dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000322
Uniprot Accession O08679; MARK2_RAT;
Genbank Protein ID NP_067731.1;
Genbank Nucleotide ID NM_021699.1;
Protein Name Serine/threonine-protein kinase MARK2
Protein Synonyms/Alias
Gene Name Mark2
Gene Synonyms/Alias
Organism Rattus norvegicus(Rat)
NCBI Taxa ID 10116
Functional Description
(View all)		 Serine/threonine-protein kinase involved in cell polarity and microtubule dynamics regulation. Phosphorylates CRTC2/TORC2, DCX, HDAC7, KIF13B, MAP2, MAP4, MAPT/TAU and RAB11FIP2. Plays a key role in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Regulates epithelial cell polarity by phosphorylating RAB11FIP2. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Regulates axogenesis by phosphorylating KIF13B, promoting interaction between KIF13B and 14-3-3 and inhibiting microtubule-dependent accumulation of KIF13B. Also required for neurite outgrowth and establishment of neuronal polarity. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells.
Phosphorylation Sites
 dbPAF PTMs: 27 (View all)
PositionPeptidesSourceReferences ( PMIDs )
40NMLRGRNSATSADEQPhosphoSitePlus;curated22135298; 25532521
53EQPHIGNYRLLKTIGPhosphoSitePlus22135298
58GNYRLLKTIGKGNFAdbPTM 3.014517247; 16381945; 23193290
91IDKTQLNSSSLQKLFdbPTM 3.0;PhosphoSitePlus17442826; 16381945; 23193290; 22135298
92DKTQLNSSSLQKLFRdbPTM 3.0;PhosphoSitePlus17442826; 16381945; 23193290; 22135298
93KTQLNSSSLQKLFREdbPTM 3.017442826; 16381945; 23193290
208TFGNKLDTFCGSPPYdbPTM 3.0;PhosphoSitePlus;curated14517247; 16381945; 23193290; 22135298; 21630457
212KLDTFCGSPPYAAPEdbPTM 3.0;PhosphoSitePlus14517247; 16257959; 18424437; 16381945; 23193290; 22135298
274YRIPFYMSTDCENLLdbPTM 3.014517247; 16381945; 23193290
275RIPFYMSTDCENLLKdbPTM 3.014517247; 16381945; 23193290
290KFLILNPSKRGTLEQcurated23984901
294LNPSKRGTLEQIMKDdbPTM 3.0;PhosphoSitePlus;curated17442826; 16381945; 23193290; 22135298; 23984901
365YLLLGYKSSELEGDTPhosphoSitePlus22135298
390LTNSSAPSPSHKVQRcurated22276854
408ANPKQRRSSDQAVPAPhosphoSitePlus;UniProt22135298; 22673903
409NPKQRRSSDQAVPAIPhosphoSitePlus;UniProt22135298; 22673903
418QAVPAIPTSNSYSKKcurated23984901
453STAKVPASPLPGLDRPhosphoSitePlus;UniProt;curated22135298; 22673903; 25532521; 22276854
480LSTSTNRSRNSPLLDPhosphoSitePlus22135298
483STNRSRNSPLLDRASPhosphoSitePlus;UniProt;curated22135298; 22673903; 25532521; 23712012; 21630457; 23984901
Sequence
(Fasta)		MSSARTPLPT LNERDTEQPT LGHLDSKPSS KSNMLRGRNS ATSADEQPHI GNYRLLKTIG 60
KGNFAKVKLA RHILTGKEVA VKIIDKTQLN SSSLQKLFRE VRIMKVLNHP NIVKLFEVIE 120
TEKTLYLVME YASGGEVFDY LVAHGRMKEK EARAKFRQIV SAVQYCHQKF IVHRDLKAEN 180
LLLDADMNIK IADFGFSNEF TFGNKLDTFC GSPPYAAPEL FQGKKYDGPE VDVWSLGVIL 240
YTLVSGSLPF DGQNLKELRE RVLRGKYRIP FYMSTDCENL LKKFLILNPS KRGTLEQIMK 300
DRWMNVGHED DELKPYVEPL PDYKDPRRTE LMVSMGYTRE EIQDSLVGQR YNEVMATYLL 360
LGYKSSELEG DTITLKPRPS ADLTNSSAPS PSHKVQRSVS ANPKQRRSSD QAVPAIPTSN 420
SYSKKTQSNN AENKRPEEET GRKASSTAKV PASPLPGLDR KKTTPTPSTN SVLSTSTNRS 480
RNSPLLDRAS LGQASIQNGK DSTAPQRVPV ASPSAHNISS SSGAPDRTNF PRGVSSRSTF 540
HAGQLRQVRD QQNLPFGVTP ASPSGHSQGR RGASGSIFSK FTSKFVRRNL NEPESKDRVE 600
TLRPHVVGGG GTDKEKEEFR EAKPRSLRFT WSMKTTSSME PNEMMREIRK VLDANSCQSE 660
LHERYMLLCV HGTPGHENFV QWEMEVCKLP RLSLNGVRFK RISGTSMAFK NIASKIANEL 720
KL 723
Keyword

KW-0002--3D-structure
KW-0067--ATP-binding
KW-1003--Cell membrane
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0206--Cytoskeleton
KW-0217--Developmental protein
KW-0221--Differentiation
KW-0418--Kinase
KW-0446--Lipid-binding
KW-0460--Magnesium
KW-0472--Membrane
KW-0479--Metal-binding
KW-0547--Nucleotide-binding
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0723--Serine/threonine-protein kinase
KW-0808--Transferase
KW-0879--Wnt signaling pathway
Interpro

IPR028375--KA1/Ssp2_C
IPR001772--KA1_dom
IPR011009--Kinase-like_dom
IPR000719--Prot_kinase_dom
IPR017441--Protein_kinase_ATP_BS
IPR002290--Ser/Thr_dual-sp_kinase
IPR008271--Ser/Thr_kinase_AS
IPR015940--UBA
PROSITE

PS50032--KA1
PS00107--PROTEIN_KINASE_ATP
PS50011--PROTEIN_KINASE_DOM
PS00108--PROTEIN_KINASE_ST
PS50030--UBA
Pfam

PF02149--KA1
PF00069--Pkinase
PF00627--UBA
Gene Ontology

GO:0005884--C:actin filament
GO:0005737--C:cytoplasm
GO:0016328--C:lateral plasma membrane
GO:0016020--C:membrane
GO:0097427--C:microtubule bundle
GO:0005886--C:plasma membrane
GO:0005524--F:ATP binding
GO:0008289--F:lipid binding
GO:0000287--F:magnesium ion binding
GO:0004674--F:protein serine/threonine kinase activity
GO:0050321--F:tau-protein kinase activity
GO:0030010--P:establishment of cell polarity
GO:0045197--P:establishment or maintenance of epithelial cell apical/basal polarity
GO:0035556--P:intracellular signal transduction
GO:0001764--P:neuron migration
GO:0010976--P:positive regulation of neuron projection development
GO:0046777--P:protein autophosphorylation
GO:0006468--P:protein phosphorylation
GO:0050770--P:regulation of axonogenesis
GO:0051493--P:regulation of cytoskeleton organization
GO:0016055--P:Wnt signaling pathway