dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000268
Uniprot Accession O08550; KMT2B_MOUSE; E9QKF4; Q5NU09;
Genbank Protein ID NP_083550.2;
Genbank Nucleotide ID NM_029274.2;
Protein Name Histone-lysine N-methyltransferase 2B
Protein Synonyms/Alias
Gene Name Kmt2b
Gene Synonyms/Alias Mll2;Trx2;Wbp7;
Organism Mus musculus(Mouse)
NCBI Taxa ID 10090
Functional Description
(View all)		 Histone methyltransferase. Methylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2 (By similarity). Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development. Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation.
Phosphorylation Sites
 dbPAF PTMs: 52 (View all)
PositionPeptidesSourceReferences ( PMIDs )
113GCMPEEESSDGESEEdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;UniProt;curated21183079; 16381945; 23193290; 19060867; 22135298
114CMPEEESSDGESEEEdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;UniProt;curated21183079; 16381945; 23193290; 19060867; 22135298
118EESSDGESEEEEFQGdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;UniProt;curated21183079; 16381945; 23193290; 19060867; 22135298
185RKRGEEGTERMVQALdbPTM 3.0;PhosphoSitePlus;curated21183079; 16381945; 23193290; 22135298
216ARAREPSTPRRSRGRcurated23384938
320KVKMGQLSQELESGQdbPTM 3.017525332; 16381945; 23193290
467APEKQEESPPLVPATdbPTM 3.0;PhosphoSitePlus;curated19854140; 16381945; 23193290; 22135298; 25266776; 25338131
504SGPEGTLSPTPNPSTcurated25338131
515NPSTTTGSPLEDSPTcurated25338131
579VRPPVATSPPAPQEPdbPTM 3.0;PhosphoSitePlus;curated21183079; 21659605; 16381945; 23193290; 22135298; 25338131
590PQEPVPVSSPPRVPTdbPTM 3.0;PhosphoSitePlus;curated21183079; 16381945; 23193290; 22135298
591QEPVPVSSPPRVPTPPhosphoSitePlus;curated22135298; 25338131
597SSPPRVPTPPSTPVPdbPTM 3.0;PhosphoSitePlus;curated21183079; 21659605; 16381945; 23193290; 22135298; 25266776;
639PAPPPAPSPPPAPATdbPTM 3.0;PhosphoSitePlus;curated19854140; 16381945; 23193290; 22135298; 25266776
720RFVPVVTSPVKVEVPcurated25338131
784LEKARVASLGSLPLSdbPTM 3.0;PhosphoSitePlus;curated20139300; 16381945; 23193290; 22135298; 25266776
787ARVASLGSLPLSGVEdbPTM 3.0;PhosphoSitePlus20139300; 16381945; 23193290; 22135298
822QQQKVAASMPLSPAVPhosphoSitePlus22135298
826VAASMPLSPAVQTEEdbPTM 3.0;PhosphoSitePlus;curated19854140; 16381945; 23193290; 22135298; 25266776; 25338131
849PDRGCVRSEDESMEAdbPTM 3.0;PhosphoSitePlus;curated19366988; 16381945; 23193290; 22135298; 25266776; 23684622;
Sequence
(Fasta)		MAAAAGGGSC PGPGSARGRF PGRPRGSGGG GGRGGRGNGA ERVRVALRRG GGAAGPGGAE 60
PGEDTALLRL LGLRRGLRRL RRLWAGARVQ RGRGRGRGRG WGPNRGCMPE EESSDGESEE 120
EEFQGFHSDE DVAPSSLRSA LRSQRGRAPR GRGRKHKTTP LPPRLADVTP VPPKAPTRKR 180
GEEGTERMVQ ALTELLRRSQ APQPPRSRAR AREPSTPRRS RGRPPGRPAG PCRKKQQAVV 240
LAEAAVTIPK PEPPPPVVPV KNKAGSWKCK EGPGPGPGTP KRGGQPGRGG RGGRGRGRGG 300
