dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000336
Uniprot Accession O08715; AKAP1_MOUSE; B1AR25; O08714; P97488;
Genbank Protein ID NP_001036006.1; NP_033778.2;
Genbank Nucleotide ID NM_001042541.1; NM_009648.2;
Protein Name A-kinase anchor protein 1, mitochondrial
Protein Synonyms/Alias Dual specificity A-kinase-anchoring protein 1;D-AKAP-1;Protein kinase A-anchoring protein 1;PRKA1;Spermatid A-kinase anchor protein;S-AKAP;
Gene Name Akap1
Gene Synonyms/Alias Akap;
Organism Mus musculus(Mouse)
NCBI Taxa ID 10090
Functional Description
(View all)		 Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A. Anchors them to the cytoplasmic face of the mitochondrial outer membrane or allows them to reside in the endoplasmic reticulum. Does not contain the classic KDEL endoplasmic reticulum-targeting sequence. This explains how it is able to switch its localization, either being in the endoplasmic reticulum or in the mitochondria depending on which N-terminal part begins the isoform. The longest N-terminal part only present in isoform 2 and isoform 4 acts as a suppressor of mitochondrial targeting and as an activator of recessive endoplasmic reticulum targeting motif.
Phosphorylation Sites
 dbPAF PTMs: 26 (View all)
PositionPeptidesSourceReferences ( PMIDs )
55AIKDRRLSEEACPGVdbPTM 3.0;Phospho.ELM 9.0;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;UniProt;curated17242355; 18630941; 19367708; 16381945; 23193290; 19854140;
64EACPGVLSVAPTVTQPhosphoSitePlus22135298
101TRQVRRRSESSGNLPdbPTM 3.0;Phospho.ELM 9.0;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;UniProt;curated17242355; 16381945; 23193290; 18846507; 19854140; 20139300;
103QVRRRSESSGNLPSVdbPTM 3.0;Phospho.ELM 9.0;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;UniProt;curated17242355; 16381945; 23193290; 19367708; 19854140; 21183079;
104VRRRSESSGNLPSVAdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;curated21659604; 16381945; 23193290; 17242355; 19060867; 22135298;
109ESSGNLPSVADTRSQPhosphoSitePlus;curated22135298; 25266776; 23567750
115PSVADTRSQPGPCRDPhosphoSitePlus22135298
138LMGDKAKSIPLGCPLPhosphoSitePlus;curated22135298; 20469934
151PLLPKDASFPYEAVEdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;curated21183079; 16381945; 23193290; 19060867; 22135298; 23984901
282LEPQVKGSSNTSDRDcurated25338131
283EPQVKGSSNTSDRDLcurated25338131
285QVKGSSNTSDRDLAGcurated25338131
333RATTVGKTVAQVHPTPhosphoSitePlus22135298
378TRTGATASPSAEALPdbPTM 3.0;PhosphoSitePlus;SysPTM 2.0;curated21659604; 16381945; 23193290; 18266315; 22135298; 19366988;
380TGATASPSAEALPPKPhosphoSitePlus22135298
394KTYVSCLSSPLSGPTPhosphoSitePlus;curated22135298; 25266776; 23984901
395TYVSCLSSPLSGPTKdbPTM 3.0;PhosphoSitePlus;curated21659604; 16381945; 23193290; 22135298; 25266776; 25338131
423APCPPPVTPQRQSLEPhosphoSitePlus22135298
428PVTPQRQSLEGASNPcurated25338131
487SSGDKAMTPPLPVSTdbPTM 3.0;PhosphoSitePlus;curated19854140; 21659605; 16381945; 23193290; 22135298; 20469934;
Sequence
(Fasta)		MAIQLRSLFP LALPGMLALL GWWWFFSRKK DRLSSSDKQV ETLKVGPAIK DRRLSEEACP 60
GVLSVAPTVT QPPGREEQRC VDKPSTEPLA LPRTRQVRRR SESSGNLPSV ADTRSQPGPC 120
RDEIAKVELS LMGDKAKSIP LGCPLLPKDA SFPYEAVERC KQESALGKTP GRGWPSPYAA 180
SGEKARETGG TEGTGDAVLG ENVSEEGLLS QECVSEVEKS EFPILAPGGG EGEEVSHGPP 240
QVAELLKKEE YIVGKLPSSF VEPVHSEPVK DEDALEPQVK GSSNTSDRDL AGELDKDETV 300
PENDQIKQAA FQLISQVILE ATEEFRATTV GKTVAQVHPT SATQPKGKEE SCVPASQETS 360
LGQDTSDPAS TRTGATASPS AEALPPKTYV SCLSSPLSGP TKDQKPKNSA HHISLAPCPP 420
PVTPQRQSLE GASNPRGDDN FVACMANNSQ SVLSVSSLGQ CSDPVSTSGL EDSCTETISS 480
SGDKAMTPPL PVSTQPFSNG VLKEELSDLG TEDGWTMDTE ADHSGGSDGN SMDSVDSCCG 540
LTKPDSPQSV QAGSNPKKVD LIIWEIEVPK HLVGRLIGKQ GRYVSFLKQT SGAKIYISTL 600
PYTQNIQICH IEGSQHHVDK ALNLIGKKFK ELNLTNIYAP PLPSLALPSL PMTSWLMLPD 660
GITVEVIVVN QVNAGHLFVQ QHTHPTFHAL RSLDQQMYLC YSQPGIPTLP TPVEITVICA 720
APGADGAWWR AQVVASYEET NEVEIRYVDY GGYKRVKVDV LRQIRSDFVT LPFQGAEVLL 780
DSVVPLSDDD HFSPEADAAM SEMTGNTALL AQVTSYSATG LPLIQLWSVV GDEVVLINRS 840
LVERGLAQWV DSYYASL 858
Keyword

KW-0025--Alternative splicing
KW-0181--Complete proteome
KW-0256--Endoplasmic reticulum
KW-0472--Membrane
KW-0496--Mitochondrion
KW-1000--Mitochondrion outer membrane
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0694--RNA-binding
KW-0809--Transit peptide
KW-0812--Transmembrane
Interpro

IPR004087--KH_dom
IPR004088--KH_dom_type_1
IPR002999--Tudor
PROSITE

PS50084--KH_TYPE_1
PS50304--TUDOR
Pfam

PF00013--KH_1
PF00567--TUDOR
Gene Ontology

GO:0005783--C:endoplasmic reticulum
GO:0016021--C:integral component of membrane
GO:0005811--C:lipid particle
GO:0016020--C:membrane
GO:0030061--C:mitochondrial crista
GO:0005759--C:mitochondrial matrix
GO:0005741--C:mitochondrial outer membrane
GO:0005739--C:mitochondrion
GO:0031594--C:neuromuscular junction
GO:0045211--C:postsynaptic membrane
GO:0044822--F:poly(A) RNA binding
GO:0034237--F:protein kinase A regulatory subunit binding
GO:0071320--P:cellular response to cAMP
GO:0071375--P:cellular response to peptide hormone stimulus
GO:0010614--P:negative regulation of cardiac muscle hypertrophy
GO:0051534--P:negative regulation of NFAT protein import into nucleus
GO:0035308--P:negative regulation of protein dephosphorylation