dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000084
Uniprot Accession O00418; EF2K_HUMAN; Q8N588;
Genbank Protein ID NP_037434.1; XP_005276554.1;
Genbank Nucleotide ID NM_013302.3; XM_005276497.2;
Protein Name Eukaryotic elongation factor 2 kinase
Protein Synonyms/Alias
Gene Name EEF2K
Gene Synonyms/Alias
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)		 Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
Phosphorylation Sites
 dbPAF PTMs: 51 (View all)
PositionPeptidesSourceReferences ( PMIDs )
18EGVDGGQSPRAGHDGdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;UniProt;curated15144186; 16964243; 17081983; 18669648; 19413330; 19690332;
27RAGHDGDSDGDSDDEdbPTM 3.0;HPRD 9;PHOSIDA;PhosphoSitePlus;SysPTM 2.0;UniProt;curated17081983; 19413330; 16381945; 23193290; 18988627; 21081558;
31DGDSDGDSDDEEGYFdbPTM 3.0;HPRD 9;PHOSIDA;PhosphoSitePlus;SysPTM 2.0;curated17081983; 19413330; 16381945; 23193290; 18988627; 21081558;
37DSDDEEGYFICPITDPhosphoSitePlus22135298
59VNSKVNKYYSNLTKSPhosphoSitePlus22135298
60NSKVNKYYSNLTKSEPhosphoSitePlus22135298
61SKVNKYYSNLTKSERdbPTM 3.0;PhosphoSitePlus22216903; 16381945; 23193290; 22135298
66YYSNLTKSERYSSSGdbPTM 3.0;PhosphoSitePlus;curated22216903; 16381945; 23193290; 22135298; 25627689
69NLTKSERYSSSGSPAdbPTM 3.0;HPRD 9;PHOSIDA;SysPTM 2.0;curated17081983; 16381945; 23193290; 18988627; 21081558; 19366988;
70LTKSERYSSSGSPANdbPTM 3.0;HPRD 9;PHOSIDA;PhosphoSitePlus;SysPTM 2.0;curated17081983; 16381945; 23193290; 19276368; 18988627; 21081558;
71TKSERYSSSGSPANSPhosphoPep 2.0;PhosphoSitePlus;curated19060867; 22135298; 25850435; 25627689; 23898821; 20873877
72KSERYSSSGSPANSFdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoPep 2.0;PhosphoSitePlus;curated18669648; 16381945; 23193290; 21659604; 18988627; 19060867;
74ERYSSSGSPANSFHFdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoPep 2.0;PhosphoSitePlus;curated18669648; 16381945; 23193290; 19276368; 21659604; 22115753;
78SSGSPANSFHFKEAWdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoSitePlus;curated21112387; 16381945; 23193290; 14709557; 15024086; 22216903;
135EVLIKMASQPFGRGAdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoSitePlus;SysPTM 2.0;curated17287340; 16381945; 23193290; 18988627; 22135298; 19366988;
236EHYIEGKYIKYNSNSdbPTM 3.0;PhosphoSitePlus;curated18083107; 16381945; 23193290; 22135298; 22817900
241GKYIKYNSNSGFVRDPhosphoSitePlus22135298
243YIKYNSNSGFVRDDNPhosphoSitePlus;curated22135298; 23186163
340RDAVNQNTKLLQSAKPhosphoSitePlus22135298
345QNTKLLQSAKTILRGPhosphoSitePlus22135298
Sequence
(Fasta)		MADEDLIFRL EGVDGGQSPR AGHDGDSDGD SDDEEGYFIC PITDDPSSNQ NVNSKVNKYY 60
SNLTKSERYS SSGSPANSFH FKEAWKHAIQ KAKHMPDPWA EFHLEDIATE RATRHRYNAV 120
TGEWLDDEVL IKMASQPFGR GAMRECFRTK KLSNFLHAQQ WKGASNYVAK RYIEPVDRDV 180
YFEDVRLQME AKLWGEEYNR HKPPKQVDIM QMCIIELKDR PGKPLFHLEH YIEGKYIKYN 240
SNSGFVRDDN IRLTPQAFSH FTFERSGHQL IVVDIQGVGD LYTDPQIHTE TGTDFGDGNL 300
GVRGMALFFY SHACNRICES MGLAPFDLSP RERDAVNQNT KLLQSAKTIL RGTEEKCGSP 360
QVRTLSGSRP PLLRPLSENS GDENMSDVTF DSLPSSPSSA TPHSQKLDHL HWPVFSDLDN 420
MASRDHDHLD NHRESENSGD SGYPSEKRGE LDDPEPREHG HSYSNRKYES DEDSLGSSGR 480
VCVEKWNLLN SSRLHLPRAS AVALEVQRLN ALDLEKKIGK SILGKVHLAM VRYHEGGRFC 540
EKGEEWDQES AVFHLEHAAN LGELEAIVGL GLMYSQLPHH ILADVSLKET EENKTKGFDY 600
LLKAAEAGDR QSMILVARAF DSGQNLSPDR CQDWLEALHW YNTALEMTDC DEGGEYDGMQ 660
DEPRYMMLAR EAEMLFTGGY GLEKDPQRSG DLYTQAAEAA MEAMKGRLAN QYYQKAEEAW 720
AQMEE 726
Keyword

KW-0007--Acetylation
KW-0067--ATP-binding
KW-0106--Calcium
KW-0112--Calmodulin-binding
KW-0181--Complete proteome
KW-0418--Kinase
KW-0547--Nucleotide-binding
KW-0597--Phosphoprotein
KW-0621--Polymorphism
KW-1185--Reference proteome
KW-0723--Serine/threonine-protein kinase
KW-0808--Transferase
Interpro

IPR017400--eEF-2K
IPR011009--Kinase-like_dom
IPR004166--MHCK_EF2_kinase
IPR006597--Sel1-like
IPR011990--TPR-like_helical_dom
PROSITE

PS51158--ALPHA_KINASE
Pfam

PF02816--Alpha_kinase
PF08238--Sel1
Gene Ontology

GO:0005737--C:cytoplasm
GO:0005829--C:cytosol
GO:0005524--F:ATP binding
GO:0005509--F:calcium ion binding
GO:0005516--F:calmodulin binding
GO:0004686--F:elongation factor-2 kinase activity
GO:0004672--F:protein kinase activity
GO:0008135--F:translation factor activity, RNA binding
GO:0008286--P:insulin receptor signaling pathway
GO:0046777--P:protein autophosphorylation
GO:0031952--P:regulation of protein autophosphorylation
GO:0006414--P:translational elongation