dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000101
Uniprot Accession O00468; AGRIN_HUMAN; Q5SVA1; Q5SVA2; Q60FE1; Q7KYS8; Q8N4J5; Q96IC1; Q9BTD4;
Genbank Protein ID NP_940978.2; XP_005244806.1;
Genbank Nucleotide ID NM_198576.3; XM_005244749.2;
Protein Name Agrin
Protein Synonyms/Alias
Gene Name AGRN
Gene Synonyms/Alias AGRIN;
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)		 Isoform 1: heparan sulfate basal lamina glycoprotein that plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ) and directs key events in postsynaptic differentiation. Component of the AGRN-LRP4 receptor complex that induces the phosphorylation and activation of MUSK. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Calcium ions are required for maximal AChR clustering. AGRN function in neurons is highly regulated by alternative splicing, glycan binding and proteolytic processing. Modulates calcium ion homeostasis in neurons, specifically by inducing an increase in cytoplasmic calcium ions. Functions differentially in the central nervous system (CNS) by inhibiting the alpha(3)-subtype of Na+/K+-ATPase and evoking depolarization at CNS synapses. This secreted isoform forms a bridge, after release from motor neurons, to basal lamina through binding laminin via the NtA domain.
Phosphorylation Sites
 dbPAF PTMs: 19
PositionPeptidesSourceReferences ( PMIDs )
123FVNPAPPYLWPAHKNPhosphoSitePlus22135298
200ASCVCKKSPCPSVVAPhosphoSitePlus22135298
204CKKSPCPSVVAPVCGPhosphoSitePlus22135298
217CGSDASTYSNECELQPhosphoSitePlus22135298
218GSDASTYSNECELQRPhosphoSitePlus22135298
257NVTCSFGSTCARSADPhosphoSitePlus22135298
258VTCSFGSTCARSADGPhosphoSitePlus22135298
896AGCEADASAPATCAEPhosphoSitePlus22135298
1159PEMADPKSELFGETAPhosphoSitePlus22135298
1165KSELFGETARSIESTPhosphoSitePlus22135298
1274AGATARATTASRLPSPhosphoSitePlus22135298
1275GATARATTASRLPSSPhosphoSitePlus22135298
1277TARATTASRLPSSAVPhosphoSitePlus22135298
1281TTASRLPSSAVTPRAPhosphoSitePlus22135298
1282TASRLPSSAVTPRAPPhosphoSitePlus22135298
1510AAVALERTFVGAGLRPhosphoSitePlus22135298
1540GIGPGAATRGSGVGEdbPTM 3.0;PhosphoSitePlus;curated21406692; 16381945; 23193290; 22135298; 22817900
1720IRSREPVTLGAWTRVPhosphoSitePlus22135298
1725PVTLGAWTRVSLERNPhosphoSitePlus22135298
Sequence
(Fasta)		MAGRSHPGPL RPLLPLLVVA ACVLPGAGGT CPERALERRE EEANVVLTGT VEEILNVDPV 60
