dbPAF Protein Information


Tag Content
dbPAF ID dbPAF-0000020
Uniprot Accession O00180; KCNK1_HUMAN; Q13307; Q5T5E8;
Genbank Protein ID NP_002236.1;
Genbank Nucleotide ID NM_002245.3;
Protein Name Potassium channel subfamily K member 1
Protein Synonyms/Alias Inward rectifying potassium channel protein TWIK-1;Potassium channel K2P1;Potassium channel KCNO1;
Gene Name KCNK1
Gene Synonyms/Alias HOHO1;KCNO1;TWIK1;
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)
Ion channel that contributes to passive transmembrane potassium transport and to the regulation of the resting membrane potential in brain astrocytes, but also in kidney and in other tissues (PubMed:15820677, PubMed:21653227). Forms dimeric channels through which potassium ions pass in accordance with their electrochemical gradient. The channel is selective for K(+) ions at physiological potassium concentrations and at neutral pH, but becomes permeable to Na(+) at subphysiological K(+) levels and upon acidification of the extracellular medium (PubMed:21653227, PubMed:22431633). The homodimer has very low potassium channel activity, when expressed in heterologous systems, and can function as weakly inward rectifying potassium channel (PubMed:8605869, PubMed:8978667, PubMed:15820677, PubMed:21653227, PubMed:22431633, PubMed:23169818, PubMed:25001086). Channel activity is modulated by activation of serotonin receptors (By similarity). Heterodimeric channels containing KCNK1 and KCNK2 have much higher activity, and may represent the predominant form in astrocytes (By similarity). Heterodimeric channels containing KCNK1 and KCNK3 or KCNK9 have much higher activity (PubMed:23169818). Heterodimeric channels formed by KCNK1 and KCNK9 may contribute to halothane-sensitive currents (PubMed:23169818). Mediates outward rectifying potassium currents in dentate gyrus granule cells and contributes to the regulation of their resting membrane potential (By similarity). Contributes to the regulation of action potential firing in dentate gyrus granule cells and down-regulates their intrinsic excitability (By similarity). In astrocytes, the heterodimer formed by KCNK1 and KCNK2 is required for rapid glutamate release in response to activation of G-protein coupled receptors, such as F2R and CNR1 (By similarity). Required for normal ion and water transport in the kidney (By similarity). Contributes to the regulation of the resting membrane potential of pancreatic beta cells (By similarity). The low channel activity of homodimeric KCNK1 may be due to sumoylation (PubMed:15820677, PubMed:20498050, PubMed:23169818). The low channel activity may be due to rapid internalization from the cell membrane and retention in recycling endosomes (PubMed:19959478).
Phosphorylation Sites
dbPAF PTMs: 6
PositionPeptidesSourceReferences ( PMIDs )
4----MLQSLAGSSCVPhosphoSitePlus22135298
8MLQSLAGSSCVRLVEPhosphoSitePlus22135298
157TAVVQRITVHVTRRPPhosphoSitePlus22135298
300IIEHDQLSFSSITDQPhosphoSitePlus22135298
302EHDQLSFSSITDQAAPhosphoSitePlus22135298
326EPFVATQSSACVDGPPhosphoPep 2.019060867
Sequence
(Fasta)
MLQSLAGSSC VRLVERHRSA WCFGFLVLGY LLYLVFGAVV FSSVELPYED LLRQELRKLK 60
RRFLEEHECL SEQQLEQFLG RVLEASNYGV SVLSNASGNW NWDFTSALFF ASTVLSTTGY 120
GHTVPLSDGG KAFCIIYSVI GIPFTLLFLT AVVQRITVHV TRRPVLYFHI RWGFSKQVVA 180
IVHAVLLGFV TVSCFFFIPA AVFSVLEDDW NFLESFYFCF ISLSTIGLGD YVPGEGYNQK 240
FRELYKIGIT CYLLLGLIAM LVVLETFCEL HELKKFRKMF YVKKDKDEDQ VHIIEHDQLS 300
FSSITDQAAG MKEDQKQNEP FVATQSSACV DGPANH 337
Keyword

KW-0002--3D-structure
KW-0965--Cell junction
KW-1003--Cell membrane
KW-0966--Cell projection
KW-0181--Complete proteome
KW-0968--Cytoplasmic vesicle
KW-1015--Disulfide bond
KW-0967--Endosome
KW-0325--Glycoprotein
KW-0407--Ion channel
KW-0406--Ion transport
KW-1017--Isopeptide bond
KW-0472--Membrane
KW-0597--Phosphoprotein
KW-0630--Potassium
KW-0631--Potassium channel
KW-0633--Potassium transport
KW-1185--Reference proteome
KW-0770--Synapse
KW-0812--Transmembrane
KW-1133--Transmembrane helix
KW-0813--Transport
KW-0832--Ubl conjugation

Interpro

IPR003280--2pore_dom_K_chnl
IPR003092--2pore_dom_K_chnl_TASK
IPR005408--2pore_dom_K_chnl_TWIK
IPR001779--2pore_dom_K_chnl_TWIK1
IPR013099--K_chnl_dom

PROSITE
Pfam

PF07885--Ion_trans_2

Gene Ontology

GO:0016324--C:apical plasma membrane
GO:0030054--C:cell junction
GO:0016023--C:cytoplasmic membrane-bounded vesicle
GO:0030425--C:dendrite
GO:0016021--C:integral component of membrane
GO:0005887--C:integral component of plasma membrane
GO:1902937--C:inward rectifier potassium channel complex
GO:0043204--C:perikaryon
GO:0005886--C:plasma membrane
GO:0034705--C:potassium channel complex
GO:0055037--C:recycling endosome
GO:0045202--C:synapse
GO:0008076--C:voltage-gated potassium channel complex
GO:0005242--F:inward rectifier potassium channel activity
GO:0005267--F:potassium channel activity
GO:0022841--F:potassium ion leak channel activity
GO:0005272--F:sodium channel activity
GO:0071805--P:potassium ion transmembrane transport
GO:0006813--P:potassium ion transport
GO:0060075--P:regulation of resting membrane potential
GO:0035725--P:sodium ion transmembrane transport
GO:0030322--P:stabilization of membrane potential
GO:0007268--P:synaptic transmission