dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000733
Uniprot Accession O14733; MP2K7_HUMAN; B2R9S5; D6W659; O14648; O14816; O60452; O60453; Q1PG43; Q8IY10;
Genbank Protein ID NP_001284484.1; NP_001284485.1; NP_660186.1;
Genbank Nucleotide ID NM_001297555.1; NM_001297556.1; NM_145185.3;
Protein Name Dual specificity mitogen-activated protein kinase kinase 7
Protein Synonyms/Alias
Gene Name MAP2K7
Gene Synonyms/Alias JNKK2;MEK7;MKK7;PRKMK7;SKK4;
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)		 Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.
Phosphorylation Sites
 dbPAF PTMs: 24 (View all)
PositionPeptidesSourceReferences ( PMIDs )
35IDLNLDISPQRPRPTdbPTM 3.0;HPRD 9;PhosphoPep 2.0;PhosphoSitePlus;curated20068231; 16381945; 23193290; 18988627; 19060867; 22135298;
42SPQRPRPTLQLPLANcurated20873877
53PLANDGGSRSPSSEScurated20873877
55ANDGGSRSPSSESSPcurated20873877; 25849741
57DGGSRSPSSESSPQHcurated20873877; 25849741
58GGSRSPSSESSPQHPcurated20873877; 26074081
60SRSPSSESSPQHPTPcurated23898821; 20873877; 25849741
61RSPSSESSPQHPTPPPhosphoSitePlus;curated22135298; 23911959; 23917254; 20873877; 25849741
66ESSPQHPTPPARPRHPhosphoSitePlus;curated22135298; 23917254; 25849741
79RHMLGLPSTLFTPRSPhosphoSitePlus22135298
83GLPSTLFTPRSMESIPhosphoSitePlus;curated22135298; 21857030
86STLFTPRSMESIEIDdbPTM 3.0;HPRD 9;PhosphoSitePlus;curated20068231; 16381945; 23193290; 18988627; 22135298
89FTPRSMESIEIDQKLdbPTM 3.0;HPRD 9;PhosphoSitePlus;curated20068231; 16381945; 23193290; 18988627; 22135298
142WKMRFRKTGHVIAVKPhosphoSitePlus22135298
271ISGRLVDSKAKTRSAdbPTM 3.0;Phospho.ELM 9.0;PhosphoSitePlus9312068; 16381945; 23193290; 22135298
275LVDSKAKTRSAGCAAdbPTM 3.0;Phospho.ELM 9.0;PhosphoSitePlus9312068; 16381945; 23193290; 22135298
277DSKAKTRSAGCAAYMPhosphoSitePlus22135298
283RSAGCAAYMAPERIDPhosphoSitePlus22135298
378YNKLLEHSFIKRYETPhosphoSitePlus;curated22135298; 20873877
401FKDVMAKTESPRTSGdbPTM 3.0;PhosphoSitePlus;curated22115753; 16381945; 23193290; 22135298; 23090842
Sequence
(Fasta)		MAASSLEQKL SRLEAKLKQE NREARRRIDL NLDISPQRPR PTLQLPLAND GGSRSPSSES 60
SPQHPTPPAR PRHMLGLPST LFTPRSMESI EIDQKLQEIM KQTGYLTIGG QRYQAEINDL 120
ENLGEMGSGT CGQVWKMRFR KTGHVIAVKQ MRRSGNKEEN KRILMDLDVV LKSHDCPYIV 180
QCFGTFITNT DVFIAMELMG TCAEKLKKRM QGPIPERILG KMTVAIVKAL YYLKEKHGVI 240
HRDVKPSNIL LDERGQIKLC DFGISGRLVD SKAKTRSAGC AAYMAPERID PPDPTKPDYD 300
IRADVWSLGI SLVELATGQF PYKNCKTDFE VLTKVLQEEP PLLPGHMGFS GDFQSFVKDC 360
LTKDHRKRPK YNKLLEHSFI KRYETLEVDV ASWFKDVMAK TESPRTSGVL SQPHLPFFR 420
Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0025--Alternative splicing
KW-0053--Apoptosis
KW-0067--ATP-binding
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0418--Kinase
KW-0460--Magnesium
KW-0479--Metal-binding
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-0621--Polymorphism
KW-1185--Reference proteome
KW-0723--Serine/threonine-protein kinase
KW-0346--Stress response
KW-0808--Transferase
KW-0829--Tyrosine-protein kinase
Interpro

IPR011009--Kinase-like_dom
IPR000719--Prot_kinase_dom
IPR002290--Ser/Thr_dual-sp_kinase
IPR008271--Ser/Thr_kinase_AS
PROSITE

PS50011--PROTEIN_KINASE_DOM
PS00108--PROTEIN_KINASE_ST
Pfam

PF00069--Pkinase
Gene Ontology

GO:0005737--C:cytoplasm
GO:0005829--C:cytosol
GO:0005622--C:intracellular
GO:0005634--C:nucleus
GO:0005524--F:ATP binding
GO:0019899--F:enzyme binding
GO:0008545--F:JUN kinase kinase activity
GO:0000287--F:magnesium ion binding
GO:0004708--F:MAP kinase kinase activity
GO:0019901--F:protein kinase binding
GO:0019903--F:protein phosphatase binding
GO:0004674--F:protein serine/threonine kinase activity
GO:0004713--F:protein tyrosine kinase activity
GO:0007257--P:activation of JUN kinase activity
GO:0006915--P:apoptotic process
GO:0038095--P:Fc-epsilon receptor signaling pathway
GO:0045087--P:innate immune response
GO:0007254--P:JNK cascade
GO:0002755--P:MyD88-dependent toll-like receptor signaling pathway
GO:0002756--P:MyD88-independent toll-like receptor signaling pathway
GO:0009408--P:response to heat
GO:0006970--P:response to osmotic stress
GO:0034612--P:response to tumor necrosis factor
GO:0009411--P:response to UV
GO:0007165--P:signal transduction
GO:0051403--P:stress-activated MAPK cascade
GO:0034166--P:toll-like receptor 10 signaling pathway
GO:0034134--P:toll-like receptor 2 signaling pathway
GO:0034138--P:toll-like receptor 3 signaling pathway
GO:0034142--P:toll-like receptor 4 signaling pathway
GO:0034146--P:toll-like receptor 5 signaling pathway
GO:0034162--P:toll-like receptor 9 signaling pathway
GO:0002224--P:toll-like receptor signaling pathway
GO:0038123--P:toll-like receptor TLR1:TLR2 signaling pathway
GO:0038124--P:toll-like receptor TLR6:TLR2 signaling pathway
GO:0035666--P:TRIF-dependent toll-like receptor signaling pathway