dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000097
Uniprot Accession O00459; P85B_HUMAN; Q5EAT5; Q9UPH9;
Genbank Protein ID NP_005018.1;
Genbank Nucleotide ID NM_005027.3;
Protein Name Phosphatidylinositol 3-kinase regulatory subunit beta
Protein Synonyms/Alias
Gene Name PIK3R2
Gene Synonyms/Alias
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)		 Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (By similarity).
Phosphorylation Sites
 dbPAF PTMs: 23 (View all)
PositionPeptidesSourceReferences ( PMIDs )
34PGDVLVVSRAALQALPhosphoSitePlus22135298
74RGDFPGTYVEFLGPVPhosphoSitePlus22135298
119PDLPEQFSPPDVAPPcurated20873877
262LRAPPPPSSPPPGGAdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;curated19369195; 16381945; 23193290; 18669648; 18691976; 18988627;
263RAPPPPSSPPPGGAPdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PHOSIDA;PhosphoSitePlus;curated19369195; 16381945; 23193290; 18669648; 18988627; 21081558;
365SSKIQGEYTLTLRKGdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoPep 2.0;PhosphoSitePlus;curated18083107; 16381945; 23193290; 18988627; 19060867; 22135298;
423KLDTRLLYPVSKYQQPhosphoSitePlus;curated22135298; 22817900
449VGAQLKVYHQQYQDKdbPTM 3.0;PhosphoSitePlus;curated20736484; 16381945; 23193290; 22135298; 22817900
453LKVYHQQYQDKSREYdbPTM 3.0;PhosphoSitePlus;curated20736484; 16381945; 23193290; 22135298; 22817900
457HQQYQDKSREYDQLYPhosphoSitePlus22135298
460YQDKSREYDQLYEEYdbPTM 3.0;HPRD 9;PhosphoSitePlus16497976; 16381945; 23193290; 18988627; 22135298
464SREYDQLYEEYTRTSdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;UniProt;curated15592455; 15951569; 17389395; 18083107; 19534553; 16381945;
467YDQLYEEYTRTSQELdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoPep 2.0;PhosphoSitePlus;curated18083107; 16381945; 23193290; 16497976; 18180459; 20736484;
468DQLYEEYTRTSQELQdbPTM 3.0;HPRD 9;PHOSIDA;PhosphoSitePlus;curated20068231; 16381945; 23193290; 18988627; 21081558; 22135298
487AIEAFNETIKIFEEQdbPTM 3.0;HPRD 920068231; 16381945; 23193290; 18988627
505QEKCSKEYLERFRREcurated23898821
577LRKIRDQYLVWLTQKdbPTM 3.0;PhosphoSitePlus;curated20090780; 21049007; 16381945; 23193290; 22135298; 23898821;
582DQYLVWLTQKGARQKPhosphoSitePlus22135298
601WLGIKNETEDQYALMPhosphoSitePlus22135298
605KNETEDQYALMEDEDdbPTM 3.0;HPRD 9;PhosphoPep 2.0;PhosphoSitePlus;UniProt;curated17389395; 19690332; 16381945; 23193290; 17016520; 17646646;
Sequence
(Fasta)		MAGPEGFQYR ALYPFRRERP EDLELLPGDV LVVSRAALQA LGVAEGGERC PQSVGWMPGL 60
NERTRQRGDF PGTYVEFLGP VALARPGPRP RGPRPLPARP RDGAPEPGLT LPDLPEQFSP 120
PDVAPPLLVK LVEAIERTGL DSESHYRPEL PAPRTDWSLS DVDQWDTAAL ADGIKSFLLA 180
LPAPLVTPEA SAEARRALRE AAGPVGPALE PPTLPLHRAL TLRFLLQHLG RVASRAPALG 240
PAVRALGATF GPLLLRAPPP PSSPPPGGAP DGSEPSPDFP ALLVEKLLQE HLEEQEVAPP 300
ALPPKPPKAK PASTVLANGG SPPSLQDAEW YWGDISREEV NEKLRDTPDG TFLVRDASSK 360
IQGEYTLTLR KGGNNKLIKV FHRDGHYGFS EPLTFCSVVD LINHYRHESL AQYNAKLDTR 420
LLYPVSKYQQ DQIVKEDSVE AVGAQLKVYH QQYQDKSREY DQLYEEYTRT SQELQMKRTA 480
IEAFNETIKI FEEQGQTQEK CSKEYLERFR REGNEKEMQR ILLNSERLKS RIAEIHESRT 540
KLEQQLRAQA SDNREIDKRM NSLKPDLMQL RKIRDQYLVW LTQKGARQKK INEWLGIKNE 600
TEDQYALMED EDDLPHHEER TWYVGKINRT QAEEMLSGKR DGTFLIRESS QRGCYACSVV 660
VDGDTKHCVI YRTATGFGFA EPYNLYGSLK ELVLHYQHAS LVQHNDALTV TLAHPVRAPG 720
PGPPPAAR 729
Keyword

