Tag |
Content |
dbPAF ID |
dbPAF-0000153 |
Uniprot Accession |
O00746; NDKM_HUMAN; A2IDD0; Q5U0M9; |
Genbank Protein ID |
NP_001273362.1; NP_001273364.1; NP_001273365.1; NP_001273367.1; NP_001273368.1; NP_001273369.1; NP_005000.1; |
Genbank Nucleotide ID |
NM_001286433.1; NM_001286435.1; NM_001286436.1; NM_001286438.1; NM_001286439.1; NM_001286440.1; NM_005009.2; |
Protein Name |
Nucleoside diphosphate kinase, mitochondrial |
Protein Synonyms/Alias |
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Gene Name |
NME4 |
Gene Synonyms/Alias |
NM23D; |
Organism |
Homo sapiens(Human) |
NCBI Taxa ID |
9606 |
Functional Description (View all) |
Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Through the catalyzed exchange of gamma-phosphate between di- and triphosphonucleosides participates in regulation of intracellular nucleotide homeostasis (PubMed:10799505). Binds to anionic phospholipids, predominantly to cardiolipin; the binding inhibits its phosphotransfer activity (PubMed:18635542, PubMed:23150663). Acts as mitochondria-specific NDK; its association with cardiolipin-containing mitochondrial inner membrane is coupled to respiration suggesting that ADP locally regenerated in the mitochondrion innermembrane space by its activity is directly taken up via ANT ADP/ATP translocase into the matrix space to stimulate respiratory ATP regeneration (PubMed:18635542). Proposed to increase GTP-loading on dynamin-related GTPase OPA1 in mitochondria (PubMed:24970086). In vitro can induce liposome cross-linking suggesting that it can cross-link inner and outer membranes to form contact sites, and promotes intermembrane migration of anionic phosphoplipids. Promotes the redistribution of cardiolipin between the mitochondrial inner membrane and outer membrane which is implicated in pro-apoptotic signaling (PubMed:18635542, PubMed:17028143, PubMed:23150663).Functional Description
Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Through the catalyzed exchange of gamma-phosphate between di- and triphosphonucleosides participates in regulation of intracellular nucleotide homeostasis (PubMed:10799505). Binds to anionic phospholipids, predominantly to cardiolipin; the binding inhibits its phosphotransfer activity (PubMed:18635542, PubMed:23150663). Acts as mitochondria-specific NDK; its association with cardiolipin-containing mitochondrial inner membrane is coupled to respiration suggesting that ADP locally regenerated in the mitochondrion innermembrane space by its activity is directly taken up via ANT ADP/ATP translocase into the matrix space to stimulate respiratory ATP regeneration (PubMed:18635542). Proposed to increase GTP-loading on dynamin-related GTPase OPA1 in mitochondria (PubMed:24970086). In vitro can induce liposome cross-linking suggesting that it can cross-link inner and outer membranes to form contact sites, and promotes intermembrane migration of anionic phosphoplipids. Promotes the redistribution of cardiolipin between the mitochondrial inner membrane and outer membrane which is implicated in pro-apoptotic signaling (PubMed:18635542, PubMed:17028143, PubMed:23150663).
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Phosphorylation Sites
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dbPAF PTMs: 7 |
Sequence (Fasta) | MGGLFWRSAL RGLRCGPRAP GPSLLVRHGS GGPSWTRERT LVAVKPDGVQ RRLVGDVIQR 60 FERRGFTLVG MKMLQAPESV LAEHYQDLRR KPFYPALIRY MSSGPVVAMV WEGYNVVRAS 120 RAMIGHTDSA EAAPGTIRGD FSVHISRNVI HASDSVEGAQ REIQLWFQSS ELVSWADGGQ 180 HSSIHPA
188Fasta Sequence
>O00746|NME4|Homo sapiens(Human) MGGLFWRSALRGLRCGPRAPGPSLLVRHGSGGPSWTRERTLVAVKPDGVQRRLVGDVIQRFERRGFTLVGMKMLQAPESVLAEHYQDLRRKPFYPALIRYMSSGPVVAMVWEGYNVVRASRAMIGHTDSAEAAPGTIRGDFSVHISRNVIHASDSVEGAQREIQLWFQSSELVSWADGGQHSSIHPA
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Keyword |
KW-0002--3D-structure KW-0025--Alternative splicing KW-0067--ATP-binding KW-0181--Complete proteome KW-0903--Direct protein sequencing KW-0418--Kinase KW-0445--Lipid transport KW-0446--Lipid-binding KW-0460--Magnesium KW-0472--Membrane KW-0479--Metal-binding KW-0496--Mitochondrion KW-0546--Nucleotide metabolism KW-0547--Nucleotide-binding KW-1185--Reference proteome KW-0808--Transferase KW-0809--Transit peptide KW-0813--Transport
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Interpro |
IPR001564--Nucleoside_diP_kinase IPR023005--Nucleoside_diP_kinase_AS
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PROSITE |
PS00469--NDP_KINASES
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Pfam |
PF00334--NDK
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Gene Ontology |
GO:0005829--C:cytosol GO:0005622--C:intracellular GO:0005743--C:mitochondrial inner membrane GO:0005758--C:mitochondrial intermembrane space GO:0005759--C:mitochondrial matrix GO:0005739--C:mitochondrion GO:0005524--F:ATP binding GO:0008289--F:lipid binding GO:0046872--F:metal ion binding GO:0004550--F:nucleoside diphosphate kinase activity GO:0006241--P:CTP biosynthetic process GO:0006183--P:GTP biosynthetic process GO:0006869--P:lipid transport GO:0015949--P:nucleobase-containing small molecule interconversion GO:0055086--P:nucleobase-containing small molecule metabolic process GO:0009116--P:nucleoside metabolic process GO:0009142--P:nucleoside triphosphate biosynthetic process GO:0006163--P:purine nucleotide metabolic process GO:0006220--P:pyrimidine nucleotide metabolic process GO:0042981--P:regulation of apoptotic process GO:0044281--P:small molecule metabolic process GO:0006228--P:UTP biosynthetic process
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