dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000451
Uniprot Accession O13527; YA11B_YEAST; D6VPM1;
Genbank Protein ID NP_009406.1;
Genbank Nucleotide ID NM_001178213.1;
Protein Name Truncated transposon Ty1-A Gag-Pol polyprotein
Protein Synonyms/Alias TY1B;Transposon Ty1 TYB polyprotein;
Gene Name TY1B-A
Gene Synonyms/Alias YARCTy1-1 POL;YAR009C;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Reverse transcriptase/ribonuclease H (RT) is a multifunctional enzyme that catalyzes the conversion of the retro-elements RNA genome into dsDNA within the VLP. The enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes during plus-strand synthesis and hydrolyzes RNA primers. The conversion leads to a linear dsDNA copy of the retrotransposon that includes long terminal repeats (LTRs) at both ends (By similarity).
Phosphorylation Sites
 dbPAF PTMs: 50 (View all)
PositionPeptidesSourceReferences ( PMIDs )
22QISPYPPSIMSIQVKPhosphoGRID 2.017563356
377SNDLNIESDHDFQSDdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19779198; 19366988; 22817900
383ESDHDFQSDIELHPEdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 22817900
397EQPRNVLSKAVSPTDdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17330950; 16381945; 23193290; 21177495; 19684113; 19779198;
401NVLSKAVSPTDSTPPdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated17330950; 17563356; 18407956; 16381945; 23193290; 21177495;
403LSKAVSPTDSTPPSTdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated17563356; 16381945; 23193290; 17330950; 21177495; 19779198;
405KAVSPTDSTPPSTHTdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17563356; 16381945; 23193290; 17330950; 21177495; 19779198;
406AVSPTDSTPPSTHTEdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17563356; 16381945; 23193290; 21177495; 17330950; 19060867;
409PTDSTPPSTHTEDSKcurated22817900; 20377248
412STPPSTHTEDSKRVSPhosphoPep 2.0;curated19060867; 22817900; 20377248
415PSTHTEDSKRVSKTNcurated22817900; 20377248
421DSKRVSKTNIRAPREPhosphoPep 2.019060867
434REVDPNISESNILPSdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 21177495; 19779198;
436VDPNISESNILPSKKPhosphoPep 2.0;curated19060867; 22817900; 24961812; 19547744
445ILPSKKRSSTPQISNdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17330950; 16381945; 23193290; 21177495; 19779198; 19060867;
446LPSKKRSSTPQISNIdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated17330950; 17563356; 18407956; 16381945; 23193290; 21177495;
447PSKKRSSTPQISNIEdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated17330950; 17563356; 18407956; 16381945; 23193290; 21177495;
451RSSTPQISNIESTGSPhosphoGRID 2.0;SysPTM 2.0;curated19779198; 17287358; 19366988; 22817900
455PQISNIESTGSGGMHPhosphoGRID 2.0;SysPTM 2.0;curated17287358; 19366988; 22817900
458SNIESTGSGGMHKLNcurated20377248
Sequence
(Fasta)		METFTGYLKS TCFHQISPYP PSIMSIQVKV HANILILSFI ECLRMPMHRQ IRYSLKNNTI 60
TYFNESDVDW SSAIDYQCPD CLIGKSTKHR HIKGSRLKYQ NSYEPFQYLH TDIFGPVHNL 120
PKSAPSYFIS FTDETTKLRW VYPLHDRRED SILDVFTTIL AFIKNQFQAS VLVIQMDRGS 180
EYTNRTLHKF LEKNGITPCY TTTADSRAHG VAERLNRTLL DDCRTQLQCS GLPNHLWFSA 240
IEFSTIVRNS LASPKSKKSA RQHAGLAGLD ISTLLPFGQP VIVNDHNPNS KIHPRGIPGY 300
ALHPSRNSYG YIIYLPSLKK TVDTTNYVIL QGKESRLDQF NYDALTFDED LNRLTASYHS 360
FIASNEIQES NDLNIESDHD FQSDIELHPE QPRNVLSKAV SPTDSTPPST HTEDSKRVSK 420
TNIRAPREVD PNISESNILP SKKRSSTPQI SNIESTGSGG MHKLNVPLLA PMSQSNTHES 480
SHASKSKDFR HSDSYSENET NHTNVPISST GGTNNKTVPQ ISDQETEKRI IHRSPSIDAS 540
PPENNSSHNI VPIKTPTTVS EQNTEESIIA DLPLPDLPPE SPTEFPDPFK ELPPINSHQT 600
NSSLGGIGDS NAYTTINSKK RSLEDNETEI KVSRDTWNTK NMRSLEPPRS KKRIHLIAAV 660
KAVKSIKPIR TTLRYDEAIT YNKDIKEKEK YIEAYHKEVN QLLKMNTWDT DKYYDRKEID 720
PKRVINSMFI FNKKRDGTHK ARFVARGDIQ HPDTYDTGMQ SNTVHHYALM TSLSLALDNN 780
YYITQLDISS AYLYADIKEE LYIRPPPHLG MNDKLIRLKK SLYGLKQSGA NWYETIKSYL 840
IKQCGMEEVR GWSCVFKNSQ VTICLFVDDM ILFSKDLNAN KKIITTLKKQ YDTKIINLGE 900
SDNEIQYDIL GLEIKYQRGK YMKLGMEKSL TEKLPKLNVP LNPKGKKLRA PGQPGLYIDQ 960
DELEIDEDEY KEKVHEMQKL IGLASYVGYK FRFDLLYYIN TLAQHILFPS RQVLDMTYEL 1020
IQFMWDTRDK QLIWHKNKPT EPDNKLVAIS DASYGNQPYY KSQIGNIYLL NGKVIGGKST 1080
KASLTCTSTT EAEIHAISES VPLLNNLSYL IQELNKKPII KGLLTDSRST ISIIKSTNEE 1140
KFRNRFFGTK AMRLRDEVSG NNLYVYYIET KKNIADVMTK PLPIKTFKLL TNKWIH 1197
Keyword

KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0229--DNA integration
KW-0233--DNA recombination
KW-0238--DNA-binding
KW-0239--DNA-directed DNA polymerase
KW-0255--Endonuclease
KW-0378--Hydrolase
KW-0460--Magnesium
KW-0479--Metal-binding
KW-0511--Multifunctional enzyme
KW-0540--Nuclease
KW-0548--Nucleotidyltransferase
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0694--RNA-binding
KW-0695--RNA-directed DNA polymerase
KW-0808--Transferase
KW-0814--Transposable element
KW-0815--Transposition
Interpro

IPR001584--Integrase_cat-core
IPR012337--RNaseH-like_dom
IPR013103--RVT_2
PROSITE

PS50994--INTEGRASE
Pfam

PF00665--rve
PF07727--RVT_2
Gene Ontology

GO:0005737--C:cytoplasm
GO:0005634--C:nucleus
GO:0000943--C:retrotransposon nucleocapsid
GO:0003677--F:DNA binding
GO:0003887--F:DNA-directed DNA polymerase activity
GO:0046872--F:metal ion binding
GO:0008233--F:peptidase activity
GO:0004540--F:ribonuclease activity
GO:0003723--F:RNA binding
GO:0003964--F:RNA-directed DNA polymerase activity
GO:0004523--F:RNA-DNA hybrid ribonuclease activity
GO:0071897--P:DNA biosynthetic process
GO:0015074--P:DNA integration
GO:0006310--P:DNA recombination
GO:0006508--P:proteolysis
GO:0090501--P:RNA phosphodiester bond hydrolysis
GO:0006278--P:RNA-dependent DNA replication
GO:0032197--P:transposition, RNA-mediated