QHTYSCKVRV WRYLKGKDLV ARESLLDGGN KVVISGFGDP LICDNQVSTG DTRIFFVNPA 120
PPYLWPAHKN ELMLNSSLMR ITLRNLEEVE FCVEDKPGTH FTPVPPTPPD ACRGMLCGFG 180
AVCEPNAEGP GRASCVCKKS PCPSVVAPVC GSDASTYSNE CELQRAQCSQ QRRIRLLSRG 240
PCGSRDPCSN VTCSFGSTCA RSADGLTASC LCPATCRGAP EGTVCGSDGA DYPGECQLLR 300
RACARQENVF KKFDGPCDPC QGALPDPSRS CRVNPRTRRP EMLLRPESCP ARQAPVCGDD 360
GVTYENDCVM GRSGAARGLL LQKVRSGQCQ GRDQCPEPCR FNAVCLSRRG RPRCSCDRVT 420
CDGAYRPVCA QDGRTYDSDC WRQQAECRQQ RAIPSKHQGP CDQAPSPCLG VQCAFGATCA 480
VKNGQAACEC LQACSSLYDP VCGSDGVTYG SACELEATAC TLGREIQVAR KGPCDRCGQC 540
RFGALCEAET GRCVCPSECV ALAQPVCGSD GHTYPSECML HVHACTHQIS LHVASAGPCE 600
TCGDAVCAFG AVCSAGQCVC PRCEHPPPGP VCGSDGVTYG SACELREAAC LQQTQIEEAR 660
AGPCEQAECG SGGSGSGEDG DCEQELCRQR GGIWDEDSED GPCVCDFSCQ SVPGSPVCGS 720
DGVTYSTECE LKKARCESQR GLYVAAQGAC RGPTFAPLPP VAPLHCAQTP YGCCQDNITA 780
ARGVGLAGCP SACQCNPHGS YGGTCDPATG QCSCRPGVGG LRCDRCEPGF WNFRGIVTDG 840
RSGCTPCSCD PQGAVRDDCE QMTGLCSCKP GVAGPKCGQC PDGRALGPAG CEADASAPAT 900
CAEMRCEFGA RCVEESGSAH CVCPMLTCPE ANATKVCGSD GVTYGNECQL KTIACRQGLQ 960
ISIQSLGPCQ EAVAPSTHPT SASVTVTTPG LLLSQALPAP PGALPLAPSS TAHSQTTPPP 1020
SSRPRTTASV PRTTVWPVLT VPPTAPSPAP SLVASAFGES GSTDGSSDEE LSGDQEASGG 1080
GSGGLEPLEG SSVATPGPPV ERASCYNSAL GCCSDGKTPS LDAEGSNCPA TKVFQGVLEL 1140
EGVEGQELFY TPEMADPKSE LFGETARSIE STLDDLFRNS DVKKDFRSVR LRDLGPGKSV 1200
RAIVDVHFDP TTAFRAPDVA RALLRQIQVS RRRSLGVRRP LQEHVRFMDF DWFPAFITGA 1260
TSGAIAAGAT ARATTASRLP SSAVTPRAPH PSHTSQPVAK TTAAPTTRRP PTTAPSRVPG 1320
RRPPAPQQPP KPCDSQPCFH GGTCQDWALG GGFTCSCPAG RGGAVCEKVL GAPVPAFEGR 1380
SFLAFPTLRA YHTLRLALEF RALEPQGLLL YNGNARGKDF LALALLDGRV QLRFDTGSGP 1440
AVLTSAVPVE PGQWHRLELS RHWRRGTLSV DGETPVLGES PSGTDGLNLD TDLFVGGVPE 1500
DQAAVALERT FVGAGLRGCI RLLDVNNQRL ELGIGPGAAT RGSGVGECGD HPCLPNPCHG 1560
GAPCQNLEAG RFHCQCPPGR VGPTCADEKS PCQPNPCHGA APCRVLPEGG AQCECPLGRE 1620
GTFCQTASGQ DGSGPFLADF NGFSHLELRG LHTFARDLGE KMALEVVFLA RGPSGLLLYN 1680
GQKTDGKGDF VSLALRDRRL EFRYDLGKGA AVIRSREPVT LGAWTRVSLE RNGRKGALRV 1740
GDGPRVLGES PKSRKVPHTV LNLKEPLYVG GAPDFSKLAR AAAVSSGFDG AIQLVSLGGR 1800
QLLTPEHVLR QVDVTSFAGH PCTRASGHPC LNGASCVPRE AAYVCLCPGG FSGPHCEKGL 1860
VEKSAGDVDT LAFDGRTFVE YLNAVTESEL ANEIPVPETL DSGALHEKAL QSNHFELSLR 1920
TEATQGLVLW SGKATERADY VALAIVDGHL QLSYNLGSQP VVLRSTVPVN TNRWLRVVAH 1980
REQREGSLQV GNEAPVTGSS PLGATQLDTD GALWLGGLPE LPVGPALPKA YGTGFVGCLR 2040
DVVVGRHPLH LLEDAVTKPE LRPCPTP 2068
Keyword

