dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000452
Uniprot Accession O13535; YH11B_YEAST; D3DLG6;
Genbank Protein ID NP_012086.1;
Genbank Nucleotide ID NM_001181431.2;
Protein Name Transposon Ty1-H Gag-Pol polyprotein
Protein Synonyms/Alias Gag-Pol-p199;TY1A-TY1B;Transposon Ty1 TYA-TYB polyprotein;p190;
Gene Name TY1B-H
Gene Synonyms/Alias YHRCTy1-1 POL;YHR214C-B;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Capsid protein (CA) is the structural component of the virus-like particle (VLP), forming the shell that encapsulates the retrotransposons dimeric RNA genome. The particles are assembled from trimer-clustered units and there are holes in the capsid shells that allow for the diffusion of macromolecules. CA has also nucleocapsid-like chaperone activity, promoting primer tRNA(i)-Met annealing to the multipartite primer-binding site (PBS), dimerization of Ty1 RNA and initiation of reverse transcription (By similarity).
Phosphorylation Sites
 dbPAF PTMs: 66 (View all)
PositionPeptidesSourceReferences ( PMIDs )
13LSNYPHISHGSACASdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated15665377; 16381945; 23193290; 19366988; 26040289; 22817900
16YPHISHGSACASVTSSysPTM 2.0;curated18407956; 19366988; 26040289
20SHGSACASVTSKEVHPhosphoGRID 2.0;SysPTM 2.0;curated18407956; 19366988
22GSACASVTSKEVHTNPhosphoGRID 2.0;SysPTM 2.0;curated18407956; 19366988
28VTSKEVHTNQDPLDVPhosphoGRID 2.0;curated19779198; 22817900
36NQDPLDVSASKIQEYPhosphoGRID 2.0;PHOSIDA;curated18407956; 19779198; 21081558; 26040289; 22817900; 25521595;
38DPLDVSASKIQEYDKPHOSIDA;curated21081558; 26040289; 19795423; 25521595; 24961812; 18407956
43SASKIQEYDKASTKAPhosphoPep 2.019060867
47IQEYDKASTKANSQQPhosphoGRID 2.0;SysPTM 2.0;curated21177495; 18407956; 19779198; 17563356; 17287358; 19366988;
48QEYDKASTKANSQQTPhosphoPep 2.0;curated19060867; 24961812
71PENLHHASPQPASVPPhosphoGRID 2.0;curated18407956
145GTPLSTPSPESGNTFPhosphoGRID 2.0;SysPTM 2.0;curated18407956; 19366988
157NTFTDSSSADSDMTScurated16445868
160TDSSSADSDMTSTKKPhosphoGRID 2.0;SysPTM 2.0;curated18407956; 19366988; 16445868
176VRPPPMLTSPNDFPNPhosphoGRID 2.0;SysPTM 2.017330950; 19366988
177RPPPMLTSPNDFPNWPhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated19779198; 17330950; 19060867; 19366988; 26040406; 22817900;
318ANLQYNGSTPADAFEPhosphoGRID 2.0;SysPTM 2.0;curated18407956; 19366988
356QLIMRGLSGEYKFLRcurated26040289; 22817900
391IYEEQQGSRNSKPNYPhosphoGRID 2.0;SysPTM 2.0;curated18407956; 19366988
403PNYRRNPSDEKNDSRPhosphoGRID 2.0;curated21177495; 22817900
Sequence
(Fasta)		MESQQLSNYP HISHGSACAS VTSKEVHTNQ DPLDVSASKI QEYDKASTKA NSQQTTTPAS 60
SAVPENLHHA SPQPASVPPP QNGPYPQQCM MTQNQANPSG WSFYGHPSMI PYTPYQMSPM 120
YFPPGPQSQF PQYPSSVGTP LSTPSPESGN TFTDSSSADS DMTSTKKYVR PPPMLTSPND 180
FPNWVKTYIK FLQNSNLGGI IPTVNGKPVP PMLTSPNDFP NWVKTYIKFL QNSNLGGIIP 240
TVNGKPVRQI TDDELTFLYN TFQIFAPSQF LPTWVKDILS VDYTDIMKIL SKSIEKMQSD 300
TQEANDIVTL ANLQYNGSTP ADAFETKVTN IIDRLNNNGI HINNKVACQL IMRGLSGEYK 360
FLRYTRHRHL NMTVAELFLD IHAIYEEQQG SRNSKPNYRR NPSDEKNDSR SYTNTTKPKV 420
IARNPQKTNN SKSKTARAHN VSTSNNSPST DNDSISKSTT EPIQLNNKHD LHLGQKLTES 480
TVNHTNHSDD ELPGHLLLDS GASRTLIRSA HHIHSASSNP DINVVDAQKR NIPINAIGDL 540
QFHFQDNTKT SIKVLHTPNI AYDLLSLNEL AAVDITACFT KNVLERSDGT VLAPIVKYGD 600
FYWVSKKYLL PSNISVPTIN NVHTSESTRK YPYPFIHRML AHANAQTIRY SLKNNTITYF 660
NESDVDWSSA IDYQCPDCLI GKSTKHRHIK GSRLKYQNSY EPFQYLHTDI FGPVHNLPKS 720
APSYFISFTD ETTKFRWVYP LHDRREDSIL DVFTTILAFI KNQFQASVLV IQMDRGSEYT 780
NRTLHKFLEK NGITPCYTTT ADSRAHGVAE RLNRTLLDDC RTQLQCSGLP NHLWFSAIEF 840
STIVRNSLAS PKSKKSARQH AGLAGLDIST LLPFGQPVIV NDHNPNSKIH PRGIPGYALH 900
PSRNSYGYII YLPSLKKTVD TTNYVILQGK ESRLDQFNYD ALTFDEDLNR LTASYHSFIA 960
SNEIQQSNDL NIESDHDFQS DIELHPEQLR NVLSKAVSPT DSTPPSTHTE DSKRVSKTNI 1020
RAPREVDPNI SESNILPSKK RSSTPQISDI ESTGSGGMHR LDVPLLAPMS QSNTHESSHA 1080
SKSKDFRHSD SYSDNETNHT NVPISSTGGT NNKTVPQTSE QETEKRIIHR SPSIDTSSSE 1140
SNSLHHVVPI KTSDTCPKEN TEESIIADLP LPDLPPEPPT ELSDSFKELP PINSHQTNSS 1200
LGGIGDSNAY TTINSKKRSL EDNETEIKVS RDTWNTKNMR SLEPPRSKKR IHLIAAVKAV 1260
KSIKPIRTTL RYDEAITYNK DIKEKEKYIQ AYHKEVNQLL MMKTWDTDRY YDRKEIDPKR 1320
VINSMFIFNR KRDGTHKARF VARGDIQHPD TYDPGMQSNT VHHYALMTSL SLALDNNYYI 1380
TQLDISSAYL YADIKEELYI RPPPHLGMND KLIRLKKSLY GLKQSGANWY ETIKSYLIKQ 1440
CGMEEVRGWS CVFKNSQVTI CLFVDDMILF SKDLNANKKI ITTLKKQYDT KIINLGESDN 1500
EIQYDILGLE IKYQRGKYMK LGMENSLTEK IPKLNVPLNP KGRKLSAPGQ PGLYIDQDEL 1560
EIDEDEYKEK VHEMQKLIGL ASYVGYKFRF DLLYYINTLA QHILFPSRQV LDMTYELIQF 1620
MWDTRDKQLI WHKNKPTEPD NKLVAISDAS YGNQPYYKSQ IGNIYLLNGK VIGGKSTKAS 1680
LTCTSTTEAE IHAISESVPL LNNLSHLVQE LNKKPITKGL LTDSKSTISI IISNNEEKFR 1740
NRFFGTKAMR LRDEVSGNHL HVCYIETKKN IADVMTKPLP IKTFKLLTNK WIH 1794
Keyword

