dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000386
Uniprot Accession O08874; PKN2_RAT;
Genbank Protein ID
Genbank Nucleotide ID
Protein Name Serine/threonine-protein kinase N2
Protein Synonyms/Alias Cardiolipin-activated protein kinase Pak2;PKN gamma;Protease-activated kinase 2;PAK-2;Protein kinase C-like 2;Protein-kinase C-related kinase 2;p140 kinase;
Gene Name Pkn2
Gene Synonyms/Alias Pak2;Prk2;Prkcl2;
Organism Rattus norvegicus(Rat)
NCBI Taxa ID 10116
Functional Description
(View all)		 PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF) (By similarity).
Phosphorylation Sites
 dbPAF PTMs: 11
PositionPeptidesSourceReferences ( PMIDs )
361SVALPGWSPSENRSScurated23984901
583PPAPPRASSLGEIDDPhosphoSitePlus;curated22135298; 23712012; 21630457; 23984901
584PAPPRASSLGEIDDSPhosphoSitePlus;UniProt;curated22135298; 22673903; 25532521; 23712012; 21630457
621RNNMRPKSKSEYELNcurated21630457; 22276854
623NMRPKSKSEYELNIPcurated21630457
625RPKSKSEYELNIPDScurated21630457; 22276854
815GMGYGDRTSTFCGTPPhosphoSitePlus22135298
817GYGDRTSTFCGTPEFPhosphoSitePlus;curated22135298; 23712012; 21630457
821RTSTFCGTPEFLAPEcurated23712012; 21630457; 23984901
952SNFDDEFTSEAPILTdbPTM 3.0;Phospho.ELM 9.010753910; 16381945; 23193290
959TSEAPILTPPREPRIdbPTM 3.0;PhosphoSitePlus;UniProt;curated17683130; 16381945; 23193290; 22135298; 22673903; 23984901
Sequence
(Fasta)		MASNPDRGEI LLTELQVDSR PLPFSENVSA VQKLDFSDTI VQQKLDDVKD RIKREIRKEL 60
KIKEGAENLR KVTTDKKNLA YVDNILKKSN KKLEELHHKL QELNAHIVVS DPEDYTDCPR 120
TPDTPNSDSR SSTSNNRRLM ALQKQLDIEL KVKQGAENMI QMYSNGPSKD RKLHGTAQQL 180
LQDNKTKIEV IRMHILQAVL TNELAFDNAK PVISPLELRN GRIIEHHFRI EFAVAEGAKN 240
VMKLLGSGKV TDRKALSEAQ ARFNESSQKL DLLKYSLEQR LNELPKNHPK SSVVIEELSL 300
VASPTLSPRQ SMLSTQNQYS TLSKPAALTG TLEVRLWGAK ISWENVPGRS KATSVALPGW 360
SPSENRSSFM SRTSKSKSGS SRNLLKTDDL SNDVCAVLKL DNTVVGQTIW KPISNQSWDQ 420
KFTLELDRSR ELEISVYWRD WRSLCAVKFL RLEDFLDNQR HGMALYLEPQ GTLFAEVTFF 480
NPVIERRPKL QRQKKIFSKQ QGKTFLRAPQ MNINIATWGR LVRRAIPTVN HSGTFSPQTP 540
VPATVPVVDA RTPELAPPAS DSTVTKLDFD LEPEAPPAPP RASSLGEIDD SSELRVLDIP 600
GQGSETVFDI ENDRNNMRPK SKSEYELNIP DSSRSCWSVG ELEDKRSQQR FQFNLQDFRC 660
CAVLGRGHFG KVLLAEYKHT NEMFAIKALK KGDIVARDEV DSLMCEKRIF ETVNSVRHPF 720
LVNLFACFQT KEHVCFVMEY AAGGDLMMHI HTDVFSEPRA VFYAACVVLG LQYLHEHKIV 780
YRDLKLDNLL LDTEASVKIA DFGLCKEGMG YGDRTSTFCG TPEFLAPEVL TETSYTRAVD 840
WWGLGVLIYE MLVGESPFPG DDEEEVFDSI VNDEVRYPRF LSTEAISIMR RLLRRNPERR 900
LGAGEKDAED VKKHPFFRLT DWSALLDKKV KPPFVPTIRG REDVSNFDDE FTSEAPILTP 960
PREPRILLEE EQEMFRDFDY VADWC 986
Keyword

KW-0007--Acetylation
KW-0053--Apoptosis
KW-0067--ATP-binding
KW-0130--Cell adhesion
KW-0131--Cell cycle
KW-0132--Cell division
KW-0965--Cell junction
KW-0966--Cell projection
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0206--Cytoskeleton
KW-0903--Direct protein sequencing
KW-0418--Kinase
KW-0472--Membrane
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0723--Serine/threonine-protein kinase
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0808--Transferase
Interpro

IPR000961--AGC-kinase_C
IPR000008--C2_dom
IPR011072--HR1_rho-bd
IPR011009--Kinase-like_dom
IPR017892--Pkinase_C
IPR000719--Prot_kinase_dom
IPR017441--Protein_kinase_ATP_BS
IPR002290--Ser/Thr_dual-sp_kinase
IPR008271--Ser/Thr_kinase_AS
PROSITE

PS51285--AGC_KINASE_CTER
PS00107--PROTEIN_KINASE_ATP
PS50011--PROTEIN_KINASE_DOM
PS00108--PROTEIN_KINASE_ST
Pfam

PF02185--HR1
PF00069--Pkinase
PF00433--Pkinase_C
Gene Ontology

GO:0043296--C:apical junction complex
GO:0032154--C:cleavage furrow
GO:0005737--C:cytoplasm
GO:0005856--C:cytoskeleton
GO:0030027--C:lamellipodium
GO:0016020--C:membrane
GO:0030496--C:midbody
GO:0005634--C:nucleus
GO:0005524--F:ATP binding
GO:0042826--F:histone deacetylase binding
GO:0004697--F:protein kinase C activity
GO:0004674--F:protein serine/threonine kinase activity
GO:0043297--P:apical junction assembly
GO:0006915--P:apoptotic process
GO:0007155--P:cell adhesion
GO:0007049--P:cell cycle
GO:0051301--P:cell division
GO:0010631--P:epithelial cell migration
GO:0032467--P:positive regulation of cytokinesis
GO:0045931--P:positive regulation of mitotic cell cycle
GO:0006468--P:protein phosphorylation
GO:2000145--P:regulation of cell motility
GO:0006355--P:regulation of transcription, DNA-templated
GO:0007165--P:signal transduction
GO:0006351--P:transcription, DNA-templated