dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000064
Uniprot Accession O00308; WWP2_HUMAN; A6NEP1; B2R706; B4DTL5; F5H213; H3BRF3; I3RSG8; Q6ZTQ5; Q96CZ2; Q9BWN6;
Genbank Protein ID NP_001257382.1; NP_001257383.1; NP_001257384.1; NP_008945.2; NP_955456.1; XP_011521125.1; XP_011521126.1; XP_011521127.1; XP_011521128.1;
Genbank Nucleotide ID NM_001270453.1; NM_001270454.1; NM_001270455.1; NM_007014.4; NM_199424.2; XM_011522823.1; XM_011522824.1; XM_011522825.1; XM_011522826.1;
Protein Name NEDD4-like E3 ubiquitin-protein ligase WWP2
Protein Synonyms/Alias Atrophin-1-interacting protein 2;AIP2;WW domain-containing protein 2;
Gene Name WWP2
Gene Synonyms/Alias
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)		 E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; in embryonic stem cells (ESCs) the ubiquitination is proposed to regulate POU5F1 protein level. Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation-induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation.
Phosphorylation Sites
 dbPAF PTMs: 19
PositionPeptidesSourceReferences ( PMIDs )
7-MASASSSRAGVALPdbPTM 3.0;HPRD 9;curated20068231; 16381945; 23193290; 18988627
18VALPFEKSQLTLKVVPhosphoSitePlus22135298
21PFEKSQLTLKVVSAKPhosphoSitePlus22135298
87HLDLKVWSCHTLRNEPhosphoSitePlus22135298
90LKVWSCHTLRNELLGPhosphoSitePlus22135298
193CFGGRSRTHRHSGASPhosphoSitePlus22135298
197RSRTHRHSGASARTTPhosphoSitePlus22135298
207SARTTPATGEQSPGAcurated25850435; 22817900
211TPATGEQSPGARSRHdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;UniProt;curated16964243; 19007248; 19413330; 16381945; 23193290; 19664995;
369TAEYVRNYEQWQSQRPhosphoSitePlus;curated22135298; 22817900
392HFSQRFLYQSSSASTPhosphoSitePlus22135298
455PGWEMKYTSEGVRYFdbPTM 3.0;HPRD 9;curated20068231; 16381945; 23193290; 18988627
467RYFVDHNTRTTTFKDdbPTM 3.0;HPRD 9;curated20068231; 16381945; 23193290; 18988627
469FVDHNTRTTTFKDPRdbPTM 3.0;HPRD 9;curated18691976; 16381945; 23193290; 18988627; 22817900
491KQGSPGAYDRSFRWKdbPTM 3.0;PhosphoSitePlus;curated18083107; 16381945; 23193290; 22135298; 22817900
528RQTLFEDSFQQIMNMcurated22817900
556RGEEGLDYGGIAREWPhosphoSitePlus22135298
587EYAGKNNYCLQINPAPhosphoSitePlus22135298
631TGFTLPFYKRMLNKRPhosphoSitePlus22135298
Sequence
(Fasta)		MASASSSRAG VALPFEKSQL TLKVVSAKPK VHNRQPRINS YVEVAVDGLP SETKKTGKRI 60
GSSELLWNEI IILNVTAQSH LDLKVWSCHT LRNELLGTAS VNLSNVLKNN GGKMENMQLT 120
LNLQTENKGS VVSGGELTIF LDGPTVDLGN VPNGSALTDG SQLPSRDSSG TAVAPENRHQ 180
PPSTNCFGGR SRTHRHSGAS ARTTPATGEQ SPGARSRHRQ PVKNSGHSGL ANGTVNDEPT 240
TATDPEEPSV VGVTSPPAAP LSVTPNPNTT SLPAPATPAE GEEPSTSGTQ QLPAAAQAPD 300
ALPAGWEQRE LPNGRVYYVD HNTKTTTWER PLPPGWEKRT DPRGRFYYVD HNTRTTTWQR 360
PTAEYVRNYE QWQSQRNQLQ GAMQHFSQRF LYQSSSASTD HDPLGPLPPG WEKRQDNGRV 420
YYVNHNTRTT QWEDPRTQGM IQEPALPPGW EMKYTSEGVR YFVDHNTRTT TFKDPRPGFE 480
SGTKQGSPGA YDRSFRWKYH QFRFLCHSNA LPSHVKISVS RQTLFEDSFQ QIMNMKPYDL 540
RRRLYIIMRG EEGLDYGGIA REWFFLLSHE VLNPMYCLFE YAGKNNYCLQ INPASSINPD 600
HLTYFRFIGR FIAMALYHGK FIDTGFTLPF YKRMLNKRPT LKDLESIDPE FYNSIVWIKE 660
NNLEECGLEL YFIQDMEILG KVTTHELKEG GESIRVTEEN KEEYIMLLTD WRFTRGVEEQ 720
TKAFLDGFNE VAPLEWLRYF DEKELELMLC GMQEIDMSDW QKSTIYRHYT KNSKQIQWFW 780
QVVKEMDNEK RIRLLQFVTG TCRLPVGGFA ELIGSNGPQK FCIDKVGKET WLPRSHTCFN 840
RLDLPPYKSY EQLREKLLYA IEETEGFGQE 871
Keyword

KW-0025--Alternative splicing
KW-0181--Complete proteome
KW-0945--Host-virus interaction
KW-0436--Ligase
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway
Interpro

IPR000008--C2_dom
IPR024928--E3_ub_ligase_SMURF1
IPR000569--HECT_dom
IPR001202--WW_dom
PROSITE

PS50237--HECT
PS01159--WW_DOMAIN_1
PS50020--WW_DOMAIN_2
Pfam

PF00632--HECT
PF00397--WW
Gene Ontology

GO:0005737--C:cytoplasm
GO:0070062--C:extracellular exosome
GO:0016020--C:membrane
GO:0005634--C:nucleus
GO:0000151--C:ubiquitin ligase complex
GO:0016874--F:ligase activity
GO:0001085--F:RNA polymerase II transcription factor binding
GO:0008134--F:transcription factor binding
GO:0001190--F:transcriptional activator activity, RNA polymerase II transcription factor binding
GO:0061630--F:ubiquitin protein ligase activity
GO:0004842--F:ubiquitin-protein transferase activity
GO:0006464--P:cellular protein modification process
GO:0010629--P:negative regulation of gene expression
GO:0051224--P:negative regulation of protein transport
GO:0043433--P:negative regulation of sequence-specific DNA binding transcription factor activity
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter
GO:0045892--P:negative regulation of transcription, DNA-templated
GO:0032410--P:negative regulation of transporter activity
GO:0043161--P:proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0051865--P:protein autoubiquitination
GO:0070534--P:protein K63-linked ubiquitination
GO:0016567--P:protein ubiquitination
GO:0042787--P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0034765--P:regulation of ion transmembrane transport
GO:0042391--P:regulation of membrane potential
GO:1901016--P:regulation of potassium ion transmembrane transporter activity
GO:0046718--P:viral entry into host cell