KW-0064--Aspartyl protease
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0229--DNA integration
KW-0233--DNA recombination
KW-0238--DNA-binding
KW-0239--DNA-directed DNA polymerase
KW-0255--Endonuclease
KW-0378--Hydrolase
KW-0460--Magnesium
KW-0479--Metal-binding
KW-0511--Multifunctional enzyme
KW-0540--Nuclease
KW-0547--Nucleotide-binding
KW-0548--Nucleotidyltransferase
KW-0539--Nucleus
KW-0645--Protease
KW-1185--Reference proteome
KW-0688--Ribosomal frameshifting
KW-0694--RNA-binding
KW-0695--RNA-directed DNA polymerase
KW-0808--Transferase
KW-0814--Transposable element
KW-0815--Transposition
KW-0917--Virion maturation
KW-1188--Virus exit from host cell
KW-0862--Zinc
KW-0863--Zinc-finger
Interpro

IPR001969--Aspartic_peptidase_AS
IPR001584--Integrase_cat-core
IPR015820--Retrotransposon_Ty1A_N
IPR012337--RNaseH-like_dom
IPR013103--RVT_2
PROSITE

PS00141--ASP_PROTEASE
PS50994--INTEGRASE
Pfam

PF00665--rve
PF07727--RVT_2
PF01021--TYA
Gene Ontology

GO:0005737--C:cytoplasm
GO:0005634--C:nucleus
GO:0000943--C:retrotransposon nucleocapsid
GO:0004190--F:aspartic-type endopeptidase activity
GO:0005524--F:ATP binding
GO:0003677--F:DNA binding
GO:0003887--F:DNA-directed DNA polymerase activity
GO:0046872--F:metal ion binding
GO:0008233--F:peptidase activity
GO:0004540--F:ribonuclease activity
GO:0003723--F:RNA binding
GO:0003964--F:RNA-directed DNA polymerase activity
GO:0004523--F:RNA-DNA hybrid ribonuclease activity
GO:0071897--P:DNA biosynthetic process
GO:0015074--P:DNA integration
GO:0006310--P:DNA recombination
GO:0090501--P:RNA phosphodiester bond hydrolysis
GO:0006278--P:RNA-dependent DNA replication
GO:0032197--P:transposition, RNA-mediated
GO:0019076--P:viral release from host cell