dbPAF Protein Information


Tag Content
dbPAF ID dbPAF-0000661
Uniprot Accession O14503; BHE40_HUMAN; Q96TD3;
Genbank Protein ID NP_003661.1;
Genbank Nucleotide ID NM_003670.2;
Protein Name Class E basic helix-loop-helix protein 40
Protein Synonyms/Alias
Gene Name BHLHE40
Gene Synonyms/Alias BHLHB2;DEC1;SHARP2;STRA13;
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)
Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA and NR1H3/LXRA transactivation activity. May be involved in the regulation of chondrocyte differentiation via the cAMP pathway.
Phosphorylation Sites
dbPAF PTMs: 10
PositionPeptidesSourceReferences ( PMIDs )
37PAHMYQVYKSRRGIKdbPTM 3.0;PhosphoSitePlus;curated18083107; 16381945; 23193290; 22135298; 22817900
226CVPVIQRTFAHSSGEcurated23090842
230IQRTFAHSSGEQSGScurated23090842
231QRTFAHSSGEQSGSDcurated23090842
235AHSSGEQSGSDTDTDPhosphoSitePlus;curated22135298; 23090842
237SSGEQSGSDTDTDSGcurated23090842
239GEQSGSDTDTDSGYGcurated23090842
243GSDTDTDSGYGGESEPhosphoSitePlus;curated22135298; 23909892
245DTDTDSGYGGESEKGcurated23090842
383LMPQRLPSPLPAHPScurated25850435; 23898821
Sequence
(Fasta)
MERIPSAQPP PACLPKAPGL EHGDLPGMYP AHMYQVYKSR RGIKRSEDSK ETYKLPHRLI 60
EKKRRDRINE CIAQLKDLLP EHLKLTTLGH LEKAVVLELT LKHVKALTNL IDQQQQKIIA 120
LQSGLQAGEL SGRNVETGQE MFCSGFQTCA REVLQYLAKH ENTRDLKSSQ LVTHLHRVVS 180
ELLQGGTSRK PSDPAPKVMD FKEKPSSPAK GSEGPGKNCV PVIQRTFAHS SGEQSGSDTD 240
TDSGYGGESE KGDLRSEQPC FKSDHGRRFT MGERIGAIKQ ESEEPPTKKN RMQLSDDEGH 300
FTSSDLISSP FLGPHPHQPP FCLPFYLIPP SATAYLPMLE KCWYPTSVPV LYPGLNASAA 360
ALSSFMNPDK ISAPLLMPQR LPSPLPAHPS VDSSVLLQAL KPIPPLNLET KD 413
Keyword

KW-0090--Biological rhythms
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0238--DNA-binding
KW-1017--Isopeptide bond
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0678--Repressor
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0832--Ubl conjugation

Interpro

IPR011598--bHLH_dom
IPR003650--Orange_dom

PROSITE

PS50888--BHLH
PS51054--ORANGE

Pfam

PF07527--Hairy_orange
PF00010--HLH

Gene Ontology

GO:0005794--C:Golgi apparatus
GO:0005730--C:nucleolus
GO:0005634--C:nucleus
GO:0043425--F:bHLH transcription factor binding
GO:0070888--F:E-box binding
GO:0043426--F:MRF binding
GO:0019904--F:protein domain specific binding
GO:0046982--F:protein heterodimerization activity
GO:0042803--F:protein homodimerization activity
GO:0001102--F:RNA polymerase II activating transcription factor binding
GO:0000981--F:RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0003714--F:transcription corepressor activity
GO:0003705--F:transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
GO:0003700--F:transcription factor activity, sequence-specific DNA binding
GO:0001078--F:transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
GO:0001191--F:transcriptional repressor activity, RNA polymerase II transcription factor binding
GO:0032922--P:circadian regulation of gene expression
GO:0007623--P:circadian rhythm
GO:0043153--P:entrainment of circadian clock by photoperiod
GO:0043433--P:negative regulation of sequence-specific DNA binding transcription factor activity
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter
GO:0045892--P:negative regulation of transcription, DNA-templated
GO:0006355--P:regulation of transcription, DNA-templated
GO:0006351--P:transcription, DNA-templated