KW-0025--Alternative splicing
KW-0106--Calcium
KW-0965--Cell junction
KW-1003--Cell membrane
KW-0181--Complete proteome
KW-1004--Congenital myasthenic syndrome
KW-0217--Developmental protein
KW-0221--Differentiation
KW-0225--Disease mutation
KW-1015--Disulfide bond
KW-0245--EGF-like domain
KW-0272--Extracellular matrix
KW-0325--Glycoprotein
KW-0357--Heparan sulfate
KW-0424--Laminin EGF-like domain
KW-0472--Membrane
KW-0597--Phosphoprotein
KW-0621--Polymorphism
KW-0654--Proteoglycan
KW-1185--Reference proteome
KW-0677--Repeat
KW-0964--Secreted
KW-0732--Signal
KW-0770--Synapse
KW-0812--Transmembrane
KW-1133--Transmembrane helix
Interpro

IPR013320--ConA-like_dom
IPR013032--EGF-like_CS
IPR000742--EGF-like_dom
IPR003645--Fol_N
IPR002350--Kazal_dom
IPR002049--Laminin_EGF
IPR001791--Laminin_G
IPR004850--NtA_dom
IPR000082--SEA_dom
IPR008993--TIMP-like_OB-fold
PROSITE

PS00022--EGF_1
PS01186--EGF_2
PS50026--EGF_3
PS01248--EGF_LAM_1
PS50027--EGF_LAM_2
PS51465--KAZAL_2
PS50025--LAM_G_DOMAIN
PS51121--NTA
PS50024--SEA
Pfam

PF00008--EGF
PF00050--Kazal_1
PF07648--Kazal_2
PF00053--Laminin_EGF
PF00054--Laminin_G_1
PF03146--NtA
PF01390--SEA
Gene Ontology

GO:0005605--C:basal lamina
GO:0030054--C:cell junction
GO:0009986--C:cell surface
GO:0005737--C:cytoplasm
GO:0070062--C:extracellular exosome
GO:0031012--C:extracellular matrix
GO:0005576--C:extracellular region
GO:0005615--C:extracellular space
GO:0005796--C:Golgi lumen
GO:0016021--C:integral component of membrane
GO:0043202--C:lysosomal lumen
GO:0005886--C:plasma membrane
GO:0045202--C:synapse
GO:0030548--F:acetylcholine receptor regulator activity
GO:0005509--F:calcium ion binding
GO:0035374--F:chondroitin sulfate binding
GO:0002162--F:dystroglycan binding
GO:0043395--F:heparan sulfate proteoglycan binding
GO:0043236--F:laminin binding
GO:0033691--F:sialic acid binding
GO:0005200--F:structural constituent of cytoskeleton
GO:0007411--P:axon guidance
GO:0005975--P:carbohydrate metabolic process
GO:0030204--P:chondroitin sulfate metabolic process
GO:0045162--P:clustering of voltage-gated sodium channels
GO:0030198--P:extracellular matrix organization
GO:0007213--P:G-protein coupled acetylcholine receptor signaling pathway
GO:0006024--P:glycosaminoglycan biosynthetic process
GO:0006027--P:glycosaminoglycan catabolic process
GO:0030203--P:glycosaminoglycan metabolic process
GO:0007528--P:neuromuscular junction development
GO:0045213--P:neurotransmitter receptor metabolic process
GO:0007603--P:phototransduction, visible light
GO:0007009--P:plasma membrane organization
GO:0051491--P:positive regulation of filopodium assembly
GO:0043547--P:positive regulation of GTPase activity
GO:0043525--P:positive regulation of neuron apoptotic process
GO:0045887--P:positive regulation of synaptic growth at neuromuscular junction
GO:0045944--P:positive regulation of transcription from RNA polymerase II promoter
GO:0043113--P:receptor clustering
GO:0001523--P:retinoid metabolic process
GO:0007165--P:signal transduction
GO:0044281--P:small molecule metabolic process
GO:0050808--P:synapse organization
GO:0007268--P:synaptic transmission