dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000309
Uniprot Accession O08648; M3K4_MOUSE; O08649; O70124; Q6PDG6;
Genbank Protein ID NP_036078.2; XP_006523392.1;
Genbank Nucleotide ID NM_011948.2; XM_006523329.2;
Protein Name Mitogen-activated protein kinase kinase kinase 4
Protein Synonyms/Alias MAPK/ERK kinase kinase 4;MEK kinase 4;MEKK 4;
Gene Name Map3k4
Gene Synonyms/Alias Mekk4;
Organism Mus musculus(Mouse)
NCBI Taxa ID 10090
Functional Description
(View all)		 Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6.
Phosphorylation Sites
 dbPAF PTMs: 39 (View all)
PositionPeptidesSourceReferences ( PMIDs )
56LGESARKSMESDPEDdbPTM 3.0;PhosphoSitePlus;curated21183079; 16381945; 23193290; 22135298; 25338131
59SARKSMESDPEDFSDdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;curated21183079; 16381945; 23193290; 19060867; 22135298; 25338131
65ESDPEDFSDETNTETdbPTM 3.0;PhosphoSitePlus;curated21183079; 16381945; 23193290; 22135298; 25338131
70DFSDETNTETLYGTScurated21183079
77TETLYGTSPPSTPRQdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;UniProt;curated21183079; 21659605; 16381945; 23193290; 19060867; 22135298;
112NLKEKMNTPSQSPHKdbPTM 3.0;PhosphoSitePlus;curated21659605; 16381945; 23193290; 22135298; 23684622
114KEKMNTPSQSPHKDLcurated25266776; 23684622; 25338131
116KMNTPSQSPHKDLGKdbPTM 3.0;PhosphoSitePlus;curated21659605; 16381945; 23193290; 22135298; 25266776; 23684622
171AGSLPKKSIPDVDLNcurated23984901
207ARTARQTSRTDCPADPhosphoSitePlus;curated22135298; 25338131
232LLKLTSVSKKKDREQdbPTM 3.0;PhosphoSitePlus20139300; 16381945; 23193290; 22135298
424WPVFEIPSPRPSKGYdbPTM 3.0;PHOSIDA;PhosphoSitePlus;curated19854140; 20415495; 21659605; 16381945; 23193290; 21081558;
428EIPSPRPSKGYEPEDdbPTM 3.0;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;curated21183079; 16381945; 23193290; 21081558; 19060867; 22135298;
449VELRELESGTEESDEdbPTM 3.0;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;UniProt;curated19854140; 20531401; 21183079; 21659605; 16381945; 23193290;
451LRELESGTEESDEEPdbPTM 3.0;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;UniProt;curated21183079; 21659605; 16381945; 23193290; 21081558; 19060867;
454LESGTEESDEEPTPSdbPTM 3.0;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;UniProt;curated19854140; 20415495; 20531401; 21183079; 21659605; 16381945;
461SDEEPTPSPRVPELRcurated25338131
477STDAILDSRSQGCVSPhosphoSitePlus22135298
492RKLERLESEEDSIGWdbPTM 3.0;Phospho.ELM 9.0;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;UniProt;curated17242355; 16381945; 23193290; 21183079; 21659605; 19060867;
496RLESEEDSIGWGTADcurated25266776; 25338131
Sequence
(Fasta)		MRDAIAEPVP PPALADTPAA AMEELRPAPP PQPEPDPECC PAARQECMLG ESARKSMESD 60
PEDFSDETNT ETLYGTSPPS TPRQMKRLSA KHQRNSAGRP ASRSNLKEKM NTPSQSPHKD 120
LGKGVETVEE YSYKQEKKIR ATLRTTERDH KKNAQCSFML DSVAGSLPKK SIPDVDLNKP 180
YLSLGCSNAK LPVSMPMPIA RTARQTSRTD CPADRLKFFE TLRLLLKLTS VSKKKDREQR 240
GQENTAAFWF NRSNELIWLE LQAWHAGRTI NDQDLFLYTA RQAIPDIINE ILTFKVNYGS 300
IAFSSNGAGF NGPLVEGQCR TPQETNRVGC SSYHEHLQRQ RVSFEQVKRI MELLEYMEAL 360
YPSLQALQKD YERYAAKDFE DRVQALCLWL NITKDLNQKL RIMGTVLGIK NLSDIGWPVF 420
EIPSPRPSKG YEPEDEVEDT EVELRELESG TEESDEEPTP SPRVPELRLS TDAILDSRSQ 480
GCVSRKLERL ESEEDSIGWG TADCGPEASR HCLTSIYRPF VDKALKQMGL RKLILRLHKL 540
MNGSLQRARV ALVKDDRPVE FSDFPGPMWG SDYVQLSGTP PSSEQKCSAV SWEELRAMDL 600
PSFEPAFLVL CRVLLNVIHE CLKLRLEQRP AGEPSLLSIK QLVRECKEVL KGGLLMKQYY 660
QFMLQEVLGG LEKTDCNMDA FEEDLQKMLM VYFDYMRSWI QMLQQLPQAS HSLKNLLEEE 720
WNFTKEITHY IRGGEAQAGK LFCDIAGMLL KSTGSFLESG LQESCAELWT SADDNGAADE 780
LRRSVIEISR ALKELFHEAR ERASKALGFA KMLRKDLEIA AEFVLSASAR ELLDALKAKQ 840
YVKVQIPGLE NLHVFVPDSL AEEKKIILQL LNAATGKDCS KDPDDVFMDA FLLLTKHGDR 900
ARDSEDGWGT WEARAVKIVP QVETVDTLRS MQVDNLLLVV MESAHLVLQR KAFQQSIEGL 960
MTVRHEQTSS QPIIAKGLQQ LKNDALELCN RISDAIDRVD HMFTLEFDAE VEESESATLQ 1020
QYYREAMIQG YNFGFEYHKE VVRLMSGEFR QKIGDKYISF AQKWMNYVLT KCESGRGTRP 1080
RWATQGFDFL QAIEPAFISA LPEDDFLSLQ ALMNECIGHV IGKPHSPVTA IHRNSPRPVK 1140
VPRCHSDPPN PHLIIPTPEG FSTRSVPSDA RTHGNSVAAA AAVAAAATTA AGRPGPGGGD 1200
SVPAKPVNTA PDTRGSSVPE NDRLASIAAE LQFRSLSRHS SPTEERDEPA YPRSDSSGST 1260
RRSWELRTLI SQTKDSASKQ GPIEAIQKSV RLFEERRYRE MRRKNIIGQV CDTPKSYDNV 1320
MHVGLRKVTF KWQRGNKIGE GQYGKVYTCI SVDTGELMAM KEIRFQPNDH KTIKETADEL 1380
KIFEGIKHPN LVRYFGVELH REEMYIFMEY CDEGTLEEVS RLGLQEHVIR LYTKQITVAI 1440
NVLHEHGIVH RDIKGANIFL TSSGLIKLGD FGCSVKLKNN AQTMPGEVNS TLGTAAYMAP 1500
EVITRAKGEG HGRAADIWSL GCVVIEMVTG KRPWHEYEHN FQIMYKVGMG HKPPIPERLS 1560
PEGKAFLSHC LESDPKIRWT ASQLLDHAFV KVCTDEE 1598
Keyword

