dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000422
Uniprot Accession O09053; WRN_MOUSE; O09050; Q80YP9; Q9JKD4; Q9Z241; Q9Z242;
Genbank Protein ID NP_001116294.1; NP_035851.3; XP_006509154.1;
Genbank Nucleotide ID NM_001122822.1; NM_011721.4; XM_006509091.2;
Protein Name Werner syndrome ATP-dependent helicase homolog
Protein Synonyms/Alias Exonuclease WRN;
Gene Name Wrn
Gene Synonyms/Alias
Organism Mus musculus(Mouse)
NCBI Taxa ID 10090
Functional Description
(View all)		 Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation (By similarity).
Phosphorylation Sites
 dbPAF PTMs: 14
PositionPeptidesSourceReferences ( PMIDs )
288SISENLCSLRKVICGPhosphoSitePlus22135298
368VKQEKGESENEIEDNdbPTM 3.0;PhosphoSitePlus;curated19854140; 16381945; 23193290; 22135298; 22817900; 25338131
433DSSYIIESDEDLEMEcurated25338131
1044PRKVLLPSSNPVSPEdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;curated21183079; 21659605; 16381945; 23193290; 19060867; 22135298;
1045RKVLLPSSNPVSPETdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;curated21183079; 21659605; 16381945; 23193290; 19060867; 22135298;
1049LPSSNPVSPETTQHSdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;curated21183079; 21659605; 16381945; 23193290; 19060867; 22135298;
1052SNPVSPETTQHSSNQPhosphoSitePlus22135298
1053NPVSPETTQHSSNQNPhosphoSitePlus22135298
1057PETTQHSSNQNPAGLPhosphoSitePlus22135298
1076SNLERTHSYKVPEKVcurated25338131
1098KKSAVMPSPGTSSSPcurated25338131
1102VMPSPGTSSSPLEPAcurated25338131
1342SRKRRFPSSAESCESPhosphoSitePlus22135298
1343RKRRFPSSAESCESCPhosphoSitePlus22135298
Sequence
(Fasta)		METTSLQRKF PEWMSMQSQR CATEEKACVQ KSVLEDNLPF LEFPGSIVYS YEASDCSFLS 60
EDISMRLSDG DVVGFDMEWP PIYKPGKRSR VAVIQLCVSE SKCYLFHISS MSVFPQGLKM 120
LLENKSIKKA GVGIEGDQWK LLRDFDVKLE SFVELTDVAN EKLKCAETWS LNGLVKHVLG 180
KQLLKDKSIR CSNWSNFPLT EDQKLYAATD AYAGLIIYQK LGNLGDTAQV FALNKAEENL 240
PLEMKKQLNS ISEEMRDLAN RFPVTCRNLE TLQRVPVILK SISENLCSLR KVICGPTNTE 300
TRLKPGSSFN LLSSEDSAAA GEKEKQIGKH STFAKIKEEP WDPELDSLVK QEEVDVFRNQ 360
VKQEKGESEN EIEDNLLRED MERTCVIPSI SENELQDLEQ QAKEEKYNDV SHQLSEHLSP 420
NDDENDSSYI IESDEDLEME MLKSLENLNS DVVEPTHSTW LEMGTNGRLP PEEEDGHGNE 480
AIKEEQEEED HLLPEPNAKQ INCLKTYFGH SSFKPVQWKV IHSVLEERRD NVVVMATGYG 540
KSLCFQYPPV YTGKIGIVIS PLISLMEDQV LQLELSNVPA CLLGSAQSKN ILGDVKLGKY 600
RVIYITPEFC SGNLDLLQQL DSSIGITLIA VDEAHCISEW GHDFRSSFRM LGSLKTALPL 660
VPVIALSATA SSSIREDIIS CLNLKDPQIT CTGFDRPNLY LEVGRKTGNI LQDLKPFLVR 720
KASSAWEFEG PTIIYCPSRK MTEQVTAELG KLNLACRTYH AGMKISERKD VHHRFLRDEI 780
QCVVATVAFG MGINKADIRK VIHYGAPKEM ESYYQEIGRA GRDGLQSSCH LLWAPADFNT 840
SRNLLIEIHD EKFRLYKLKM MVKMEKYLHS SQCRRRIILS HFEDKCLQKA SLDIMGTEKC 900
CDNCRPRLNH CLTANNSEDA SQDFGPQAFQ LLSAVDILQE KFGIGIPILF LRGSNSQRLP 960
DKYRGHRLFG AGKEQAESWW KTLSHHLIAE GFLVEVPKEN KYIKTCSLTK KGRKWLGEAS 1020
SQSPPSLLLQ ANEEMFPRKV LLPSSNPVSP ETTQHSSNQN PAGLTTKQSN LERTHSYKVP 1080
EKVSSGTNIP KKSAVMPSPG TSSSPLEPAI SAQELDARTG LYARLVEARQ KHANKMDVPP 1140
AILATNKVLL DMAKMRPTTV ENMKQIDGVS EGKAALLAPL LEVIKHFCQV TSVQTDLLSS 1200
AKPHKEQEKS QEMEKKDCSL PQSVAVTYTL FQEKKMPLHS IAENRLLPLT AAGMHLAQAV 1260
KAGYPLDMER AGLTPETWKI IMDVIRNPPI NSDMYKVKLI RMLVPENLDT YLIHMAIEIL 1320
QSGSDSRTQP PCDSSRKRRF PSSAESCESC KESKEAVTET KASSSESKRK LPEWFAKGNV 1380
PSADTGSSSS MAKTKKKGLF S 1402
Keyword

