 3.0;PhosphoSitePlus;curated17895247; 20571032; 16381945; 23193290; 22135298; 22817900
95LLVVDPETDEQLQKLdbPTM 3.0;PhosphoSitePlus;curated17895247; 20571032; 16381945; 23193290; 22135298; 22817900
156ELRPRLCTMKKGPSGPhosphoSitePlus22135298
162CTMKKGPSGYGFNLHdbPTM 3.0;Phospho.ELM 9.0;PhosphoSitePlus;UniProt;curated12881487; 16381945; 23193290; 17613530; 21406692; 22135298;
164MKKGPSGYGFNLHSDPhosphoSitePlus22135298
170GYGFNLHSDKSKPGQPhosphoSitePlus22135298
173FNLHSDKSKPGQFIRPhosphoSitePlus22135298
181KPGQFIRSVDPDSPAPhosphoSitePlus22135298
191PDSPAEASGLRAQDRPhosphoSitePlus22135298
217KQHGDVVSAIRAGGDdbPTM 3.0;PhosphoSitePlus;curated21406692; 16381945; 23193290; 22135298; 22817900
226IRAGGDETKLLVVDRcurated22817900
260GPLPVPFTNGEIQKEPhosphoSitePlus22135298
269GEIQKENSREALAEAdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PHOSIDA;PhosphoSitePlus;SysPTM 2.0;UniProt;curated17081983; 16381945; 23193290; 20363803; 21406692; 22115753;
280LAEAALESPRPALVRdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;UniProt;curated17081983; 17924679; 18088087; 18220336; 18669648; 16381945;
288PRPALVRSASSDTSEdbPTM 3.0;HPRD 9;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;curated19664995; 19690332; 20044836; 20068231; 20166139; 20736484;
290PALVRSASSDTSEELdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;UniProt;curated18088087; 16381945; 23193290; 18669648; 15302935; 10446210;
291ALVRSASSDTSEELNdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;UniProt;curated18088087; 16381945; 23193290; 19664995; 19690332; 20058876;
Sequence
(Fasta)		MSADAAAGAP LPRLCCLEKG PNGYGFHLHG EKGKLGQYIR LVEPGSPAEK AGLLAGDRLV 60
EVNGENVEKE THQQVVSRIR AALNAVRLLV VDPETDEQLQ KLGVQVREEL LRAQEAPGQA 120
EPPAAAEVQG AGNENEPREA DKSHPEQREL RPRLCTMKKG PSGYGFNLHS DKSKPGQFIR 180
SVDPDSPAEA SGLRAQDRIV EVNGVCMEGK QHGDVVSAIR AGGDETKLLV VDRETDEFFK 240
KCRVIPSQEH LNGPLPVPFT NGEIQKENSR EALAEAALES PRPALVRSAS SDTSEELNSQ 300
DSPPKQDSTA PSSTSSSDPI LDFNISLAMA KERAHQKRSS KRAPQMDWSK KNELFSNL 359
Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0025--Alternative splicing
KW-1003--Cell membrane
KW-0966--Cell projection
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0903--Direct protein sequencing
KW-0225--Disease mutation
KW-0472--Membrane
KW-0597--Phosphoprotein
KW-0621--Polymorphism
KW-1185--Reference proteome
KW-0677--Repeat
KW-0879--Wnt signaling pathway
Interpro

IPR015098--EBP50_C-term
IPR031199--NHERF-1
IPR017300--NHERF-1/NHERF-2
IPR001478--PDZ
PROSITE

PS50106--PDZ
Pfam

PF09007--EBP50_C
PF00595--PDZ
Gene Ontology

GO:0015629--C:actin cytoskeleton
GO:0016324--C:apical plasma membrane
GO:0031526--C:brush border membrane
GO:0071944--C:cell periphery
GO:0005813--C:centrosome
GO:0005737--C:cytoplasm
GO:0012505--C:endomembrane system
GO:0070062--C:extracellular exosome
GO:0030175--C:filopodium
GO:0043231--C:intracellular membrane-bounded organelle
GO:0016020--C:membrane
GO:0045121--C:membrane raft
GO:0005902--C:microvillus
GO:0031528--C:microvillus membrane
GO:0048471--C:perinuclear region of cytoplasm
GO:0005886--C:plasma membrane
GO:0001726--C:ruffle
GO:0097225--C:sperm midpiece
GO:0032420--C:stereocilium
GO:0032426--C:stereocilium bundle tip
GO:0031982--C:vesicle
GO:0031698--F:beta-2 adrenergic receptor binding
GO:0008013--F:beta-catenin binding
GO:0017081--F:chloride channel regulator activity
GO:0070851--F:growth factor receptor binding
GO:0030165--F:PDZ domain binding
GO:0019902--F:phosphatase binding
GO:0043621--F:protein self-association
GO:0005102--F:receptor binding
GO:0030036--P:actin cytoskeleton organization
GO:0007191--P:adenylate cyclase-activating dopamine receptor signaling pathway
GO:0060088--P:auditory receptor cell stereocilium organization
GO:0032782--P:bile acid secretion
GO:0030643--P:cellular phosphate ion homeostasis
GO:0045198--P:establishment of epithelial cell apical/basal polarity
GO:0051683--P:establishment of Golgi localization
GO:0040023--P:establishment of nucleus localization
GO:0090002--P:establishment of protein localization to plasma membrane
GO:0022612--P:gland morphogenesis
GO:0034635--P:glutathione transport
GO:0030033--P:microvillus assembly
GO:0035414--P:negative regulation of catenin import into nucleus
GO:0030336--P:negative regulation of cell migration
GO:0008285--P:negative regulation of cell proliferation
GO:0070373--P:negative regulation of ERK1 and ERK2 cascade
GO:0045930--P:negative regulation of mitotic cell cycle
GO:0014067--P:negative regulation of phosphatidylinositol 3-kinase signaling
GO:0010642--P:negative regulation of platelet-derived growth factor receptor signaling pathway
GO:0051898--P:negative regulation of protein kinase B signaling
GO:0032416--P:negative regulation of sodium:proton antiporter activity
GO:0060158--P:phospholipase C-activating dopamine receptor signaling pathway
GO:2001244--P:positive regulation of intrinsic apoptotic signaling pathway
GO:0006461--P:protein complex assembly
GO:0008360--P:regulation of cell shape
GO:0008361--P:regulation of cell size
GO:0044062--P:regulation of excretion
GO:0045859--P:regulation of protein kinase activity
GO:0032415--P:regulation of sodium:proton antiporter activity
GO:0070293--P:renal absorption
GO:0097291--P:renal phosphate ion absorption
GO:0003096--P:renal sodium ion transport
GO:0007605--P:sensory perception of sound
GO:0016055--P:Wnt signaling pathway