dbPAF Protein Information


Tag Content
dbPAF ID dbPAF-0000736
Uniprot Accession O14744; ANM5_HUMAN; A8MTP3; A8MZ91; B4DX49; B4DY30; B5BU10; D3DS33; E2QRE7; Q6IBR1; Q9UKH1;
Genbank Protein ID NP_001034708.1; NP_001269882.1; NP_001269884.1; NP_001269885.1; NP_006100.2;
Genbank Nucleotide ID NM_001039619.2; NM_001282953.1; NM_001282955.1; NM_001282956.1; NM_006109.4;
Protein Name Protein arginine N-methyltransferase 5
Protein Synonyms/Alias 72 kDa ICln-binding protein;Histone-arginine N-methyltransferase PRMT5;
Gene Name PRMT5
Gene Synonyms/Alias HRMT1L5;IBP72;JBP1;SKB1;
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)
Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA (PubMed:10531356, PubMed:11152681, PubMed:11747828, PubMed:12411503, PubMed:15737618, PubMed:17709427, PubMed:20159986, PubMed:20810653, PubMed:21258366, PubMed:21917714, PubMed:22269951). Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles (PubMed:12411503, PubMed:11747828, PubMed:17709427). Methylates SUPT5H (PubMed:12718890). Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage (By similarity). Methylates RPS10. Attenuates EGF signaling through the MAPK1/MAPK3 pathway acting at 2 levels. First, monomethylates EGFR; this enhances EGFR 'Tyr-1197' phosphorylation and PTPN6 recruitment, eventually leading to reduced SOS1 phosphorylation (PubMed:21917714, PubMed:21258366). Second, methylates RAF1 and probably BRAF, hence destabilizing these 2 signaling proteins and reducing their catalytic activity (PubMed:21917714). Required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation. Methylates HOXA9 (PubMed:22269951). Methylates and regulates SRGAP2 which is involved in cell migration and differentiation (PubMed:20810653). Acts as a transcriptional corepressor in CRY1-mediated repression of the core circadian component PER1 by regulating the H4R3 dimethylation at the PER1 promoter (By similarity). Methylates GM130/GOLGA2, regulating Golgi ribbon formation (PubMed:20421892).
Phosphorylation Sites
dbPAF PTMs: 12
PositionPeptidesSourceReferences ( PMIDs )
12AVGGAGGSRVSSGRDdbPTM 3.0;HPRD 9;PhosphoSitePlus19651622; 16381945; 23193290; 18988627; 22135298
74TRSDLLLSGRDWNTLPhosphoSitePlus22135298
280SEKEFCSYLQYLEYLdbPTM 3.0;PhosphoSitePlus;curated18083107; 16381945; 23193290; 22135298; 22817900
283EFCSYLQYLEYLSQNdbPTM 3.0;PhosphoSitePlus;curated18083107; 16381945; 23193290; 22135298; 22817900
286SYLQYLEYLSQNRPPdbPTM 3.0;PhosphoSitePlus;curated18083107; 16381945; 23193290; 22135298; 22817900
297NRPPPNAYELFAKGYdbPTM 3.0;PhosphoSitePlus21316606; 16381945; 23193290; 22135298
304YELFAKGYEDYLQSPdbPTM 3.0;PhosphoSitePlus21316606; 16381945; 23193290; 22135298
307FAKGYEDYLQSPLQPdbPTM 3.0;PhosphoSitePlus21316606; 16381945; 23193290; 22135298
310GYEDYLQSPLQPLMDPhosphoSitePlus;curated22135298; 25627689
335EKDPIKYSQYQQAIYcurated22817900
337DPIKYSQYQQAIYKCcurated26074081
342SQYQQAIYKCLLDRVPhosphoSitePlus;curated22135298; 22817900; 26074081
Sequence
(Fasta)
MAAMAVGGAG GSRVSSGRDL NCVPEIADTL GAVAKQGFDF LCMPVFHPRF KREFIQEPAK 60
NRPGPQTRSD LLLSGRDWNT LIVGKLSPWI RPDSKVEKIR RNSEAAMLQE LNFGAYLGLP 120
AFLLPLNQED NTNLARVLTN HIHTGHHSSM FWMRVPLVAP EDLRDDIIEN APTTHTEEYS 180
GEEKTWMWWH NFRTLCDYSK RIAVALEIGA DLPSNHVIDR WLGEPIKAAI LPTSIFLTNK 240
KGFPVLSKMH QRLIFRLLKL EVQFIITGTN HHSEKEFCSY LQYLEYLSQN RPPPNAYELF 300
AKGYEDYLQS PLQPLMDNLE SQTYEVFEKD PIKYSQYQQA IYKCLLDRVP EEEKDTNVQV 360
LMVLGAGRGP LVNASLRAAK QADRRIKLYA VEKNPNAVVT LENWQFEEWG SQVTVVSSDM 420
REWVAPEKAD IIVSELLGSF ADNELSPECL DGAQHFLKDD GVSIPGEYTS FLAPISSSKL 480
YNEVRACREK DRDPEAQFEM PYVVRLHNFH QLSAPQPCFT FSHPNRDPMI DNNRYCTLEF 540
PVEVNTVLHG FAGYFETVLY QDITLSIRPE THSPGMFSWF PILFPIKQPI TVREGQTICV 600
RFWRCSNSKK VWYEWAVTAP VCSAIHNPTG RSYTIGL 638
Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0025--Alternative splicing
KW-0090--Biological rhythms
KW-0156--Chromatin regulator
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0903--Direct protein sequencing
KW-0333--Golgi apparatus
KW-0489--Methyltransferase
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0678--Repressor
KW-0949--S-adenosyl-L-methionine
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0808--Transferase

