dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000628
Uniprot Accession O14299; WIS4_SCHPO; P87062; Q92384;
Genbank Protein ID NP_593557.1;
Genbank Nucleotide ID NM_001018990.2;
Protein Name MAP kinase kinase kinase wis4
Protein Synonyms/Alias MAP kinase kinase kinase wak1;MAP kinase kinase kinase wik1;
Gene Name wis4
Gene Synonyms/Alias wak1;wik1;SPAC9G1.02;
Organism Schizosaccharomyces pombe (strain 972 / ATCC 24843)(Fission yeast)
NCBI Taxa ID 284812
Functional Description
(View all)		 Involved in a signal transduction pathway that is activated in under conditions of heat shock, oxidative stress or limited nutrition. Unlike win1, it is not activated by changes in the osmolarity of the extracellular environment. Activates the wis1 MAP kinase kinase by phosphorylation.
Phosphorylation Sites
 dbPAF PTMs: 4
PositionPeptidesSourceReferences ( PMIDs )
42YVRQGFASPHQSLMDcurated22817900
71FVSHIPLTPSHSGQScurated22817900
75IPLTPSHSGQSEKLMcurated22817900
111ANLTRNSSFNHQHYScurated22817900
Sequence
(Fasta)		MGLEHTFYPA EDRFEPLLEH SEPVNFVPKE NAKSYVRQGF ASPHQSLMDN LVDSTESTKR 60
SENFVSHIPL TPSHSGQSEK LMSTRTSHSP YISPTMSYTN HSPANLTRNS SFNHQHYSTT 120
LRSPPSMRGR GIDVNSSHYP HISRPRTSSD SQKMYTRAPV DYYYIQENPY FNNIDQDSIS 180
DKSLPSTNQS LHHSEEDTES DNDFSESIHP EFDIDVFYKV SNILYDESDL QDPEKRERLE 240
WHSMLSSVLK GDVMQTEKRR LRLTEPDGHS GTYISEVWLG LQAWLHGRLN ADQAEVIRKS 300
REGVEPVLRE VIDFQIQDEE TTKPPLEQVT EILEKVEQCK QFYISSREME ENVPLSASKE 360
FNYKLNALIS WSNVMESIQV ETLVLQKWVG NDEFDLTMRT PQFNYDGVEN TSSFVERIFR 420
QSGLQRTFEQ RTLTTLNRII HQAKQTISEN AQAFEEMKLP TYEDKLLPLV RFPIKLLEEA 480
LRLRLAYAKK IKGPNFLIVD SMLDDFKIAL SVAVRIKREY IKIASPSPGW SLPTNVDEDY 540
DNVLLDSLKF YFKLLTLKLS SGNKNLYFKE IDFLENEWAF LNEHIYWING GDIHMAGQFS 600
YLSNSLLLNV HRYVESHLNG PTERTAASLT NWYSTLLKNT QIRFRKILRF SETLNSRFEN 660
ASDFVISEGH LPDLVNRLST TGHFLAYTAN LERDGVFVIA DHTLSENPEA LKALLFSKDI 720
SNLETIQQNC SYVLILCPVH PIVWKGRIEK VDVPDFSVDL KTNRVRIIAS NKREHLQAAK 780
SVFQSISGDL VTLAVECRSS ITRVYKEFIR LSKLCMRISS TVVDCVSAVR EACSGVNCHD 840
LIYHVFSFAA EFGQRILRFL SFDSYWQTKL KRKITSLAVE WISFICDECD LMDRKTFRWG 900
VGALEFLMLM IRGNNILLID DAMFLKIREK VGKSMAFLLT HFDVLGAKSK VAAKLQREST 960
EVSSSPRLTS FGDVEEEALS IQLLQKETML RIDELEIERN NTLLERLAIG HVLDDSVFRN 1020
RDFIKLASSF SNITIRWQQG HFVRSGMFGD VYTGVNMETG DLLAVKEIKL QDSRTFRSTV 1080
DQIHNEMTVL ERLNHPNVVT YYGVEVHREK VYIFMEFCQG GSLADLLAHG RIEDENVLKV 1140
YVVQLLEGLA YIHSQHILHR DIKPANILLD HRGMIKYSDF GSALYVSPPT DPEVRYEDIQ 1200
PELQHLAGTP MYMAPEIILG TKKGDFGAMD IWSLGCVILE MMTGSTPWSE MDNEWAIMYH 1260
VAAMHTPSIP QNEKISSLAR DFIEQCFERD PEQRPRAVDL LTHPWITDFR KKTIITMPPA 1320
TITKKTSLSH TITEEKTAQL LAGRHDDSKA ETDSLAASYK EESALPVASN VGLRQPNELR 1380
IDSINLPPAI VTPDTINYSV D 1402
Keyword

KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0418--Kinase
KW-0547--Nucleotide-binding
KW-1185--Reference proteome
KW-0723--Serine/threonine-protein kinase
KW-0808--Transferase
Interpro

IPR011009--Kinase-like_dom
IPR017240--MAPKKK_Ssk2/Ssk22
IPR000719--Prot_kinase_dom
IPR002290--Ser/Thr_dual-sp_kinase
IPR008271--Ser/Thr_kinase_AS
PROSITE

PS50011--PROTEIN_KINASE_DOM
PS00108--PROTEIN_KINASE_ST
Pfam

PF00069--Pkinase
Gene Ontology

GO:0005737--C:cytoplasm
GO:0005829--C:cytosol
GO:0005524--F:ATP binding
GO:0004709--F:MAP kinase kinase kinase activity
GO:0000186--P:activation of MAPKK activity
GO:0000165--P:MAPK cascade
GO:0006468--P:protein phosphorylation
GO:0042981--P:regulation of apoptotic process
GO:0051403--P:stress-activated MAPK cascade
GO:0031098--P:stress-activated protein kinase signaling cascade