dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0000049
Uniprot Accession O00257; CBX4_HUMAN; B1PJR7; Q6TPI8; Q96C04;
Genbank Protein ID NP_003646.2;
Genbank Nucleotide ID NM_003655.2;
Protein Name E3 SUMO-protein ligase CBX4
Protein Synonyms/Alias Chromobox protein homolog 4;Polycomb 2 homolog;Pc2;hPc2;
Gene Name CBX4
Gene Synonyms/Alias
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)		 E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131.
Phosphorylation Sites
 dbPAF PTMs: 25 (View all)
PositionPeptidesSourceReferences ( PMIDs )
28IRKGRVEYLVKWRGWPhosphoSitePlus22135298
90VPTFARRSNVLTGLQdbPTM 3.0;HPRD 9;curated19007248; 16381945; 23193290; 18988627; 22817900
120GKGQGHQYELNSKKHPhosphoSitePlus22135298
150PPGKSGKYYYQLNSKPhosphoSitePlus22135298
151PGKSGKYYYQLNSKKPhosphoSitePlus22135298
152GKSGKYYYQLNSKKHPhosphoSitePlus22135298
169YQPDPKMYDLQYQGGPhosphoSitePlus22135298
182GGHKEAPSPTCPDLGdbPTM 3.0;HPRD 9;PhosphoSitePlus;curated19691289; 16381945; 23193290; 19413330; 18988627; 22135298;
207QGAGAKGYLGAVKPLdbPTM 3.0;PhosphoSitePlus18083107; 16381945; 23193290; 22135298
267GRIVIVMSKYMENGMdbPTM 3.0;HPRD 9;curated20068231; 16381945; 23193290; 18988627
291VAEGEARSPSHKKRAPHOSIDA;PhosphoSitePlus;curated21081558; 22135298; 25627689; 23898821; 20873877; 21857030;
293EGEARSPSHKKRAADdbPTM 3.0;HPRD 9;SysPTM 2.0;curated17081983; 16381945; 23193290; 18988627; 19366988; 23911959;
336EEEVSGVSDPQPQDAdbPTM 3.0;PhosphoSitePlus20044836; 16381945; 23193290; 22135298
345PQPQDAGSRKLSPTKPhosphoSitePlus;curated22135298; 26074081
349DAGSRKLSPTKEAFGdbPTM 3.0;HPRD 9;PHOSIDA;PhosphoSitePlus;curated20068231; 16381945; 23193290; 18669648; 18988627; 21081558;
351GSRKLSPTKEAFGEQdbPTM 3.0;HPRD 9;curated18669648; 16381945; 23193290; 18988627; 22817900; 23836654;
415HVRKRCLSETHGEREdbPTM 3.0;HPRD 9;PHOSIDA;PhosphoSitePlus;SysPTM 2.0;curated17081983; 16381945; 23193290; 18988627; 21081558; 22135298;
417RKRCLSETHGEREPCdbPTM 3.0;PhosphoSitePlus19276368; 16381945; 23193290; 22135298
429EPCKKRLTARSISTPcurated26074081
432KKRLTARSISTPTCLdbPTM 3.0;PhosphoSitePlus;curated18220336; 16381945; 23193290; 22135298; 20873877; 23663014
Sequence
(Fasta)		MELPAVGEHV FAVESIEKKR IRKGRVEYLV KWRGWSPKYN TWEPEENILD PRLLIAFQNR 60
ERQEQLMGYR KRGPKPKPLV VQVPTFARRS NVLTGLQDSS TDNRAKLDLG AQGKGQGHQY 120
ELNSKKHHQY QPHSKERAGK PPPPGKSGKY YYQLNSKKHH PYQPDPKMYD LQYQGGHKEA 180
PSPTCPDLGA KSHPPDKWAQ GAGAKGYLGA VKPLAGAAGA PGKGSEKGPP NGMMPAPKEA 240
VTGNGIGGKM KIVKNKNKNG RIVIVMSKYM ENGMQAVKIK SGEVAEGEAR SPSHKKRAAD 300
ERHPPADRTF KKAAGAEEKK VEAPPKRREE EVSGVSDPQP QDAGSRKLSP TKEAFGEQPL 360
QLTTKPDLLA WDPARNTHPP SHHPHPHPHH HHHHHHHHHH AVGLNLSHVR KRCLSETHGE 420
REPCKKRLTA RSISTPTCLG GSPAAERPAD LPPAAALPQP EVILLDSDLD EPIDLRCVKT 480
RSEAGEPPSS LQVKPETPAS AAVAVAAAAA PTTTAEKPPA EAQDEPAESL SEFKPFFGNI 540
IITDVTANCL TVTFKEYVTV 561
Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0025--Alternative splicing
KW-0156--Chromatin regulator
KW-0181--Complete proteome
KW-1017--Isopeptide bond
KW-0436--Ligase
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0678--Repressor
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway
Interpro

IPR000953--Chromo/shadow_dom
IPR017984--Chromo_dom_subgr
IPR023780--Chromo_domain
IPR016197--Chromodomain-like
IPR023779--Chromodomain_CS
PROSITE

PS00598--CHROMO_1
PS50013--CHROMO_2
Pfam

PF00385--Chromo
Gene Ontology

GO:0016607--C:nuclear speck
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0031519--C:PcG protein complex
GO:0035102--C:PRC1 complex
GO:0003682--F:chromatin binding
GO:0019899--F:enzyme binding
GO:0016874--F:ligase activity
GO:0003727--F:single-stranded RNA binding
GO:0032183--F:SUMO binding
GO:0019789--F:SUMO transferase activity
GO:0003714--F:transcription corepressor activity
GO:0044212--F:transcription regulatory region DNA binding
GO:0044267--P:cellular protein metabolic process
GO:0016568--P:chromatin modification
GO:0043066--P:negative regulation of apoptotic process
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter
GO:0045892--P:negative regulation of transcription, DNA-templated
GO:0043687--P:post-translational protein modification
GO:0016925--P:protein sumoylation
GO:0006351--P:transcription, DNA-templated