| Tag |
Content |
| dbPAF ID |
dbPAF-0000513 |
| Uniprot Accession |
O13836; DXO_SCHPO;
|
| Genbank Protein ID |
NP_594904.1;
|
| Genbank Nucleotide ID |
NM_001020334.2;
|
| Protein Name |
Decapping nuclease din1 |
| Protein Synonyms/Alias |
Dhp1-interacting protein 1; |
| Gene Name |
din1 |
| Gene Synonyms/Alias |
SPAC19D5.06c; |
| Organism |
Schizosaccharomyces pombe (strain 972 / ATCC 24843)(Fission yeast) |
| NCBI Taxa ID |
284812 |
Functional Description
(View all) |
Ribonuclease that specifically degrades pre-mRNAs with a defective 5' end cap and is part of a pre-mRNA capping quality control. Has decapping and pyrophosphohydrolase activities. Has decapping activity toward incomplete 5' end cap mRNAs such as unmethylated 5' end-capped RNA to release GpppN and 5' end monophosphate RNA (By similarity). Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5' end triphosphate to release pyrophosphates. Stimulates exoribonuclease activity of rat1, allowing it to degrade RNAs with stable secondary structure more effectively.Functional Description
Ribonuclease that specifically degrades pre-mRNAs with a defective 5' end cap and is part of a pre-mRNA capping quality control. Has decapping and pyrophosphohydrolase activities. Has decapping activity toward incomplete 5' end cap mRNAs such as unmethylated 5' end-capped RNA to release GpppN and 5' end monophosphate RNA (By similarity). Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5' end triphosphate to release pyrophosphates. Stimulates exoribonuclease activity of rat1, allowing it to degrade RNAs with stable secondary structure more effectively. |
Phosphorylation Sites
|
dbPAF PTMs: 2 |
Sequence
(Fasta) | MLREFSFYDV PPAHVPPVSE PLEIACYSLS RDRELLLDDS KLSYYYPPPL FSDLNTGFPN 60
RFHPPKSDPD PISIVKDVLM TKGIQMNSSF LTWRGLITKI MCAPLDPRNH WETYLVMDPT 120
SGIIMMEERT RSETSYANQD RMCYWGYKFE AISTLPEIWD ACSRDQIEQR DNQDVVPDEQ 180
YCSIVKINIG KSKLILAGEV DCIWDKKPCS AKESDVHSDD GTIEEDASNA ENPNLHYVEL 240
KTSKKYPLEN YGMRKKLLKY WAQSFLLGIG RIIIGFRDDN GILIEMKELF THQIPKMLRP 300
YFKPNDWTPN RLLVVLEHAL EWIKQTVKQH PPSTEFTLSY TGGSKLVLRQ II
353Fasta Sequence
>O13836|din1|Schizosaccharomyces pombe (strain 972 / ATCC 24843)(Fission yeast)
MLREFSFYDVPPAHVPPVSEPLEIACYSLSRDRELLLDDSKLSYYYPPPLFSDLNTGFPNRFHPPKSDPDPISIVKDVLMTKGIQMNSSFLTWRGLITKIMCAPLDPRNHWETYLVMDPTSGIIMMEERTRSETSYANQDRMCYWGYKFEAISTLPEIWDACSRDQIEQRDNQDVVPDEQYCSIVKINIGKSKLILAGEVDCIWDKKPCSAKESDVHSDDGTIEEDASNAENPNLHYVELKTSKKYPLENYGMRKKLLKYWAQSFLLGIGRIIIGFRDDNGILIEMKELFTHQIPKMLRPYFKPNDWTPNRLLVVLEHALEWIKQTVKQHPPSTEFTLSYTGGSKLVLRQII
|
| Keyword |
KW-0002--3D-structure
KW-0181--Complete proteome
KW-0378--Hydrolase
KW-0479--Metal-binding
KW-0507--mRNA processing
KW-0540--Nuclease
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0694--RNA-binding
KW-0698--rRNA processing
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0806--Transcription termination
|
| Interpro |
IPR013961--RAI1
|
| PROSITE |
|
| Pfam |
PF08652--RAI1
|
| Gene Ontology |
GO:0005829--C:cytosol
GO:0005634--C:nucleus
GO:0019003--F:GDP binding
GO:0046872--F:metal ion binding
GO:1990174--F:phosphodiesterase decapping endonuclease activity
GO:0003723--F:RNA binding
GO:0034353--F:RNA pyrophosphohydrolase activity
GO:0006402--P:mRNA catabolic process
GO:0006397--P:mRNA processing
GO:0071035--P:nuclear polyadenylation-dependent rRNA catabolic process
GO:0000956--P:nuclear-transcribed mRNA catabolic process
GO:1901919--P:positive regulation of exoribonuclease activity
GO:0006355--P:regulation of transcription, DNA-templated
GO:0090502--P:RNA phosphodiester bond hydrolysis, endonucleolytic
GO:0006364--P:rRNA processing
GO:0030846--P:termination of RNA polymerase II transcription, poly(A)-coupled
|
