dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000092
Uniprot Accession O00444; PLK4_HUMAN; B2RAL0; B7Z837; B7Z8G7; Q8IYF0; Q96Q95; Q9UD84; Q9UDE2;
Genbank Protein ID NP_001177728.1; NP_001177730.1; NP_055079.3;
Genbank Nucleotide ID NM_001190799.1; NM_001190801.1; NM_014264.4;
Protein Name Serine/threonine-protein kinase PLK4
Protein Synonyms/Alias Polo-like kinase 4;PLK-4;Serine/threonine-protein kinase 18;Serine/threonine-protein kinase Sak;
Gene Name PLK4
Gene Synonyms/Alias SAK;STK18;
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)		 Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2.
Phosphorylation Sites
 dbPAF PTMs: 35 (View all)
PositionPeptidesSourceReferences ( PMIDs )
22GNLLGKGSFAGVYRAdbPTM 3.0;PhosphoSitePlus21985771; 16381945; 23193290; 22135298
170MPHEKHYTLCGTPNYdbPTM 3.0;PhosphoSitePlus;curated11120772; 21985771; 16381945; 23193290; 22135298; 22817900
174KHYTLCGTPNYISPEPhosphoSitePlus22135298
177TLCGTPNYISPEIATdbPTM 3.0;PhosphoSitePlus21985771; 16381945; 23193290; 22135298
255RNPADRLSLSSVLDHPhosphoSitePlus22135298
257PADRLSLSSVLDHPFPhosphoSitePlus22135298
258ADRLSLSSVLDHPFMPhosphoSitePlus;curated22135298; 23090842
266VLDHPFMSRNSSTKSPhosphoSitePlus;curated22135298; 23090842
305SSTSISGSLFDKRRLdbPTM 3.0;PhosphoSitePlus;curated20032307; 16381945; 23193290; 22135298; 22817900
323QPLPNKMTVFPKNKSPhosphoSitePlus22135298
330TVFPKNKSSTDFSSSPhosphoSitePlus;curated22135298; 23917254
345GDGNSFYTQWGNQETPhosphoSitePlus22135298
377SRYLRRAYSSDRSGTdbPTM 3.0;PHOSIDA;PhosphoSitePlus;curated19369195; 16381945; 23193290; 21081558; 22135298; 22817900
378RYLRRAYSSDRSGTSdbPTM 3.0;PHOSIDA;PhosphoSitePlus;curated19369195; 16381945; 23193290; 21081558; 22135298; 23898821;
394SQSQAKTYTMERCHSPhosphoSitePlus22135298
401YTMERCHSAEMLSVSdbPTM 3.0;HPRD 9;PHOSIDA;PhosphoSitePlus;SysPTM 2.0;UniProt;curated18691976; 19369195; 16381945; 23193290; 18988627; 21081558;
406CHSAEMLSVSKRSGGPhosphoSitePlus22135298
420GGENEERYSPTDNNAdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus19369195; 16381945; 23193290; 19060867; 22135298
421GENEERYSPTDNNANdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;curated18691976; 19369195; 16381945; 23193290; 18669648; 18988627;
423NEERYSPTDNNANIFcurated25850435
Sequence
(Fasta)		MATCIGEKIE DFKVGNLLGK GSFAGVYRAE SIHTGLEVAI KMIDKKAMYK AGMVQRVQNE 60
VKIHCQLKHP SILELYNYFE DSNYVYLVLE MCHNGEMNRY LKNRVKPFSE NEARHFMHQI 120
ITGMLYLHSH GILHRDLTLS NLLLTRNMNI KIADFGLATQ LKMPHEKHYT LCGTPNYISP 180
EIATRSAHGL ESDVWSLGCM FYTLLIGRPP FDTDTVKNTL NKVVLADYEM PSFLSIEAKD 240
LIHQLLRRNP ADRLSLSSVL DHPFMSRNSS TKSKDLGTVE DSIDSGHATI STAITASSST 300
SISGSLFDKR RLLIGQPLPN KMTVFPKNKS STDFSSSGDG NSFYTQWGNQ ETSNSGRGRV 360
IQDAEERPHS RYLRRAYSSD RSGTSNSQSQ AKTYTMERCH SAEMLSVSKR SGGGENEERY 420
SPTDNNANIF NFFKEKTSSS SGSFERPDNN QALSNHLCPG KTPFPFADPT PQTETVQQWF 480
GNLQINAHLR KTTEYDSISP NRDFQGHPDL QKDTSKNAWT DTKVKKNSDA SDNAHSVKQQ 540
NTMKYMTALH SKPEIIQQEC VFGSDPLSEQ SKTRGMEPPW GYQNRTLRSI TSPLVAHRLK 600
PIRQKTKKAV VSILDSEEVC VELVKEYASQ EYVKEVLQIS SDGNTITIYY PNGGRGFPLA 660
DRPPSPTDNI SRYSFDNLPE KYWRKYQYAS RFVQLVRSKS PKITYFTRYA KCILMENSPG 720
ADFEVWFYDG VKIHKTEDFI QVIEKTGKSY TLKSESEVNS LKEEIKMYMD HANEGHRICL 780
ALESIISEEE RKTRSAPFFP IIIGRKPGST SSPKALSPPP SVDSNYPTRE RASFNRMVMH 840
SAASPTQAPI LNPSMVTNEG LGLTTTASGT DISSNSLKDC LPKSAQLLKS VFVKNVGWAT 900
QLTSGAVWVQ FNDGSQLVVQ AGVSSISYTS PNGQTTRYGE NEKLPDYIKQ KLQCLSSILL 960
MFSNPTPNFH 971
Keyword

KW-0002--3D-structure
KW-0025--Alternative splicing
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0206--Cytoskeleton
KW-0242--Dwarfism
KW-0418--Kinase
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-0621--Polymorphism
KW-1185--Reference proteome
KW-0723--Serine/threonine-protein kinase
KW-0808--Transferase
KW-0832--Ubl conjugation
Interpro

IPR011009--Kinase-like_dom
IPR000959--POLO_box_duplicated_dom
IPR000719--Prot_kinase_dom
IPR017441--Protein_kinase_ATP_BS
IPR002290--Ser/Thr_dual-sp_kinase
IPR008266--Tyr_kinase_AS
PROSITE

PS50078--POLO_BOX
PS00107--PROTEIN_KINASE_ATP
PS50011--PROTEIN_KINASE_DOM
Pfam

PF00069--Pkinase
PF00659--POLO_box
Gene Ontology

GO:0005814--C:centriole
GO:0005813--C:centrosome
GO:0032154--C:cleavage furrow
GO:0005829--C:cytosol
GO:0098536--C:deuterosome
GO:0005730--C:nucleolus
GO:0001741--C:XY body
GO:0005524--F:ATP binding
GO:0004674--F:protein serine/threonine kinase activity
GO:0007099--P:centriole replication
GO:0098535--P:de novo centriole assembly
GO:0000086--P:G2/M transition of mitotic cell cycle
GO:0000278--P:mitotic cell cycle
GO:0006996--P:organelle organization
GO:0046601--P:positive regulation of centriole replication
GO:0006468--P:protein phosphorylation
GO:0060707--P:trophoblast giant cell differentiation