dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000366
Uniprot Accession O08796; EF2K_MOUSE;
Genbank Protein ID NP_001254639.1; NP_001254640.1; NP_031934.1; XP_006507405.1; XP_006507406.1;
Genbank Nucleotide ID NM_001267710.1; NM_001267711.1; NM_007908.4; XM_006507342.2; XM_006507343.2;
Protein Name Eukaryotic elongation factor 2 kinase
Protein Synonyms/Alias
Gene Name Eef2k
Gene Synonyms/Alias
Organism Mus musculus(Mouse)
NCBI Taxa ID 10090
Functional Description
(View all)		 Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
Phosphorylation Sites
 dbPAF PTMs: 37 (View all)
PositionPeptidesSourceReferences ( PMIDs )
19GVDGGRCSRAGHNADcurated25266776; 23984901; 25338131
27RAGHNADSDTDSDDDdbPTM 3.0;PhosphoSitePlus;curated19367708; 16381945; 23193290; 21183079; 21659605; 22135298;
29GHNADSDTDSDDDEGdbPTM 3.0;PhosphoSitePlus;curated19367708; 16381945; 23193290; 21183079; 21659605; 22135298;
31NADSDTDSDDDEGYFdbPTM 3.0;PhosphoSitePlus;curated19367708; 16381945; 23193290; 21183079; 21659605; 22135298;
58NVSSKVQSYYSNLTKcurated25338131
66YYSNLTKTECGSTGSPhosphoPep 2.019060867
70LTKTECGSTGSPASSdbPTM 3.0;Phospho.ELM 9.0;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;UniProt;curated17242355; 16381945; 23193290; 21183079; 21659605; 19060867;
71TKTECGSTGSPASSFdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;curated21183079; 16381945; 23193290; 19060867; 22135298; 23684622;
73TECGSTGSPASSFHFdbPTM 3.0;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;curated19367708; 21183079; 21659605; 16381945; 23193290; 17242355;
76GSTGSPASSFHFKEAdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;curated21183079; 16381945; 23193290; 17242355; 19060867; 22135298;
77STGSPASSFHFKEAWdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;UniProt;curated15024086; 21183079; 16381945; 23193290; 19060867; 22135298;
242YIKYNSNSGFVRDDNcurated25338131
347RLLQSAKTILRGTEEdbPTM 3.0;PHOSIDA;PhosphoSitePlus;curated20415495; 21183079; 16381945; 23193290; 21081558; 22135298;
352AKTILRGTEEKCGSPcurated25266776
358GTEEKCGSPRIRTLSPhosphoSitePlus22135298
363CGSPRIRTLSSSRPPPhosphoSitePlus22135298
365SPRIRTLSSSRPPLLdbPTM 3.0;PhosphoSitePlus;curated19367708; 21183079; 16381945; 23193290; 22135298; 22817900;
367RIRTLSSSRPPLLLRdbPTM 3.0;PhosphoSitePlus;curated21183079; 16381945; 23193290; 22135298; 22817900
376PPLLLRLSENSGDENPhosphoPep 2.0;curated19060867; 21183079; 25338131
379LLRLSENSGDENMSDdbPTM 3.0;PhosphoSitePlus21183079; 16381945; 23193290; 22135298
Sequence
(Fasta)		MADEDLIFCL EGVDGGRCSR AGHNADSDTD SDDDEGYFIC PITDDHMSNQ NVSSKVQSYY 60
SNLTKTECGS TGSPASSFHF KEAWKHAIEK AKHMPDPWAE FHLEDIATEH ATRHRYNAVT 120
GEWLKDEVLI KMASQPFGRG AMRECFRTKK LSNFLHAQQW KGASNYVAKR YIEPVDRSVY 180
FEDVQLQMEA KLWGEDYNRH KPPKQVDIMQ MCIIELKDRP GQPLFHLEHY IEGKYIKYNS 240
NSGFVRDDNI RLTPQAFSHF TFERSGHQLI VVDIQGVGDL YTDPQIHTEK GTDFGDGNLG 300
VRGMALFFYS HACNRICQSM GLTPFDLSPR EQDAVNQSTR LLQSAKTILR GTEEKCGSPR 360
IRTLSSSRPP LLLRLSENSG DENMSDVTFD SLPSSPSSAT PHSQKLDHLH WPVFGDLDNM 420
GPRDHDRMDN HRDSENSGDS GYPSEKRSDL DDPEPREHGH SNGNRRHESD EDSLGSSGRV 480
CVETWNLLNP SRLHLPRPSA VALEVQRLNA LDLGRKIGKS VLGKVHLAMV RYHEGGRFCE 540
KDEEWDRESA IFHLEHAADL GELEAIVGLG LMYSQLPHHI LADVSLKETE ENKTKGFDYL 600
LKAAEAGDRH SMILVARAFD TGLNLSPDRC QDWSEALHWY NTALETTDCD EGGEYDGIQD 660
EPQYALLARE AEMLLTGGFG LDKNPQRSGD LYTQAAEAAM EAMKGRLANQ YYEKAEEAWA 720
QMEE 725
Keyword

KW-0007--Acetylation
KW-0067--ATP-binding
KW-0106--Calcium
KW-0112--Calmodulin-binding
KW-0181--Complete proteome
KW-0418--Kinase
KW-0547--Nucleotide-binding
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0723--Serine/threonine-protein kinase
KW-0808--Transferase
Interpro

IPR017400--eEF-2K
IPR011009--Kinase-like_dom
IPR004166--MHCK_EF2_kinase
IPR006597--Sel1-like
IPR011990--TPR-like_helical_dom
PROSITE

PS51158--ALPHA_KINASE
Pfam

PF02816--Alpha_kinase
PF08238--Sel1
Gene Ontology

GO:0005524--F:ATP binding
GO:0005509--F:calcium ion binding
GO:0005516--F:calmodulin binding
GO:0004686--F:elongation factor-2 kinase activity
GO:0008135--F:translation factor activity, RNA binding
GO:0046777--P:protein autophosphorylation
GO:0031952--P:regulation of protein autophosphorylation
GO:0006414--P:translational elongation