| Tag |
Content |
| dbPAF ID |
dbPAF-0000533 |
| Uniprot Accession |
O13914; SYA_SCHPO;
|
| Genbank Protein ID |
NP_593640.1;
|
| Genbank Nucleotide ID |
NM_001019071.2;
|
| Protein Name |
Alanine--tRNA ligase |
| Protein Synonyms/Alias |
Alanyl-tRNA synthetase;AlaRS; |
| Gene Name |
ala1 |
| Gene Synonyms/Alias |
ars1;SPAC23C11.09; |
| Organism |
Schizosaccharomyces pombe (strain 972 / ATCC 24843)(Fission yeast) |
| NCBI Taxa ID |
284812 |
Functional Description
(View all) |
Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.Functional Description
Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain. |
Phosphorylation Sites
|
dbPAF PTMs: 2 |
Sequence
(Fasta) | MTAESEVVNW PANEIRRTFL KYFEDHGHTI VPSSSVIPYD DPTLLFANAG MNQFKPIFLG 60
TVDPSSDFAK LKRACDSQKC IRAGGKHNDL EDVGKDNYHH TMFEMLGNWS FGDYFKKEAI 120
AMAWDLLTNV YGLKKDQLYV TYFGGHEESG LEPDLEARQL WLDIGIDESR VIPGSLKDNF 180
WEMGDQGPCG PCSEIHYDRI GNRTVPELVN MDDPNVLEIW NIVFIQFNRE KDGSLRPLPN 240
RHVDTGMGFE RLVSVIQNKT SNYDTDVFSP IFAKIQELTN ARPYTGKMGD EDVDGIDTAY 300
RVVADHVRTL TFAISDGGVP NNEGRGYVLR RILRRGARYV RKKFGVPIGN FFSRLSLTVV 360
EQMGDFFPEL KRKVDDVREL LDEEEESFSR TLDRGEKMFE QYAAAAKKTP SKTLQGNDVW 420
RLYETYGFPV DLTHLMAEEA GIKIDEPGFE AAQARSKEIS KQASKGGSSG DDLLVLDVHA 480
LGALSKMDDI PETDDVFKHN SVSLKSVIKG IYHKGGFQKS TEGFNSGEQL GLLLDRTNFY 540
AEQGGQEYDT GHIVIDGVAD FRVTNVQVYA GYVLHTGFLE YGNLTVNDSV VCEYDEIRRW 600
HLMNNHTVTH ILNLALRNTL GDGIDQRGSL VSQEKLRFDF SYKSSIPIDK LLLVENYCNN 660
VIQDNLSVYS KEVALSKAKE INGLRAVFGE VYPDPVRVVC IGVDIDTLLQ EPKKPDWTKY 720
SIEFCGGTHC DKSGEIKDFV ILEENGIAKG IRRIVAVTST EANRVSRLAN EFDARIAKLE 780
KMPFSPAKEA ELKKISVDLS KLVVAAVRKH AMKERIAKIT KQVQEHVKAI NAAEQKEVVN 840
VVTEYFKENP DMSFVVAKVP ISANPKALSF ALTYAKKNLK DKSIYLLASD DTKVAHACLV 900
SPEAMKKLTP QEWSQKVCHS IGGRSGGKGD TCQGVGDKPL SIDVAVEEAI EFFQGKLTI
960
Fasta Sequence
>O13914|ala1|Schizosaccharomyces pombe (strain 972 / ATCC 24843)(Fission yeast)
MTAESEVVNWPANEIRRTFLKYFEDHGHTIVPSSSVIPYDDPTLLFANAGMNQFKPIFLGTVDPSSDFAKLKRACDSQKCIRAGGKHNDLEDVGKDNYHHTMFEMLGNWSFGDYFKKEAIAMAWDLLTNVYGLKKDQLYVTYFGGHEESGLEPDLEARQLWLDIGIDESRVIPGSLKDNFWEMGDQGPCGPCSEIHYDRIGNRTVPELVNMDDPNVLEIWNIVFIQFNREKDGSLRPLPNRHVDTGMGFERLVSVIQNKTSNYDTDVFSPIFAKIQELTNARPYTGKMGDEDVDGIDTAYRVVADHVRTLTFAISDGGVPNNEGRGYVLRRILRRGARYVRKKFGVPIGNFFSRLSLTVVEQMGDFFPELKRKVDDVRELLDEEEESFSRTLDRGEKMFEQYAAAAKKTPSKTLQGNDVWRLYETYGFPVDLTHLMAEEAGIKIDEPGFEAAQARSKEISKQASKGGSSGDDLLVLDVHALGALSKMDDIPETDDVFKHNSVSLKSVIKGIYHKGGFQKSTEGFNSGEQLGLLLDRTNFYAEQGGQEYDTGHIVIDGVADFRVTNVQVYAGYVLHTGFLEYGNLTVNDSVVCEYDEIRRWHLMNNHTVTHILNLALRNTLGDGIDQRGSLVSQEKLRFDFSYKSSIPIDKLLLVENYCNNVIQDNLSVYSKEVALSKAKEINGLRAVFGEVYPDPVRVVCIGVDIDTLLQEPKKPDWTKYSIEFCGGTHCDKSGEIKDFVILEENGIAKGIRRIVAVTSTEANRVSRLANEFDARIAKLEKMPFSPAKEAELKKISVDLSKLVVAAVRKHAMKERIAKITKQVQEHVKAINAAEQKEVVNVVTEYFKENPDMSFVVAKVPISANPKALSFALTYAKKNLKDKSIYLLASDDTKVAHACLVSPEAMKKLTPQEWSQKVCHSIGGRSGGKGDTCQGVGDKPLSIDVAVEEAIEFFQGKLTI
|
| Keyword |
KW-0030--Aminoacyl-tRNA synthetase
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0436--Ligase
KW-0479--Metal-binding
KW-0496--Mitochondrion
KW-0547--Nucleotide-binding
KW-0597--Phosphoprotein
KW-0648--Protein biosynthesis
KW-1185--Reference proteome
KW-0694--RNA-binding
KW-0820--tRNA-binding
KW-0862--Zinc
|
| Interpro |
IPR002318--Ala-tRNA-lgiase_IIc
IPR018162--Ala-tRNA-ligase_IIc_anticod-bd
IPR018165--Ala-tRNA-synth_IIc_core
IPR018164--Ala-tRNA-synth_IIc_N
IPR023033--Ala_tRNA_ligase_euk/bac
IPR003156--DHHA1_dom
IPR018163--Thr/Ala-tRNA-synth_IIc_edit
IPR009000--Transl_B-barrel
IPR012947--tRNA_SAD
|
| PROSITE |
PS50860--AA_TRNA_LIGASE_II_ALA
|
| Pfam |
PF02272--DHHA1
PF01411--tRNA-synt_2c
PF07973--tRNA_SAD
|
| Gene Ontology |
GO:0005737--C:cytoplasm
GO:0005829--C:cytosol
GO:0005739--C:mitochondrion
GO:0004813--F:alanine-tRNA ligase activity
GO:0005524--F:ATP binding
GO:0046872--F:metal ion binding
GO:0000049--F:tRNA binding
GO:1990762--P:cytoplasmic alanyl-tRNA aminoacylation
GO:0070143--P:mitochondrial alanyl-tRNA aminoacylation
|