KW-0002--3D-structure
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0227--DNA damage
KW-0234--DNA repair
KW-0238--DNA-binding
KW-0269--Exonuclease
KW-0347--Helicase
KW-0378--Hydrolase
KW-0460--Magnesium
KW-0479--Metal-binding
KW-0511--Multifunctional enzyme
KW-0540--Nuclease
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0862--Zinc
Interpro

IPR002562--3'-5'_exonuclease_dom
IPR011545--DEAD/DEAH_box_helicase_dom
IPR004589--DNA_helicase_ATP-dep_RecQ
IPR014001--Helicase_ATP-bd
IPR001650--Helicase_C
IPR029491--Helicase_HTH
IPR010997--HRDC-like
IPR002121--HRDC_dom
IPR027417--P-loop_NTPase
IPR032284--RecQ_Zn-bd
IPR012337--RNaseH-like_dom
IPR018982--RQC_domain
IPR011991--WHTH_DNA-bd_dom
PROSITE

PS51192--HELICASE_ATP_BIND_1
PS51194--HELICASE_CTER
PS50967--HRDC
Pfam

PF00270--DEAD
PF01612--DNA_pol_A_exo1
PF00271--Helicase_C
PF00570--HRDC
PF14493--HTH_40
PF16124--RecQ_Zn_bind
PF09382--RQC
Gene Ontology

GO:0005813--C:centrosome
GO:0032389--C:MutLalpha complex
GO:0005730--C:nucleolus
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0043138--F:3'-5' DNA helicase activity
GO:0008408--F:3'-5' exonuclease activity
GO:0005524--F:ATP binding
GO:0043140--F:ATP-dependent 3'-5' DNA helicase activity
GO:0004003--F:ATP-dependent DNA helicase activity
GO:0016887--F:ATPase activity
GO:0000405--F:bubble DNA binding
GO:0003677--F:DNA binding
GO:0003678--F:DNA helicase activity
GO:0004527--F:exonuclease activity
GO:0009378--F:four-way junction helicase activity
GO:0051880--F:G-quadruplex DNA binding
GO:0004386--F:helicase activity
GO:0000287--F:magnesium ion binding
GO:0030145--F:manganese ion binding
GO:0032403--F:protein complex binding
GO:0042803--F:protein homodimerization activity
GO:0000403--F:Y-form DNA binding
GO:0006284--P:base-excision repair
GO:0007569--P:cell aging
GO:0006974--P:cellular response to DNA damage stimulus
GO:0071480--P:cellular response to gamma radiation
GO:0009267--P:cellular response to starvation
GO:0032508--P:DNA duplex unwinding
GO:0006259--P:DNA metabolic process
GO:0006310--P:DNA recombination
GO:0006260--P:DNA replication
GO:0000731--P:DNA synthesis involved in DNA repair
GO:0006302--P:double-strand break repair
GO:0010259--P:multicellular organismal aging
GO:0090305--P:nucleic acid phosphodiester bond hydrolysis
GO:0032066--P:nucleolus to nucleoplasm transport
GO:0051345--P:positive regulation of hydrolase activity
GO:0042981--P:regulation of apoptotic process
GO:0040009--P:regulation of growth rate
GO:0031297--P:replication fork processing
GO:0001302--P:replicative cell aging
GO:0006979--P:response to oxidative stress
GO:0010225--P:response to UV-C
GO:0000723--P:telomere maintenance