LPLMIKFVSK AKKVKMGQLS QELESGQGHG QRGESWQDAP QRKDGDEPER GSCRKKQEQK 360
LEEEEEEEEK EGEEKEEKDD NEDNNKQEEE EETERAVAEE EAMLAKEKEE AKLPSPPLTP 420
PVPSPPPPLP PPSTSPPPPA SPLPPPVSPP PPLSPPPYPA PEKQEESPPL VPATCSRKRG 480
RPPLTPSQRA EREAARSGPE GTLSPTPNPS TTTGSPLEDS PTVVPKSTTF LKNIRQFIMP 540
VVSARSSRVI KTPRRFMDED PPKPPKVEAS IVRPPVATSP PAPQEPVPVS SPPRVPTPPS 600
TPVPLPEKRR SILREPTFRW TSLTRELPPP PPAPPPAPSP PPAPATPSRR PLLLRAPQFT 660
PSEAHLKIYE SVLTPPPLGA LETPEPELPP ADDSPAEPEP RAVGRTNHLS LPRFVPVVTS 720
PVKVEVPPHG APALSEGQQL QLQQPPQALQ TQLLPQALPP QQPQAQPPPS PQHTPPLEKA 780
RVASLGSLPL SGVEEKMFSL LKRAKVQLFK IDQQQQQKVA ASMPLSPAVQ TEEAVGTVKQ 840
TPDRGCVRSE DESMEAKRDR ASGPESPLQG PRIKHVCRHA AVALGQARAM VPEDVPRLSA 900
LPLRDRQDLA TEDTSSASET ESVPSRSQRE KVESAGPGGD SEPTGSTGAL AHTPRRSLPS 960
HHGKKMRMAR CGHCRGCLRV QDCGSCVNCL DKPKFGGPNT KKQCCVYRKC DKIEARKMER 1020
LAKKGRTIVK TLLPWDSDES PEASPGPPGP RRGAGAGGSR EEVGATPGPE EQDSLLLQRK 1080
SARRCVKQRP SYDVFEDSDD SEPGGPPAPR RRTPREHELP VLEPEEQSRP RKPTLQPVLQ 1140
LKARRRLDKD ALAPGPFASF PNGWTGKQKS PDGVHRVRVD FKEDCDLENV WLMGGLSVLT 1200
SVPGGPPMVC LLCASKGLHE LVFCQVCCDP FHPFCLEEAE RPSPQHRDTW CCRRCKFCHV 1260
CGRKGRGSKH LLECERCRHA YHPACLGPSY PTRATRRRRH WICSACVRCK SCGATPGKNW 1320
DVEWSGDYSL CPRCTELYEK GNYCPICTRC YEDNDYESKM MQCAQCDHWV HAKCEGLSDE 1380
DYEILSGLPD SVLYTCGPCA GATQPRWREA LSGALQGGLR QVLQGLLSSK VAGPLLLCTQ 1440
CGQDGKQLHP GPCDLQAVGK RFEEGLYKSV HSFMEDVVAI LMRHSEEGET PERRAGSQMK 1500
GLLLKLLESA FCWFDAHDPK YWRRSTRLPN GVLPNAVLPP SLDHVYAQWR QQESETPESG 1560
QPPGDPSAAF QSKDPAAFSH LDDPRQCALC LKYGDADSKE AGRLLYIGQN EWTHVNCAIW 1620
SAEVFEENDG SLKNVHAAVA RGRQMRCELC LKPGATVGCC LSSCLSNFHF MCARASYCIF 1680
QDDKKVFCQK HTDLLDGKEI VTPDGFDVLR RVYVDFEGIN FKRKFLTGLE PDVINVLIGS 1740
IRINSLGTLS DLSDCEGRLF PIGYQCSRLY WSTVDARRRC WYRCRILEYR PWGPREEPVH 1800
LEAAEENQTI VHSPTPSSDT DSLIPGDPVH HSPIQNLDPP LRTDSSNGPP PTPRSFSGAR 1860
IKVPNYSPSR RPLGGVSFGP LPSPGSPSSL THHIPTVGDS DFPAPPRRSR RPSPLATRPP 1920
PSRRTSSPLR TSPQLRVPLS TSVTALTPTS GELAPPDLAP SPLPPSEDLG PDFEDMEVVS 1980
GLSAADLDFA ASLLGTEPFQ EEIVAAGAVG SSQGGPGDSS EEEASPTTHY VHFPVTVVSG 2040
PALAPSSLAG APRIEQLDGV DDGTDSEAEA VQQPRGQGTP PSGPGVGRGG VLGAAGDRAQ 2100
PPEDLPSEIV DFVLKNLGGP GEGAAGPRED SLPSAPPLAN GSQPPQSLST SPADPTRTFA 2160
WLPGAPGVRV LSLGPAPEPP KPATSKIILV NKLGQVFVKM AGEGEPVAPP VKQPPLPPII 2220
PPTAPTSWTL PPGPLLSVLP VVGVGVVRPA PPPPPPPLTL VFSSGPPSPP RQAIRVKRVS 2280
TFSGRSPPVP PPNKTPRLDE DGESLEDAHH VPGISGSGFS RVRMKTPTVR GVLDLNNPGE 2340
QPEEESPGRP QDRCPLLPLA EAPSQALDGS SDLLFESQWH HYSAGEASSS EEEPPSPEDK 2400
ENQVPKRVGP HLRFEISSDD GFSVEAESLE VAWRTLIEKV QEARGHARLR HLSFSGMSGA 2460
RLLGIHHDAV IFLAEQLPGA QRCQHYKFRY HQQGEGQEEP PLNPHGAARA EVYLRKCTFD 2520
MFNFLASQHR VLPEGATCDE EEDEVQLRST RRATSLELPM AMRFRHLKKT SKEAVGVYRS 2580
AIHGRGLFCK RNIDAGEMVI EYSGIVIRSV LTDKREKFYD GKGIGCYMFR MDDFDVVDAT 2640
MHGNAARFIN HSCEPNCFSR VIHVEGQKHI VIFALRRILR GEELTYDYKF PIEDASNKLP 2700
CNCGAKRCRR FLN 2714
Keyword

