dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000176
Uniprot Accession O01541; SYAC_CAEEL; Q17371;
Genbank Protein ID NP_491281.1;
Genbank Nucleotide ID NM_058880.3;
Protein Name Alanine--tRNA ligase, cytoplasmic
Protein Synonyms/Alias AlaRS A;Alanyl-tRNA synthetase;
Gene Name aars-2
Gene Synonyms/Alias ars-2;F28H1.3;
Organism Caenorhabditis elegans
NCBI Taxa ID 6239
Functional Description
(View all)		 Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
Phosphorylation Sites
 dbPAF PTMs: 2
PositionPeptidesSourceReferences ( PMIDs )
607QLIMKNHTGTHVLNYPhospho.ELM 9.0;PHOSIDA;curated19530675; 21081558; 18806794
614TGTHVLNYALRKVLAPhospho.ELM 9.0;PHOSIDA19530675; 21081558
Sequence
(Fasta)		MKHLTASEVR STFINFFREK KEHTYVHSSS VIPHDDPTLL FANAGMNQFK PLFLGIADPN 60
SDLAKLKRAV NTQKCIRAGG KHNDLDDVGK DVYHHTYFEM LGNWSFGDYF KKEIITWAWE 120
LLTTVYGIPA ERLYVSVFGG DEANGVPADS EARDIWRSVG VPDERILNFG MKDNFWEMGD 180
VGPCGPCSEI HYDRIGNRDA SHLVNADDPM VVEIWNLVFI QFNREEGGVL KPLPAKHIDC 240
GLGLERLIAV MQDKTSNYDT DIFQPIFEAI HKGSGVRAYT GFIGDEDKDG VDMAYRVVAD 300
HVRTLTIALS DGGRPDNSGR GYVLRRILRR GVRYASEKLN AQPGFFASLV PVVISILGET 360
FPELSRDPVT VMDIINDEEK QFLKTLSRGR VLFQRAVQSL PEGTMTFPGD VSWRLYDTYG 420
FPADLTQLMA EEKGLSVDNT AFEEARRKAI ETSSAGTGKF RDTLDLDVHA LAELQQKGVP 480
TTDDSPKYAY TFTGEGSDAV YKFEPCVGKI LAIRRDGKFV DQLAAGEEGA ILLDRTNFYA 540
EQGGQIYDVG VLTKVNDESN EFNVSNCQVR GGYIVLVGSA EGSFSVGDQV NERFDEDRKQ 600
LIMKNHTGTH VLNYALRKVL ADSDQKGSLV APDRMRFDFT NKAGMTVQQV KKAEEYAQQL 660
IDTKGQVYAK NSPLGEAKKV KGLRAMFDET YPDPVRVVAV GTPVEQLLQN PDAEEGQNTT 720
VEFCGGTHLQ NVSHIGRIVI ASEEAIAKGI RRIVALTGPE AERAIARADR LTARLEEESK 780
HADKKDELLA NKDKFKALQK KIQEIVDEAN GAQLPYWRKD SIREKAKAIQ KTLDGYTKAQ 840
QAAVAEKVLG EAKELAAVAE QPTVLVHVFA ANANSKAIDN ALKLLKDTKA VMAFSVNEDS 900
GKVLCLAKVD KSLVSNGLKA NEWVNEVCTV LGGKGGGKDA NAQLTGENVD KLDAAVELAQ 960
KFALAAIN 969
Keyword

KW-0030--Aminoacyl-tRNA synthetase
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0436--Ligase
KW-0479--Metal-binding
KW-0547--Nucleotide-binding
KW-0648--Protein biosynthesis
KW-1185--Reference proteome
KW-0694--RNA-binding
KW-0820--tRNA-binding
KW-0862--Zinc
Interpro

IPR002318--Ala-tRNA-lgiase_IIc
IPR018162--Ala-tRNA-ligase_IIc_anticod-bd
IPR018165--Ala-tRNA-synth_IIc_core
IPR018164--Ala-tRNA-synth_IIc_N
IPR023033--Ala_tRNA_ligase_euk/bac
IPR003156--DHHA1_dom
IPR018163--Thr/Ala-tRNA-synth_IIc_edit
IPR009000--Transl_B-barrel
IPR012947--tRNA_SAD
PROSITE

PS50860--AA_TRNA_LIGASE_II_ALA
Pfam

PF02272--DHHA1
PF01411--tRNA-synt_2c
PF07973--tRNA_SAD
Gene Ontology

GO:0005829--C:cytosol
GO:0004813--F:alanine-tRNA ligase activity
GO:0016597--F:amino acid binding
GO:0005524--F:ATP binding
GO:0046872--F:metal ion binding
GO:0000049--F:tRNA binding
GO:0006419--P:alanyl-tRNA aminoacylation
GO:0006400--P:tRNA modification