dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000385
Uniprot Accession O08873; MADD_RAT;
Genbank Protein ID NP_446037.1;
Genbank Nucleotide ID NM_053585.1;
Protein Name MAP kinase-activating death domain protein
Protein Synonyms/Alias Rab3 GDP/GTP exchange factor;
Gene Name Madd
Gene Synonyms/Alias
Organism Rattus norvegicus(Rat)
NCBI Taxa ID 10116
Functional Description
(View all)		 Plays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis).
Phosphorylation Sites
 dbPAF PTMs: 13
PositionPeptidesSourceReferences ( PMIDs )
155STPDVNQSPRGKRRAcurated22276854
688GEEPGPDSENSQENLPhosphoSitePlus;UniProt22135298; 22673903
691PGPDSENSQENLPLRPhosphoSitePlus;UniProt22135298; 22673903
778YFEPQYGSPAEEDDDPhosphoSitePlus;UniProt;curated22135298; 22673903; 23589303
812QKLLRPNSLKLASDSdbPTM 3.0;PhosphoSitePlus;SysPTM 2.0;UniProt;curated16641100; 16381945; 23193290; 22135298; 19366988; 22673903;
861RNHSTSFSLSNLTLPcurated23984901
909TVKREPPSPQGRSSNPhosphoSitePlus;UniProt22135298; 22673903
1038EPDKRKRSPTENVNTPhosphoSitePlus;UniProt22135298; 22673903
1040DKRKRSPTENVNTPVPhosphoSitePlus;UniProt22135298; 22673903
1045SPTENVNTPVGKDPGPhosphoSitePlus;UniProt22135298; 22673903
1194HLASSRATLSDSEIEcurated23984901
1196ASSRATLSDSEIETNPhosphoSitePlus;UniProt;curated22135298; 22673903; 23589303
1198SRATLSDSEIETNSAcurated25532521
Sequence
(Fasta)		MVQKKFCPRL LDYLVIVGAR HPSSDSVAQT PELLRRYPLE DHPEFPLPPD VVFFCQPEGC 60
LSVRQRRMSL RDDTSFVFTL TDKDTGVTRY GICVNFYRSF QKRMPKEKAE GGAGPRGKEG 120
AHAPCASEEA ATESSESGST LQPPSADSTP DVNQSPRGKR RAKAGNRSRN STLTSLCVLS 180
HYPFFSTFRE CLYTLKRLVD CCSERLLGKK PGIPRGVQRD TMWRIFTGSL LVEEKSSALL 240
HDLREIEAWI YRLLRSPVPV SGQKRVDIEV LPQEVQQALT FALPDPSRFT LVDFPLHLPL 300
ELLGVDACLQ VLTCILLEHK VVLQSRDYNA LSMSVMAFVA MIYPLEYMFP VIPLLPTCMA 360
SAEQLLLAPT PYIIGVPASF FLYKLDFKMP DDVWLVDLDS NRVIAPTNAE VLPILPEPES 420
LELKKHLKQA LASMSLNTQP ILNLEKFHEG QETPLLLGRF SNDLQSTPST EFNPLIYGND 480
VDSVDVATRV AMVRFFNSAN VLQGFQMHTR TLRLFPRPVV AFQAGSFLAS RPRQTPFAEK 540
LARTQAVEYF GEWILNPSNY AFQRIHNNTF DPALIGDKPK WYAHQLQPIH YRVYDSNSQL 600
AEALSVPPER DSESDPTDDS GSDSMDYDDS SSSYSSLGDF VSEMMKCDIN GDTPNVDPLT 660
HAALGDASEV EIDELQPQKE GEEPGPDSEN SQENLPLRSS SSTTASSSPS TIVHGAHSEP 720
ADSTEVGDKA ATGISKPLPP VPPSICKSTV DRRQTETGEG SVCQRTYDHP YFEPQYGSPA 780
EEDDDEQGES YTPRFSQHAS GSRAQKLLRP NSLKLASDSD AESDSRASSP NSTVSNNSTE 840
GFGGIMSFAS SLYRNHSTSF SLSNLTLPTK GAREKTTPFP SLKGNRRALV DQKSSVIKHS 900
PTVKREPPSP QGRSSNSSEN QQFLKEVVHS VLDGQGVGWL NMKKVRRLLE SEQLRVFVLS 960
KLSRAVQSED DARQDVIQDV EISRKVYKGM LDLLKCTVLS LEQSYAHAGL GGMASIFGLL 1020
EIAQTHYYSK EPDKRKRSPT ENVNTPVGKD PGLAGRGDPK AMAQLRVPQL GPRAPSATGR 1080
GPKELDTRSL KEENFVASVG PEVIKPVFDL GETEEKKSQI SADSGVSLAS ASQRTDQDSV 1140
IGVSPAVMIR SSSQDSEVSN SSGETLGADS DLSSNAGDGP GGEGSAHLAS SRATLSDSEI 1200
ETNSATSTIF GKAHSLKPKE KPASSPVRSS EDVSQRVYLY EGLLGRDKGS MWDQLEDAAM 1260
ETFSISKERS TLWDQMQFWE DAFLDAVMLE REGMGMDQGP QEMIDRYLSL GEHDRKRLED 1320
DEDRLLATLL HNLISYMLLM KVNKNDIRKK VRRLMGKSHV GLVYSQQINE VLDQLTNLNG 1380
RDLSIRSSGS RHMKKQTFVV HAGTDTNGDI FFMEVCDDCV VLRSNIGTVY ERWWYEKLIN 1440
MTYCPKTKVL CLWRRNGSET QLNKFYTKKC RELYYCVKDS MERAAARQQS IKPGPELGGE 1500
FPVQDMKTGE GGLLQVTLEG INLKFMHNQV FIELNHIKKC NTVRGVFVLE EFVPEIKEVV 1560
SHKYKTPMAH EICYSVLCLF SYVAAVRSSE EDLRTPPRPV SS 1603
Keyword

KW-0053--Apoptosis
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0903--Direct protein sequencing
KW-0344--Guanine-nucleotide releasing factor
KW-0472--Membrane
KW-0597--Phosphoprotein
KW-1185--Reference proteome
Interpro

IPR005112--dDENN_dom
IPR001194--DENN_dom
IPR005113--uDENN_dom
PROSITE

PS50947--DDENN
PS50211--DENN
PS50946--UDENN
Pfam

PF02141--DENN
PF03456--uDENN
Gene Ontology

GO:0005829--C:cytosol
GO:0016021--C:integral component of membrane
GO:0005634--C:nucleus
GO:0045202--C:synapse
GO:0008021--C:synaptic vesicle
GO:0017112--F:Rab guanyl-nucleotide exchange factor activity
GO:0000187--P:activation of MAPK activity
GO:0006915--P:apoptotic process
GO:0060125--P:negative regulation of growth hormone secretion
GO:1902277--P:negative regulation of pancreatic amylase secretion
GO:0043547--P:positive regulation of GTPase activity
GO:0043410--P:positive regulation of MAPK cascade
GO:0051726--P:regulation of cell cycle
GO:1902041--P:regulation of extrinsic apoptotic signaling pathway via death domain receptors
GO:0032483--P:regulation of Rab protein signal transduction