KW-0007--Acetylation
KW-0156--Chromatin regulator
KW-0181--Complete proteome
KW-0238--DNA-binding
KW-0479--Metal-binding
KW-0489--Methyltransferase
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0949--S-adenosyl-L-methionine
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0808--Transferase
KW-0862--Zinc
KW-0863--Zinc-finger
Interpro

IPR017956--AT_hook_DNA-bd_motif
IPR003889--FYrich_C
IPR003888--FYrich_N
IPR003616--Post-SET_dom
IPR001214--SET_dom
IPR002857--Znf_CXXC
IPR011011--Znf_FYVE_PHD
IPR001965--Znf_PHD
IPR019787--Znf_PHD-finger
IPR013083--Znf_RING/FYVE/PHD
PROSITE

PS51543--FYRC
PS51542--FYRN
PS50868--POST_SET
PS50280--SET
PS51058--ZF_CXXC
PS01359--ZF_PHD_1
PS50016--ZF_PHD_2
Pfam

PF05965--FYRC
PF05964--FYRN
PF00628--PHD
PF00856--SET
PF02008--zf-CXXC
Gene Ontology

GO:0035097--C:histone methyltransferase complex
GO:0005634--C:nucleus
GO:0003677--F:DNA binding
GO:0042800--F:histone methyltransferase activity (H3-K4 specific)
GO:0008270--F:zinc ion binding
GO:0016458--P:gene silencing
GO:0051568--P:histone H3-K4 methylation
GO:0080182--P:histone H3-K4 trimethylation
GO:0034968--P:histone lysine methylation
GO:0007613--P:memory
GO:0009994--P:oocyte differentiation
GO:0001541--P:ovarian follicle development
GO:0030728--P:ovulation
GO:0051569--P:regulation of histone H3-K4 methylation
GO:0006355--P:regulation of transcription, DNA-templated
GO:0006351--P:transcription, DNA-templated