dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000522
Uniprot Accession O13870; NOT3_SCHPO;
Genbank Protein ID NP_594789.2;
Genbank Nucleotide ID NM_001020217.2;
Protein Name General negative regulator of transcription subunit 3
Protein Synonyms/Alias
Gene Name not3
Gene Synonyms/Alias SPAC1B3.05;
Organism Schizosaccharomyces pombe (strain 972 / ATCC 24843)(Fission yeast)
NCBI Taxa ID 284812
Functional Description
(View all)		 Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery (By similarity).
Phosphorylation Sites
 dbPAF PTMs: 11
PositionPeptidesSourceReferences ( PMIDs )
248RSGRSSSSSHSPSPSdbPTM 3.0;UniProt18257517; 16381945; 23193290
249SGRSSSSSHSPSPSAdbPTM 3.0;UniProt18257517; 16381945; 23193290
251RSSSSSHSPSPSASSdbPTM 3.0;UniProt18257517; 16381945; 23193290
253SSSSHSPSPSASSSSdbPTM 3.0;UniProt18257517; 16381945; 23193290
366VSNSKVASPETPNATdbPTM 3.0;UniProt;curated18257517; 16381945; 23193290; 22817900
369SKVASPETPNATHTAdbPTM 3.0;UniProt;curated18257517; 16381945; 23193290; 22817900
433KWDSLGHTASPKMQTdbPTM 3.0;UniProt18257517; 16381945; 23193290
435DSLGHTASPKMQTQPdbPTM 3.0;UniProt;curated18257517; 16381945; 23193290; 22817900
449PVRSVSQSSATTETNdbPTM 3.0;UniProt18257517; 16381945; 23193290
471NADVPVSSPDYLKDLdbPTM 3.0;UniProt;curated18257517; 16381945; 23193290; 22817900
474VPVSSPDYLKDLVNAdbPTM 3.0;UniProt18257517; 16381945; 23193290
Sequence
(Fasta)		MSARKLQVEI EKTFKKVTDG IAIFDEVYEK LSASNSVSQK EKLEGDLKTQ IKKLQRLRDQ 60
IKTWASSNDI KDKKALLENR RLIEAKMEEF KAVEREMKIK AFSKEGLSIA SKLDPKEKEK 120
QDTIQWISNA VEELERQAEL IEAEAESLKA TFKRGKKDLS KLSHLSELES RIERHKWHQD 180
KLELIMRRLE NSQISPEAVN DIQEDIMYYV ECSQSEDFAE DENLYDELNL DEASASYDAE 240
RSGRSSSSSH SPSPSASSSS SSENLLQDKA EAEEKVSADA SVQDIAEKES LDADKELATN 300
DQEDDEEENQ AETQKDGAIS NNENMQSEVQ TTNPSASTSA VTNITKPTLI QNPSTPLSVS 360
NSKVASPETP NATHTAPKVE MRYASAAAAA AAALAKESPS HHYIMQQVRP ETPNSPRLNS 420
TVIQSKWDSL GHTASPKMQT QPVRSVSQSS ATTETNVKPT KEENADVPVS SPDYLKDLVN 480
ALNTSKEQHK GAIDKEKLTE ALNISCVYVP DATDAAKPQY YIPKDPYPVP HYYPQQPLPL 540
FDSSEMTELV DPDTLFYMFY YRPGTYQQYI AGQELKKQSW RFHKKYTTWF QRHEEPKMIT 600
DEFESGSYRY FDFEGDWVQR KKADFRFTYQ YLEDDDDWTR 641
Keyword

KW-0010--Activator
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0678--Repressor
KW-0804--Transcription
KW-0805--Transcription regulation
Interpro

IPR007282--NOT
IPR007207--Not_N
PROSITE
Pfam

PF04153--NOT2_3_5
PF04065--Not3
Gene Ontology

GO:0030015--C:CCR4-NOT core complex
GO:0005737--C:cytoplasm
GO:0005829--C:cytosol
GO:0005634--C:nucleus
GO:0006402--P:mRNA catabolic process
GO:0000289--P:nuclear-transcribed mRNA poly(A) tail shortening
GO:0006357--P:regulation of transcription from RNA polymerase II promoter
GO:0006351--P:transcription, DNA-templated