dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000494
Uniprot Accession O13775; SYEC_SCHPO;
Genbank Protein ID NP_593483.1;
Genbank Nucleotide ID NM_001018916.2;
Protein Name Probable glutamate--tRNA ligase, cytoplasmic
Protein Synonyms/Alias Glutamyl-tRNA synthetase;GluRS;
Gene Name gus1
Gene Synonyms/Alias ers1;SPAC17A5.15c;
Organism Schizosaccharomyces pombe (strain 972 / ATCC 24843)(Fission yeast)
NCBI Taxa ID 284812
Functional Description
(View all)		 Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).
Phosphorylation Sites
 dbPAF PTMs: 1
PositionPeptidesSourceReferences ( PMIDs )
190NISKKQKSSGPNYEIdbPTM 3.0;UniProt18257517; 16381945; 23193290
Sequence
(Fasta)		MSVSVALKAK PIAYGAVACA NYVNLSGAGS ISVKYEDVAL LDKQNKESNV SIQLNGSDSP 60
VFGSKLALQT FSQVYPKLFI GENDRSLVES WVETASALAG NHNFLELSSL LAQLDDHLIM 120
RSLFVGYSLT SADFSIWGAL KSNNMAAGAV RTGQYFNLAR WYKFMDSQNA VSVTMEEFTK 180
AVNISKKQKS SGPNYEIGLP DAIDGKVVTR FPPEPSGYLH IGHAKAALLN QYFANKYHGK 240
LIVRFDDTNP SKENSEFQDA ILEDVALLGI KPDVVTYTSD YLDTIHQYCV DMIKSGQAYA 300
DDTDVETMRH ERTEGIPSKH RDRPIEESLE ILSEMDKGSD VGLKNCIRAK ISYENPNKAM 360
RDPVIYRCNL LPHHRTGTKY RAYPTYDFAC PIVDSLEGVT HALRTTEYRD RNPLYQWMIK 420
AMNLRKIHVW EFSRMNFVRT LLSKRKLTEI VDHGLVWGWD DPRFPTVRGV RRRGMTIEAL 480
QQYIVSQGPS KNILTLDWTS FWATNKKIID PVAPRHTAVE SGDVVKATIV NGPAAPYAED 540
RPRHKKNPEL GNKKSIFANE ILIEQADAQS FKQDEEVTLM DWGNAYVREI NRDASGKVTS 600
LKLELHLDGD FKKTEKKVTW LADTEDKTPV DLVDFDYLIT KDKLEEGENY KDFLTPQTEF 660
HSPVFADVGI KNLKKGDIIQ VERKGYYIVD VPFDGTQAVL FNIPDGKTVN RYGVKN 717
Keyword

KW-0030--Aminoacyl-tRNA synthetase
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0436--Ligase
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-0648--Protein biosynthesis
KW-1185--Reference proteome
Interpro

IPR001412--aa-tRNA-synth_I_CS
IPR004526--Glu-tRNA-synth_arc/euk
IPR000924--Glu/Gln-tRNA-synth
IPR020061--Glu/Gln-tRNA-synth_Ib_a-bdl
IPR020058--Glu/Gln-tRNA-synth_Ib_cat-dom
IPR020059--Glu/Gln-tRNA-synth_Ib_codon-bd
IPR010987--Glutathione-S-Trfase_C-like
IPR020056--Rbsml_L25/Gln-tRNA_synth_b-brl
IPR011035--Ribosomal_L25/Gln-tRNA_synth
IPR014729--Rossmann-like_a/b/a_fold
PROSITE

PS00178--AA_TRNA_LIGASE_I
Pfam

PF00749--tRNA-synt_1c
PF03950--tRNA-synt_1c_C
Gene Ontology

GO:0005829--C:cytosol
GO:0005634--C:nucleus
GO:0005524--F:ATP binding
GO:0004818--F:glutamate-tRNA ligase activity
GO:0002181--P:cytoplasmic translation
GO:0006424--P:glutamyl-tRNA aminoacylation