dbPAF Protein Information


Tag Content
dbPAF ID dbPAF-0000112
Uniprot Accession O00487; PSDE_HUMAN; B3KNW2; O00176;
Genbank Protein ID NP_005796.1;
Genbank Nucleotide ID NM_005805.5;
Protein Name 26S proteasome non-ATPase regulatory subunit 14
Protein Synonyms/Alias 26S proteasome regulatory subunit RPN11;26S proteasome-associated PAD1 homolog 1;
Gene Name PSMD14
Gene Synonyms/Alias POH1;
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)
Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. Plays a role in response to double-strand breaks (DSBs): acts as a regulator of non-homologous end joining (NHEJ) by cleaving 'Lys-63'-linked polyubiquitin, thereby promoting retention of JMJD2A/KDM4A on chromatin and restricting TP53BP1 accumulation. Also involved in homologous recombination repair by promoting RAD51 loading.
Phosphorylation Sites
dbPAF PTMs: 8
PositionPeptidesSourceReferences ( PMIDs )
32VDTAEQVYISSLALLdbPTM 3.0;HPRD 9;PhosphoPep 2.0;PhosphoSitePlus;curated15592455; 16381945; 23193290; 18083107; 18180459; 19690332;
34TAEQVYISSLALLKMPhosphoSitePlus22135298
150VVVDPIQSVKGKVVIdbPTM 3.0;HPRD 9;PhosphoPep 2.0;PhosphoSitePlus;UniProt17693683; 16381945; 23193290; 18988627; 19060867; 22135298;
177LGHEPRQTTSNLGHLdbPTM 3.0;HPRD 9;curated20068231; 16381945; 23193290; 18988627
179HEPRQTTSNLGHLNKdbPTM 3.0;HPRD 9;curated20068231; 16381945; 23193290; 18988627
224LLNLHKKSWMEGLTLdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;UniProt;curated17323924; 16381945; 23193290; 19060867; 22135298; 23898821;
234EGLTLQDYSEHCKHNPhosphoSitePlus22135298
266VEEEDKMTPEQLAIKPhosphoSitePlus;curated22135298; 25627689; 23917254
Sequence
(Fasta)
MDRLLRLGGG MPGLGQGPPT DAPAVDTAEQ VYISSLALLK MLKHGRAGVP MEVMGLMLGE 60
FVDDYTVRVI DVFAMPQSGT GVSVEAVDPV FQAKMLDMLK QTGRPEMVVG WYHSHPGFGC 120
WLSGVDINTQ QSFEALSERA VAVVVDPIQS VKGKVVIDAF RLINANMMVL GHEPRQTTSN 180
LGHLNKPSIQ ALIHGLNRHY YSITINYRKN ELEQKMLLNL HKKSWMEGLT LQDYSEHCKH 240
NESVVKEMLE LAKNYNKAVE EEDKMTPEQL AIKNVGKQDP KRHLEEHVDV LMTSNIVQCL 300
AAMLDTVVFK 311
Keyword

KW-0181--Complete proteome
KW-0903--Direct protein sequencing
KW-0227--DNA damage
KW-0234--DNA repair
KW-0378--Hydrolase
KW-0479--Metal-binding
KW-0482--Metalloprotease
KW-0597--Phosphoprotein
KW-0645--Protease
KW-0647--Proteasome
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway
KW-0862--Zinc

Interpro

IPR000555--JAMM/MPN+_dom
IPR024969--Rpn11/EIF3F_C

PROSITE
Pfam

PF01398--JAB
PF13012--MitMem_reg

Gene Ontology

GO:0005829--C:cytosol
GO:0031597--C:cytosolic proteasome complex
GO:0070062--C:extracellular exosome
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0022624--C:proteasome accessory complex
GO:0000502--C:proteasome complex
GO:0008541--C:proteasome regulatory particle, lid subcomplex
GO:0061133--F:endopeptidase activator activity
GO:0046872--F:metal ion binding
GO:0008237--F:metallopeptidase activity
GO:0070628--F:proteasome binding
GO:0000186--P:activation of MAPKK activity
GO:0031145--P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0042590--P:antigen processing and presentation of exogenous peptide antigen via MHC class I
GO:0002479--P:antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
GO:0002474--P:antigen processing and presentation of peptide antigen via MHC class I
GO:0006915--P:apoptotic process
GO:0007411--P:axon guidance
GO:0034641--P:cellular nitrogen compound metabolic process
GO:0006977--P:DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
GO:0000724--P:double-strand break repair via homologous recombination
GO:0006303--P:double-strand break repair via nonhomologous end joining
GO:0007173--P:epidermal growth factor receptor signaling pathway
GO:0038095--P:Fc-epsilon receptor signaling pathway
GO:0008543--P:fibroblast growth factor receptor signaling pathway
GO:0000082--P:G1/S transition of mitotic cell cycle
GO:0010467--P:gene expression
GO:0045087--P:innate immune response
GO:0008286--P:insulin receptor signaling pathway
GO:0000165--P:MAPK cascade
GO:0000278--P:mitotic cell cycle
GO:0043066--P:negative regulation of apoptotic process
GO:0090090--P:negative regulation of canonical Wnt signaling pathway
GO:0051436--P:negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
GO:0048011--P:neurotrophin TRK receptor signaling pathway
GO:0038061--P:NIK/NF-kappaB signaling
GO:0006595--P:polyamine metabolic process
GO:0090263--P:positive regulation of canonical Wnt signaling pathway
GO:0010950--P:positive regulation of endopeptidase activity
GO:0051437--P:positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
GO:0012501--P:programmed cell death
GO:0070536--P:protein K63-linked deubiquitination
GO:0000209--P:protein polyubiquitination
GO:0007265--P:Ras protein signal transduction
GO:0042981--P:regulation of apoptotic process
GO:0006521--P:regulation of cellular amino acid metabolic process
GO:0043488--P:regulation of mRNA stability
GO:0061136--P:regulation of proteasomal protein catabolic process
GO:0051439--P:regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
GO:0045471--P:response to ethanol
GO:0007264--P:small GTPase mediated signal transduction
GO:0044281--P:small molecule metabolic process
GO:0002223--P:stimulatory C-type lectin receptor signaling pathway
GO:0050852--P:T cell receptor signaling pathway
GO:0033209--P:tumor necrosis factor-mediated signaling pathway
GO:0006511--P:ubiquitin-dependent protein catabolic process
GO:0048010--P:vascular endothelial growth factor receptor signaling pathway
GO:0016032--P:viral process