KW-0002--3D-structure
KW-0181--Complete proteome
KW-0225--Disease mutation
KW-0597--Phosphoprotein
KW-0621--Polymorphism
KW-0653--Protein transport
KW-1185--Reference proteome
KW-0677--Repeat
KW-0727--SH2 domain
KW-0728--SH3 domain
KW-0346--Stress response
KW-0813--Transport
KW-0832--Ubl conjugation
Interpro

IPR032498--PI3K_P85_iSH2
IPR001720--PI3kinase_P85
IPR008936--Rho_GTPase_activation_prot
IPR000198--RhoGAP_dom
IPR000980--SH2
IPR001452--SH3_domain
PROSITE

PS50238--RHOGAP
PS50001--SH2
PS50002--SH3
Pfam

PF16454--PI3K_P85_iSH2
PF00620--RhoGAP
PF00017--SH2
Gene Ontology

GO:0005829--C:cytosol
GO:0005634--C:nucleus
GO:0005942--C:phosphatidylinositol 3-kinase complex
GO:0046935--F:1-phosphatidylinositol-3-kinase regulator activity
GO:0046982--F:protein heterodimerization activity
GO:0019903--F:protein phosphatase binding
GO:0030971--F:receptor tyrosine kinase binding
GO:0007596--P:blood coagulation
GO:0001678--P:cellular glucose homeostasis
GO:0032869--P:cellular response to insulin stimulus
GO:0007173--P:epidermal growth factor receptor signaling pathway
GO:0038095--P:Fc-epsilon receptor signaling pathway
GO:0038096--P:Fc-gamma receptor signaling pathway involved in phagocytosis
GO:0008543--P:fibroblast growth factor receptor signaling pathway
GO:0045087--P:innate immune response
GO:0008286--P:insulin receptor signaling pathway
GO:0050900--P:leukocyte migration
GO:0048011--P:neurotrophin TRK receptor signaling pathway
GO:0014065--P:phosphatidylinositol 3-kinase signaling
GO:0006661--P:phosphatidylinositol biosynthetic process
GO:0046854--P:phosphatidylinositol phosphorylation
GO:0036092--P:phosphatidylinositol-3-phosphate biosynthetic process
GO:0048015--P:phosphatidylinositol-mediated signaling
GO:0006644--P:phospholipid metabolic process
GO:2001275--P:positive regulation of glucose import in response to insulin stimulus
GO:0042993--P:positive regulation of transcription factor import into nucleus
GO:0045944--P:positive regulation of transcription from RNA polymerase II promoter
GO:0015031--P:protein transport
GO:0010506--P:regulation of autophagy
GO:0043551--P:regulation of phosphatidylinositol 3-kinase activity
GO:0051056--P:regulation of small GTPase mediated signal transduction
GO:0034976--P:response to endoplasmic reticulum stress
GO:0007264--P:small GTPase mediated signal transduction
GO:0044281--P:small molecule metabolic process
GO:0050852--P:T cell receptor signaling pathway
GO:0048010--P:vascular endothelial growth factor receptor signaling pathway