dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000393
Uniprot Accession O08908; P85B_MOUSE; Q5U3K7;
Genbank Protein ID NP_032867.2;
Genbank Nucleotide ID NM_008841.2;
Protein Name Phosphatidylinositol 3-kinase regulatory subunit beta
Protein Synonyms/Alias
Gene Name Pik3r2
Gene Synonyms/Alias
Organism Mus musculus(Mouse)
NCBI Taxa ID 10090
Functional Description
(View all)		 Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (By similarity). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348926).
Phosphorylation Sites
 dbPAF PTMs: 14
PositionPeptidesSourceReferences ( PMIDs )
195PEAAAEAYRALREVAPhosphoSitePlus22135298
267SGGEGDGSEPVPDFPcurated25338131
360SKIQGEYTLTLRKGGPhosphoSitePlus22135298
417KLDTRLLYPVSKYQQPhosphoSitePlus22135298
443VGAQLKVYHQQYQDKcurated25195567; 22817900
447LKVYHQQYQDKSREYPhosphoSitePlus22135298
458SREYDQLYEEYTRTSdbPTM 3.0;Phospho.ELM 9.0;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;UniProt;curated17947660; 18034455; 16381945; 23193290; 15592455; 18515860;
461YDQLYEEYTRTSQELPhosphoSitePlus;curated22135298; 22817900
599KNETEDQYSLMEDEDdbPTM 3.0;PhosphoSitePlus;curated18515860; 16381945; 23193290; 22135298; 22817900
600NETEDQYSLMEDEDAdbPTM 3.0;PhosphoSitePlus;curated18515860; 16381945; 23193290; 22135298; 22817900
637LSGKRDGTFLIRESSPhosphoSitePlus22135298
649ESSQRGCYACSVVVDPhosphoSitePlus22135298
652QRGCYACSVVVDGDTPhosphoSitePlus22135298
665DTKHCVIYRTATGFGPhosphoSitePlus22135298
Sequence
(Fasta)		MAGAEGFQYR AVYPFRRERP EDLELLPGDL LVVSRVALQA LGVADGGERC PHNVGWMPGF 60
NERTRQRGDF PGTYVEFLGP VALARPGPRP RGPRPLPARP LDGSSESGHI LPDLAEQFSP 120
PDPAPPILVK LVEAIEQAEL DSECYSKPEL PATRTDWSLS DLEQWDRTAL YDAVKGFLLA 180
LPAAVVTPEA AAEAYRALRE VAGPVGLVLE PPTLPLHQAL TLRFLLQHLG RVARRAPSPD 240
TAVHALASAF GPLLLRIPPS GGEGDGSEPV PDFPVLLLER LVQEHVEEQD AAPPALPPKP 300
SKAKPAPTAL ANGGSPPSLQ DAEWYWGDIS REEVNERLRD TPDGTFLVRD ASSKIQGEYT 360
LTLRKGGNNK LIKVFHRDGH YGFSEPLTFC SVVELISHYR HESLAQYNAK LDTRLLYPVS 420
KYQQDQVVKE DSIEAVGAQL KVYHQQYQDK SREYDQLYEE YTRTSQELQM KRTAIEAFNE 480
TIKIFEEQGQ TQEKCSKEYL ERFRREGNEK EMQRILLNSE RLKSRIAEIH ESRTKLEQDL 540
RAQASDNREI DKRMNSLKPD LMQLRKIRDQ YLVWLTQKGA RQRKINEWLG IKNETEDQYS 600
LMEDEDALPH HEERTWYVGK INRTQAEEML SGKRDGTFLI RESSQRGCYA CSVVVDGDTK 660
HCVIYRTATG FGFAEPYNLY GSLKELVLHY QHASLVQHND ALTVTLAHPV RAPGPGPPSA 720
AR 723
Keyword

KW-0002--3D-structure
KW-0181--Complete proteome
KW-0597--Phosphoprotein
KW-0653--Protein transport
KW-1185--Reference proteome
KW-0677--Repeat
KW-0727--SH2 domain
KW-0728--SH3 domain
KW-0346--Stress response
KW-0813--Transport
KW-0832--Ubl conjugation
Interpro

IPR032498--PI3K_P85_iSH2
IPR008936--Rho_GTPase_activation_prot
IPR000198--RhoGAP_dom
IPR000980--SH2
IPR001452--SH3_domain
PROSITE

PS50238--RHOGAP
PS50001--SH2
PS50002--SH3
Pfam

PF16454--PI3K_P85_iSH2
PF00620--RhoGAP
PF00017--SH2
Gene Ontology

GO:0005925--C:focal adhesion
GO:0005634--C:nucleus
GO:0005942--C:phosphatidylinositol 3-kinase complex
GO:0046935--F:1-phosphatidylinositol-3-kinase regulator activity
GO:0036312--F:phosphatidylinositol 3-kinase regulatory subunit binding
GO:0046982--F:protein heterodimerization activity
GO:0019903--F:protein phosphatase binding
GO:0030971--F:receptor tyrosine kinase binding
GO:0008134--F:transcription factor binding
GO:0001678--P:cellular glucose homeostasis
GO:0032869--P:cellular response to insulin stimulus
GO:0008286--P:insulin receptor signaling pathway
GO:0014065--P:phosphatidylinositol 3-kinase signaling
GO:0046854--P:phosphatidylinositol phosphorylation
GO:0036092--P:phosphatidylinositol-3-phosphate biosynthetic process
GO:0045785--P:positive regulation of cell adhesion
GO:0042993--P:positive regulation of transcription factor import into nucleus
GO:0045944--P:positive regulation of transcription from RNA polymerase II promoter
GO:0015031--P:protein transport
GO:0030833--P:regulation of actin filament polymerization
GO:0010506--P:regulation of autophagy
GO:0090003--P:regulation of establishment of protein localization to plasma membrane
GO:0043551--P:regulation of phosphatidylinositol 3-kinase activity
GO:0042325--P:regulation of phosphorylation
GO:0051492--P:regulation of stress fiber assembly
GO:0034976--P:response to endoplasmic reticulum stress