KW-0025--Alternative splicing
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0418--Kinase
KW-0460--Magnesium
KW-0479--Metal-binding
KW-0547--Nucleotide-binding
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0723--Serine/threonine-protein kinase
KW-0808--Transferase
Interpro

IPR011009--Kinase-like_dom
IPR000719--Prot_kinase_dom
IPR017441--Protein_kinase_ATP_BS
IPR002290--Ser/Thr_dual-sp_kinase
IPR008271--Ser/Thr_kinase_AS
PROSITE

PS00107--PROTEIN_KINASE_ATP
PS50011--PROTEIN_KINASE_DOM
PS00108--PROTEIN_KINASE_ST
Pfam

PF00069--Pkinase
Gene Ontology

GO:0005737--C:cytoplasm
GO:0005622--C:intracellular
GO:0048471--C:perinuclear region of cytoplasm
GO:0005524--F:ATP binding
GO:0004708--F:MAP kinase kinase activity
GO:0004709--F:MAP kinase kinase kinase activity
GO:0046872--F:metal ion binding
GO:0004672--F:protein kinase activity
GO:0000187--P:activation of MAPK activity
GO:0000186--P:activation of MAPKK activity
GO:0060718--P:chorionic trophoblast cell differentiation
GO:0048263--P:determination of dorsal identity
GO:0035556--P:intracellular signal transduction
GO:0019100--P:male germ-line sex determination
GO:0000165--P:MAPK cascade
GO:0001890--P:placenta development
GO:0043507--P:positive regulation of JUN kinase activity
GO:1900745--P:positive regulation of p38MAPK cascade
GO:0010468--P:regulation of gene expression
GO:0010225--P:response to UV-C