dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000447
Uniprot Accession O13329; FOB1_YEAST; D6VS96; Q04587;
Genbank Protein ID NP_010395.1;
Genbank Nucleotide ID NM_001180418.1;
Protein Name DNA replication fork-blocking protein FOB1
Protein Synonyms/Alias
Gene Name FOB1
Gene Synonyms/Alias HRM1;YDR110W;YD9727.06;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Required for replication fork blocking activity at the replication fork barrier (RFB) site in rDNA and for recombination hot-spot (HOT1) activity, regulating the recombination rate and the number of rDNA copies. Binds directly to two separated sequences in the RFB.
Phosphorylation Sites
 dbPAF PTMs: 14
PositionPeptidesSourceReferences ( PMIDs )
16VLFDDDDSVPSESVTcurated24961812; 25315811
32KSQRRKATSPGESREcurated19823750; 25315811
33SQRRKATSPGESREScurated25315811
37KATSPGESRESSKDRcurated25315811
456FDLTEKNYIDEYDDLcurated19823750
460EKNYIDEYDDLAHDScurated25704821
467YDDLAHDSSEGEFEPcurated26040289
468DDLAHDSSEGEFEPNcurated26040289
504VAAPMQGTEEPEKGDPhosphoGRID 2.0;curated19823750; 19795423; 22817900
515EKGDQKESDGASQVDcurated20377248
519QKESDGASQVDQSVEdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 20377248
524GASQVDQSVEITRPEcurated20377248
543QTLESPSTKRQKLDQcurated20377248
563QTRDFGTSMEL----curated20377248; 25315811
Sequence
(Fasta)		MTKPRYNDVL FDDDDSVPSE SVTRKSQRRK ATSPGESRES SKDRLLILPS MGESYTEYVD 60
SYLNLELLER GERETPIFLE SLTRQLTQKI YELIKTKSLT ADTLQQISDK YDGVVAENKL 120
LFLQRQYYVD DEGNVRDGRN NDKIYCEPKH VYDMVMATHL MNKHLRGKTL HSFLFSHFAN 180
ISHAIIDWVQ QFCSKCNKKG KIKPLKEYKR PDMYDKLLPM ERIHIEVFEP FNGEAIEGKY 240
SYVLLCRDYR SSFMWLLPLK STKFKHLIPV VSSLFLTFAR VPIFVTSSTL DKDDLYDICE 300
EIASKYGLRI GLGLKSSARF HTGGILCIQY ALNSYKKECL ADWGKCLRYG PYRFNRRRNK 360
RTKRKPVQVL LSEVPGHNAK FETKRERVIE NTYSRNMFKM AGGKGLIYLE DVNTFALANE 420
ADNSCNNNGI LHNNNIGNDN FEEEVQKQFD LTEKNYIDEY DDLAHDSSEG EFEPNTLTPE 480
EKPPHNVDED RIESTGVAAP MQGTEEPEKG DQKESDGASQ VDQSVEITRP ETSYYQTLES 540
PSTKRQKLDQ QGNGDQTRDF GTSMEL 567
Keyword

KW-0181--Complete proteome
KW-0233--DNA recombination
KW-0235--DNA replication
KW-0238--DNA-binding
KW-0479--Metal-binding
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0862--Zinc
KW-0863--Zinc-finger
Interpro
PROSITE
Pfam
Gene Ontology

GO:0005730--C:nucleolus
GO:0033553--C:rDNA heterochromatin
GO:0046872--F:metal ion binding
GO:0043110--F:rDNA spacer replication fork barrier binding
GO:0000183--P:chromatin silencing at rDNA
GO:0007059--P:chromosome segregation
GO:0006310--P:DNA recombination
GO:0006260--P:DNA replication
GO:0007580--P:extrachromosomal circular DNA accumulation involved in cell aging
GO:0043007--P:maintenance of rDNA
GO:0008156--P:negative regulation of DNA replication
GO:0045911--P:positive regulation of DNA recombination
GO:0034503--P:protein localization to nucleolar rDNA repeats
GO:0070550--P:rDNA condensation
GO:0090342--P:regulation of cell aging
GO:0001302--P:replicative cell aging