Interpro

IPR025799--Arg_MeTrfase
IPR007857--Arg_MeTrfase_PRMT5
IPR029063--SAM-dependent_MTases

PROSITE

PS51678--SAM_MT_PRMT

Pfam

PF05185--PRMT5

Gene Ontology

GO:0005737--C:cytoplasm
GO:0005829--C:cytosol
GO:0005794--C:Golgi apparatus
GO:0035097--C:histone methyltransferase complex
GO:0034709--C:methylosome
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0001046--F:core promoter sequence-specific DNA binding
GO:0008469--F:histone-arginine N-methyltransferase activity
GO:0008168--F:methyltransferase activity
GO:0046982--F:protein heterodimerization activity
GO:0016274--F:protein-arginine N-methyltransferase activity
GO:0035243--F:protein-arginine omega-N symmetric methyltransferase activity
GO:0043021--F:ribonucleoprotein complex binding
GO:0003714--F:transcription corepressor activity
GO:0008283--P:cell proliferation
GO:0006325--P:chromatin organization
GO:0032922--P:circadian regulation of gene expression
GO:0042118--P:endothelial cell activation
GO:0010467--P:gene expression
GO:0090161--P:Golgi ribbon formation
GO:0034969--P:histone arginine methylation
GO:0043985--P:histone H4-R3 methylation
GO:0097421--P:liver regeneration
GO:0034660--P:ncRNA metabolic process
GO:0045596--P:negative regulation of cell differentiation
GO:1903507--P:negative regulation of nucleic acid-templated transcription
GO:0018216--P:peptidyl-arginine methylation
GO:0019918--P:peptidyl-arginine methylation, to symmetrical-dimethyl arginine
GO:0035246--P:peptidyl-arginine N-methylation
GO:0048714--P:positive regulation of oligodendrocyte differentiation
GO:0044030--P:regulation of DNA methylation
GO:0070372--P:regulation of ERK1 and ERK2 cascade
GO:0007088--P:regulation of mitotic nuclear division
GO:0006355--P:regulation of transcription, DNA-templated
GO:0000387--P:spliceosomal snRNP assembly
GO:0006351--P:transcription, DNA-templated